{"id":1201,"date":"2020-12-03T12:37:54","date_gmt":"2020-12-03T03:37:54","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1201"},"modified":"2021-02-16T12:03:53","modified_gmt":"2021-02-16T03:03:53","slug":"q658","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/cell-health\/viability\/viability-endopoint-assay\/celltiter96\/q658","title":{"rendered":"\u306e\u3088\u3046\u306bCellTiter96\u00ae \u306f\u6a5f\u80fd\u3057\u307e\u3059\u304b\u3002\u305d\u308c\u305e\u308c\u306e\u30d0\u30fc\u30b8\u30e7\u30f3\u306f\u3069\u306e\u3088\u3046\u306b\u9055\u3044\u307e\u3059\u304b\uff1f"},"content":{"rendered":"\n<p>CellTiter96<sup>\u00ae<\/sup>&nbsp;Assay\u306f\u3001\u751f\u7406\u6d3b\u6027\u306e\u3042\u308b\u7d30\u80de\u306b\u3088\u308b\u30c6\u30c8\u30e9\u30be\u30ea\u30a6\u30e0\u5869(MTT\u307e\u305f\u306fMTS)\u306e\u5b89\u5b9a\u306a\u30d5\u30a9\u30eb\u30de\u30b6\u30f3\u7523\u7269\u3078\u306e\u9084\u5143\u3092\u57fa\u672c\u3068\u3057\u3066\u3044\u307e\u3059\u3002\u30d5\u30a9\u30eb\u30de\u30b6\u30f3\u7523\u7269\u306f\u7279\u5b9a\u306e\u6ce2\u9577\u306e\u5149\u3092\u5438\u53ce\u3059\u308b\u305f\u3081\u3001\u6a19\u6e96\u7684\u306aELISA\u30d7\u30ec\u30fc\u30c8\u30ea\u30fc\u30c0\u30fc\u3067\u5b9a\u91cf\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u307b\u3068\u3093\u3069\u306e\u7cfb\u306b\u304a\u3044\u3066\u5438\u53ce\u306f\u57f9\u990a\u6db2\u4e2d\u306e\u751f\u7d30\u80de\u6570\u3068\u6bd4\u4f8b\u3057\u307e\u3059\u3002CellTiter96<sup>\u00ae<\/sup>&nbsp;Non-Radioactive Cell Proliferation Assay (\u30ab\u30bf\u30ed\u30b0\u756a\u53f7 G4000\u3001G4001)\u306f\u3001MTT\u3092\u542b\u3080Dye Solution\u3001\u754c\u9762\u6d3b\u6027\u5264\u3068\u6709\u6a5f\u6eb6\u5a92\u3092\u542b\u3080Solubilization\/Stop Solution\u304b\u3089\u306a\u308a\u307e\u3059\u3002Solubilization\/Stop Solution\u306f\u3001\u7d30\u80de\u306b\u3088\u308a\u9084\u5143\u3055\u308c\u305f\u30d5\u30a9\u30eb\u30de\u30b6\u30f3\u7523\u7269\u306e\u7d50\u6676\u3092\u6eb6\u89e3\u3059\u308b\u305f\u3081\u306b\u4f7f\u3044\u307e\u3059\u3002CellTiter96<sup>\u00ae<\/sup>&nbsp;AQueous Cell Proliferation Assay (\u30ab\u30bf\u30ed\u30b0\u756a\u53f7 G5421\u3001G5430\u3001G5440)\u3067\u306f\u3001\u30c6\u30c8\u30e9\u30be\u30ea\u30a6\u30e0\u8a66\u85ac\u3068\u3057\u3066MTT\u306e\u4ee3\u308a\u306bMTS\u3092\u4f7f\u3044\u307e\u3059\u3002MTS\u306f\u3001\u6709\u6a5f\u6eb6\u5a92\u306e\u6dfb\u52a0\u304c\u4e0d\u5fc5\u8981\u306a\u53ef\u6eb6\u6027\u306e\u30d5\u30a9\u30eb\u30de\u30b6\u30f3\u7523\u7269\u306b\u5909\u63db\u3055\u308c\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001CellTiter96<sup>\u00ae<\/sup>&nbsp;AQueous Assay System\u3092\u4f7f\u3063\u3066\u8abf\u6574\u3055\u308c\u305f\u30b5\u30f3\u30d7\u30eb\u306f\u3001\u6e2c\u5b9a\u5f8c\u3001\u3055\u3089\u306b\u767a\u8272\u3055\u305b\u305f\u3044\u6642\u306b\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u306b\u623b\u3057\u3066\u53cd\u5fdc\u3092\u9032\u3081\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002CellTiter96<sup>\u00ae<\/sup>&nbsp;AQueous Assay System\u306fMTS\u3068PMS(phenazine methosulfate;\u96fb\u5b50\u6355\u7372\u5264)\u3092\u5225\u3005\u306e\u30dc\u30c8\u30eb\u306b\u5165\u308c\u3066\u3044\u307e\u3059\u3002CellTiter96<sup>\u00ae<\/sup>&nbsp;AQueous