{"id":1168,"date":"2020-12-03T12:29:48","date_gmt":"2020-12-03T03:29:48","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1168"},"modified":"2021-02-16T12:01:06","modified_gmt":"2021-02-16T03:01:06","slug":"q648","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/cell-health\/viability\/viability-endopoint-assay\/celltiter-glo\/q648","title":{"rendered":"CellTiter-Glo\u00ae \u30a2\u30c3\u30bb\u30a4\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u8981\u56e0\u3068\u3057\u3066\u3069\u306e\u3088\u3046\u306a\u3082\u306e\u304c\u6319\u3052\u3089\u308c\u307e\u3059\u304b?"},"content":{"rendered":"\n

1) \u6e29\u5ea6<\/strong>
CellTiter-Glo\u00ae<\/sup> \u306f\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u53cd\u5fdc\u3092\u5229\u7528\u3057\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u6e29\u5ea6\u306f\u91cd\u8981\u306a\u8981\u56e0\u306b\u306a\u308a\u307e\u3059\u3002\u30a2\u30c3\u30bb\u30a4\u306e\u524d\u306bCellTiter-Glo\u00ae<\/sup> Reagent\u3068\u57f9\u990a\u30d7\u30ec\u30fc\u30c8\u3092\u5fc5\u305a\u5ba4\u6e29\u306b\u623b\u3057\u3066\u304f\u3060\u3055\u3044\u3002
\u30d0\u30c3\u30d5\u30a1\u30fc\u6210\u5206\u3092\u542b\u307e\u306a\u3044\u57f9\u5730\u3092\u4f7f\u3063\u3066\u3044\u308b\u5834\u5408\u3001CO2\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u304b\u3089\u53d6\u308a\u51fa\u3057\u305f\u30d7\u30ec\u30fc\u30c8\u306e\u57f9\u5730\u306f\u3001pH\u304c\u4e0a\u304c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u5834\u5408\u306b\u306f\u3001\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u304b\u3089\u53d6\u308a\u51fa\u3057\u305f\u76f4\u5f8c\u306bCellTiter-Glo\u00ae<\/sup> Reagent\u3092\u52a0\u3048\u3001\u305d\u306e\u5f8c\u3001\u5341\u5206\u306b\u5ba4\u6e29\u306b\u623b\u3063\u3066\u304b\u3089\u767a\u5149\u5024\u3092\u6e2c\u5b9a\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

2) \u57f9\u5730\u306e\u7a2e\u985e<\/strong>
\u30d7\u30ed\u30e1\u30ac\u3067\u306f\u3001DMEM\u3001F12\u3001F12\/DME\u3001MEM\u3001RPMI 1640\u3092\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u3002DMEM\u3001F12\u3001F12\/DME\u3001MEM\u3067\u306f\u767a\u5149\u91cf\u306b\u5dee\u304c\u3042\u308a\u307e\u305b\u3093\u3067\u3057\u305f\u304c\u3001RPMI 1640\u306f\u88fd\u9020\u5143\u3084\u30ed\u30c3\u30c8\u306e\u9055\u3044\u306b\u3088\u308a\u5dee\u3092\u751f\u3058\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n

3) \u6709\u6a5f\u6eb6\u5a92<\/strong>
\u6709\u6a5f\u6eb6\u5a92\u306e\u6dfb\u52a0\u306b\u3088\u308a\u3001\u82e5\u5e72\u306e\u5f71\u97ff\u3092\u793a\u3059\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059(\u88685)\u3002\u3054\u5229\u7528\u306e\u7d30\u80de\u306b\u304a\u3044\u3066\u3001\u3042\u3089\u304b\u3058\u3081\u6709\u6a5f\u6eb6\u5a92\u306e\u5f71\u97ff\u3092\u3054\u78ba\u8a8d\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\u88685. \u6709\u6a5f\u6eb6\u5a92\u3068\u767a\u5149\u91cf\u306e\u95a2\u4fc2<\/strong><\/p>\n\n\n\n

