{"id":1026,"date":"2020-12-03T10:59:55","date_gmt":"2020-12-03T01:59:55","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1026"},"modified":"2021-01-19T12:18:12","modified_gmt":"2021-01-19T03:18:12","slug":"q519","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/protein-reporter\/ppi\/nanobret\/q519","title":{"rendered":"NanoBiT\u3068\u306e\u4f7f\u3044\u5206\u3051\u306f\uff1f"},"content":{"rendered":"\n<p>\u3053\u3061\u3089\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<figure class=\"wp-block-table\">\n    <table>\n        <tbody>\n            <tr>\n                <td colspan=\"3\" style=\"background: #ccc;\">\u3053\u306e\u3088\u3046\u306a\u5834\u5408\u3001NanoBRET or NanoBiT\uff1f<\/td>\n            <\/tr>\n            <tr>\n                <td>\u5b9f\u9a13\u6761\u4ef6<\/td>\n                <td>\u63a8\u5968\u624b\u6cd5<\/td>\n                <td>\u7406\u7531<\/td>\n            <\/tr>\n            <tr>\n                <td>Peptide &#8211; Protein binding<\/td>\n                <td>NanoBiT\u2122<\/td>\n                <td>NanoBRET\u2122\u306e\u5834\u5408\u3001Peptide\u306b NanoLuc(19KDa) \u3042\u308b\u3044\u306fHaloTag(32KDa)\u3092\u6a19\u8b58\u3059\u308b\u3068peptide\u306e\u6d3b\u6027\u306b\u5f71\u97ff\u304c\u51fa\u308b\u53ef\u80fd\u6027<\/td>\n            <\/tr>\n            <tr>\n                <td>\u4f4e\u89aa\u548c\u6027 (\u60f3\u5b9aKd &gt;10\u03bcM)<\/td>\n                <td>NanoBRET\u2122<\/td>\n                <td>SmBit: LgBit\u306e\u89aa\u548c\u6027\u304c190\u03bcM \u3067\u3042\u308a\u3001\u6a19\u7684\u30bf\u30f3\u30d1\u30af\u9593\u306e\u89aa\u548c\u6027\u304c\u4f4e\u3044\u3068\u3001SmBiT:LgBiT\u306e\u89aa\u548c\u6027\u304c\u95a2\u4e0e\u3057\u3066\u3057\u307e\u3046<\/td>\n            <\/tr>\n            <tr>\n                <td>\u5206\u6ce8\u518d\u73fe\u6027\uff08\u3070\u3089\u3064\u304d\uff09\u306b\u81ea\u4fe1\u304c\u306a\u3044<\/td>\n                <td>NanoBRET\u2122<\/td>\n                <td>ratio metric \u306a\u306e\u3067\u3001\u3070\u3089\u3064\u304d\u304c\u88dc\u6b63\u3055\u308c\u308b<\/td>\n            <\/tr>\n            <tr>\n                <td>High Throughput<\/td>\n                <td>NanoBiT\u2122<\/td>\n                <td>\u767a\u5149\u8a66\u85ac\u306e\u6dfb\u52a0\u30b9\u30c6\u30c3\u30d7\u304cBit\u306f1\u56de\u3001BRET\u306f2\u56de\u3001\u6e2c\u5b9a\u3082BiT\u306f1\u56de\u3001BRET\u306f2\u56de<\/td>\n            <\/tr>\n            <tr>\n                <td>\u5358\u7d14\u306aLuminometer \u3057\u304b\u306a\u3044<\/td>\n                <td>NanoBiT\u2122<\/td>\n                <td>\u30c1\u30e5\u30fc\u30d6\u30eb\u30df\u30ce\u30e1\u30fc\u30bf\u30fc\u3067\u3082\u53ef\u80fd\uff08\u6d6e\u904a\u7d30\u80de\u306b\u9650\u308b\uff09<\/td>\n            <\/tr>\n            <tr>\n                <td>Imaging\u3067\u89b3\u5bdf\u3057\u305f\u3044<\/td>\n                <td>NanoBiT\u2122<\/td>\n                <td>NanoLuc \u306e 1\/3\u306e\u8f1d\u5ea6\u3092\u78ba\u4fdd\u3002NanoBRET\u306f\u3001BRET\u306b\u3088\u308b\u8ee2\u79fb\u52b9\u7387\u7b49\u306b\u3088\u308a\u8f1d\u5ea6\u304c\u671f\u5f85\u3067\u304d\u306a\u3044\u4e0a\u3001\u6700\u9069\u306a\u30d5\u30a3\u30eb\u30bf\u30fc\u304c\u5fc5\u8981\u3002<\/td>\n            <\/tr><\/tbody><\/table><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>\u3053\u3061\u3089\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002 \u3053\u306e\u3088\u3046\u306a\u5834\u5408\u3001NanoBRET or NanoBiT\uff1f \u5b9f\u9a13\u6761\u4ef6 \u63a8\u5968\u624b\u6cd5 \u7406\u7531 Peptide &#8211; Protein binding NanoBiT\u2122 NanoBRET\u2122\u306e\u5834\u5408\u3001P &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[198],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1026"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1026"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1026\/revisions"}],"predecessor-version":[{"id":2225,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1026\/revisions\/2225"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1026"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1026"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1026"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}