{"id":1070,"date":"2020-12-03T12:03:11","date_gmt":"2020-12-03T03:03:11","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1070"},"modified":"2021-01-19T12:32:46","modified_gmt":"2021-01-19T03:32:46","slug":"q534","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/cell-signaling\/cyclic-amp\/glosensor-camp-assay\/q534","title":{"rendered":"GloSensor\u2122 cAMP Assay\u306f\u3069\u306e\u3088\u3046\u306a\u30a2\u30c3\u30bb\u30a4\u3067\u3059\u304b\uff1f"},"content":{"rendered":"\n<p>GloSensor\u2122 cAMPP Assay\u306f\u3001GloSensor\u2122 cAMP\u30d0\u30a4\u30aa\u30bb\u30f3\u30b5\u30fc\u3092\u7d30\u80de\u306b\u767a\u73fe\u3055\u305b\u3001\u751f\u7d30\u80de\u5185cAMP\u30ec\u30d9\u30eb\u306e\u5909\u52d5\u3092\u6e2c\u5b9a\u3059\u308b\u65b0\u3057\u3044\u30a2\u30c3\u30bb\u30a4\u3067\u3059\u3002GloSensor\u2122 cAMP\u30d0\u30a4\u30aa\u30bb\u30f3\u30b5\u30fc\u306f\u3001\u30db\u30bf\u30eb\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u306e\u5185\u90e8\u306bcAMP\u7d50\u5408\u30c9\u30e1\u30a4\u30f3\u3092\u633f\u5165\u3057\u305f\u6539\u5909\u578b\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u3067\u3042\u308a\u3001cAMP\u7d50\u5408\u30c9\u30e1\u30a4\u30f3\u306bcAMP\u304c\u7d50\u5408\u3059\u308b\u3068\u69cb\u9020\u304c\u5909\u5316\u3057\u3066\u30eb\u30b7\u30d5\u30a7\u30ea\u30f3\u3068\u53cd\u5fdc\u3057\u3001\u767a\u5149\u91cf\u304c\u5897\u52a0\u3057\u307e\u3059 (\u56f31\u53c2\u7167)\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"480\" height=\"221\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/pic_GloSensor_01.gif\" alt=\"\" class=\"wp-image-2228\"\/><figcaption>\u56f31\u3000GloSensor\u2122 cAMPP \u30d0\u30a4\u30aa\u30bb\u30f3\u30b5\u30fc<\/figcaption><\/figure>\n\n\n\n<p>\u3053\u306e\u767a\u5149\u91cf\u306e\u5909\u5316\u306f\u53ef\u9006\u7684\u3067\u3042\u308a\u3001\u56f32\u306e\u3088\u3046\u306b\u6642\u9593\u7d4c\u904e\u307e\u305f\u306f\u30a2\u30f3\u30bf\u30b4\u30cb\u30b9\u30c8\u51e6\u7406\u306a\u3069\u306b\u3088\u308bcAMP\u6fc3\u5ea6\u306e\u4f4e\u4e0b\u306b\u4f34\u3063\u3066\u901f\u3084\u304b\u306b\u767a\u5149\u5024\u304c\u6e1b\u5c11\u3057\u307e\u3059\u3002\u8a73\u3057\u304f\u306f\u53c2\u8003\u6587\u732e(1)\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"377\" height=\"314\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/pic_GloSensor_02.gif\" alt=\"\" class=\"wp-image-2229\"\/><figcaption>\u56f32\u3000\u53ef\u9006\u7684\u306acAMP\u5909\u5316\u306e\u691c\u51fa<\/figcaption><\/figure>\n\n\n\n<p>GloSensor\u2122 cAMP\u30d0\u30a4\u30aa\u30bb\u30f3\u30b5\u30fc\u3092HEK293\u7d30\u80de\u306b\u4e00\u904e\u6027\u306b\u767a\u73fe\u3055\u305b\u300137\u2103\u306710 \u00b5M Isoproterenol (ISO)\u3001\u307e\u305f\u306f10 \u00b5M Forskolin (FSK)\u3067\u5185\u5728\u6027\u03b22\u30a2\u30c9\u30ec\u30ca\u30ea\u30f3\u53d7\u5bb9\u4f53\u3092\u523a\u6fc0\u3057\u305f\u3002\u300cMOD\u300d\u30b5\u30f3\u30d7\u30eb\u306f10 \u00b5M Isoproterenol\u523a\u6fc0\u5f8c\u3055\u3089\u306b10 \u00b5M Propranolol (PRO)\u300110 \u00b5M FSK\u3067\u9023\u7d9a\u3057\u3066\u523a\u6fc0\u3057\u305f(n=3)\u3002<\/p>\n\n\n\n<p>\u53c2\u8003\u6587\u732e\uff1a1. Fan, F. et al. (2008) ACS Chem. Biol. 3, 346-51.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>GloSensor\u2122 cAMPP Assay\u306f\u3001GloSensor\u2122 cAMP\u30d0\u30a4\u30aa\u30bb\u30f3\u30b5\u30fc\u3092\u7d30\u80de\u306b\u767a\u73fe\u3055\u305b\u3001\u751f\u7d30\u80de\u5185cAMP\u30ec\u30d9\u30eb\u306e\u5909\u52d5\u3092\u6e2c\u5b9a\u3059\u308b\u65b0\u3057\u3044\u30a2\u30c3\u30bb\u30a4\u3067\u3059\u3002GloSensor\u2122 cAMP\u30d0\u30a4\u30aa\u30bb\u30f3\u30b5\u30fc\u306f\u3001\u30db\u30bf &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[204],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1070"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1070"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1070\/revisions"}],"predecessor-version":[{"id":2230,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1070\/revisions\/2230"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1070"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1070"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1070"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}