\u6709\u6a5f\u6eb6\u5a92\u306e\u7d42\u6fc3\u5ea6<\/th>\u767a\u5149\u91cf\u306e\u5897\u6e1b<\/th><\/tr>
0.5-4.0% DMSO<\/td>5-20%\u306e\u767a\u5149\u91cf\u304c\u5897\u52a0<\/td><\/tr>
0.5-4.0% \u30b8\u30e1\u30c1\u30eb\u30db\u30eb\u30e0\u30a2\u30df\u30c9<\/td>\u307b\u3068\u3093\u3069\u5f71\u97ff\u306a\u3057<\/td><\/tr>
0.5-2.0% \u30a2\u30bb\u30c8\u30cb\u30c8\u30ea<\/td>\u307b\u3068\u3093\u3069\u5f71\u97ff\u306a\u3057<\/td><\/tr>
1.0-5.0% \u30a8\u30bf\u30ce\u30fc\u30eb<\/td>\u307b\u3068\u3093\u3069\u5f71\u97ff\u306a\u3057<\/td><\/tr>
7.5% \u30a8\u30bf\u30ce\u30fc\u30eb<\/td>15%\u306e\u767a\u5149\u91cf\u304c\u4f4e\u4e0b<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

4) \u8840\u6e05<\/strong>
\u30d7\u30ed\u30e1\u30ac\u3067\u306f\u3001\u4ed4\u30a6\u30b7(calf)\u8840\u6e05\u3001\u30a6\u30b7\u80ce\u5150\uff08fetal bovine\uff09\u8840\u6e05\u3001\u30a6\u30de\uff08hourse\uff09\u8840\u6e05\u3092\u542b\u3080\u3055\u307e\u3056\u307e\u306a\u57f9\u5730(DMEM\u3001F12\u3001F12\/DME\u3001MEM\u3001RPMI 1640)\u3067CellTiter-Glo\u00ae<\/sup> Reagent\u306e\u767a\u5149\u91cf\u3092\u8abf\u3079\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3092\u56f31\u306b\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56f31. \u767a\u5149\u91cf\u306b\u304a\u3051\u308b\u8840\u6e05\u306e\u5f71\u97ff<\/strong>
2\u03bcM ATP\u3092\u542b\u308050\u03bcl RPMI 1640\u306b\u300120%\u306e\u56f3\u4e2d\u306b\u793a\u3057\u305f\u8840\u6e05\u3092\u542b\u3080\u57f9\u5730\u3092\u52a0\u3048\u305f(\u8840\u6e05\u306e\u7d42\u6fc3\u5ea6\u306f10%)\u3002100\u03bcl\u306eCellTiter-Glo? Reagent\u3092\u52a0\u3048\u3001\u767a\u5149\u91cf\u3092\u6e2c\u5b9a\u3057\u305f\u3002\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306b\u306f\u30011\u03bcM ATP\u3092\u542b\u3080100\u03bcl DPBS\u3092\u4f7f\u3063\u305f\u3002<\/p>\n\n\n\n

5) \u30d5\u30a7\u30ce\u30fc\u30eb\u30ec\u30c3\u30c9<\/strong>
\u30d5\u30a7\u30ce\u30fc\u30eb\u30ec\u30c3\u30c9\u3092\u52a0\u3048\u305f\u57f9\u5730\u3092\u5229\u7528\u3059\u308b\u3053\u3068\u306f\u3001CellTiter-Glo\u00ae<\/sup> \u30a2\u30c3\u30bb\u30a4\u306b\u307b\u3068\u3093\u3069\u5f71\u97ff\u3057\u306a\u3044\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/p>\n\n\n\n

(\u53c2\u8003) 10.6mg\/mL\u306e\u30d5\u30a7\u30ce\u30fc\u30eb\u30ec\u30c3\u30c9\u3092\u52a0\u3048\u305f\u3068\u304d\u306b\u300110%\u4ee5\u4e0b\u306e\u767a\u5149\u91cf\u306e\u4f4e\u4e0b\u304c\u78ba\u8a8d\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

1) \u6e29\u5ea6CellTiter-Glo\u00ae \u306f\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u53cd\u5fdc\u3092\u5229\u7528\u3057\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u6e29\u5ea6\u306f\u91cd\u8981\u306a\u8981\u56e0\u306b\u306a\u308a\u307e\u3059\u3002\u30a2\u30c3\u30bb\u30a4\u306e\u524d\u306bCellTiter-Glo\u00ae Reagent\u3068\u57f9\u990a\u30d7\u30ec\u30fc\u30c8\u3092\u5fc5\u305a\u5ba4\u6e29\u306b\u623b\u3057\u3066 … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[217],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1168"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1168"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1168\/revisions"}],"predecessor-version":[{"id":2289,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1168\/revisions\/2289"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1168"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1168"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1168"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}