One Solution Cell Proliferation Assay (\u30ab\u30bf\u30ed\u30b0\u756a\u53f7 G3580\u3001G3581\u3001G3582)\u3067\u306f\u3001\u7c21\u4fbf\u6027\u3092\u8003\u3048\u3001\u4e00\u672c\u306e\u30dc\u30c8\u30eb\u306b\u5b89\u5b9a\u306a\u6eb6\u6db2\u3068\u3057\u3066MTS\u3068PES(phenazine ethosulfate;\u96fb\u5b50\u6355\u7372\u5264)\u3092\u6df7\u5408\u3057\u3066\u5165\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<p>CellTiter96<sup>\u00ae<\/sup>\u3001CellTiter96<sup>\u00ae<\/sup>&nbsp;AQueous\u3001CellTiter96<sup>\u00ae<\/sup>&nbsp;AQueous One Solution Cell Proliferation Assay\u306e3\u7a2e\u985e\u306f\uff3b<sup>3<\/sup>H\uff3d\u30c1\u30df\u30b8\u30f3\u53d6\u308a\u8fbc\u307f\u8a66\u9a13\u306b\u5bfe\u3059\u308bNon-RI\u306e\u4ee3\u7528\u624b\u6cd5\u3068\u3057\u3066\u4f7f\u308f\u308c\u307e\u3059\u3002CellTiter96<sup>\u00ae<\/sup>&nbsp;Assay\u3067\u306f\u3001\u7d30\u80de\u3092\u307e\u304d\u306a\u304a\u3057\u305f\u308a\u57f9\u5730\u3092\u4ea4\u63db\u3059\u308b\u5fc5\u8981\u304c\u306a\u3044\u305f\u3081\u3001\u3088\u308a\u591a\u304f\u306e\u5b9f\u9a13\u624b\u9806\u3092\u5fc5\u8981\u3068\u3059\u308b\uff08\u7d50\u679c\u7684\u306b\u8aa4\u5dee\u304c\u5e83\u304c\u308b\u3053\u3068\u306b\u306a\u308b\uff09\uff3b<sup>3<\/sup>H\uff3d\u30c1\u30df\u30b8\u30f3\u53d6\u308a\u8fbc\u307f\u8a66\u9a13\u3068\u6bd4\u3079\u3001\u6a19\u6e96\u504f\u5dee\u304c\u5c0f\u3055\u304f\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p>[<sup>3<\/sup>H]\u30c1\u30df\u30b8\u30f3\u306e\u53d6\u308a\u8fbc\u307f\u3068\u751f\u7523\u3055\u308c\u308b\u30d5\u30a9\u30eb\u30de\u30b6\u30f3\u7523\u7269\u306e\u91cf\u3092\u3001\u7570\u306a\u308b\u30b5\u30f3\u30d7\u30eb\u9593\u306e\u6210\u9577\u56e0\u5b50\u306e\u91cf\u306e\u6e2c\u5b9a\u8a66\u9a13\u3067\u6bd4\u8f03\u3057\u305f\u3068\u304d\u3001\u9055\u3044\u306f5%\u4ee5\u4e0b\u3067\u3057\u305f\u3002\uff3b<sup>3<\/sup>H\uff3d\u30c1\u30df\u30b8\u30f3\u306e\u53d6\u308a\u8fbc\u307f\u306e\u7d50\u679c\u306f\u4e00\u822c\u7684\u306bcounts per minutes (cpm)\u3067\u8868\u3055\u308c\u307e\u3059\u3002\u4e00\u65b9\u3001CellTiter96<sup>\u00ae<\/sup>&nbsp;Cell Proliferation Assay\u306fMTT\u3067\u306f570-630nm\u3001MTS\u3067\u306f490nm\u3067\u6e2c\u5b9a\u3059\u308b\u5438\u5149\u5ea6\u3067\u8868\u3055\u308c\u307e\u3059\u3002\u54fa\u4e73\u985e(\u6d6e\u904a\u7d30\u80de\u3001\u4ed8\u7740\u7d30\u80de)\u3001\u690d\u7269\u3001\u9175\u6bcd\u306e\u57f9\u990a\u7d30\u80de\u3092\u542b\u3080\u307b\u3068\u3093\u3069\u306e\u771f\u6838\u751f\u7269\u3067\u5341\u5206\u306b\u5438\u5149\u5ea6\u3092\u5909\u5316\u3055\u305b\u308b\u3060\u3051\u306e\u7523\u7269\u3092\u4f5c\u308a\u307e\u3059\u3002\u3088\u308a\u4f4e\u3044\u4ee3\u8b1d\u6bd4\u7387\u3092\u6709\u3059\u308b\u3044\u304f\u3064\u304b\u306e\u7d30\u80de\u7a2e\u3067\u306f\u3001\u5341\u5206\u306a\u5438\u53ce\u5ea6\u3092\u5f97\u308b\u305f\u3081\u306b\u3001\u901a\u5e38\u3088\u308a\u591a\u304f\u306e\u7d30\u80de\u6570\u3092\u5fc5\u8981\u3068\u3057\u307e\u3059(5\uff5e25\u500d\u306e\u7d30\u80de\u6570)\u3002CellTiter96<sup>\u00ae<\/sup>\u306f\u7d30\u83cc\u3001\u83cc\u985e\u3001\u539f\u751f\u52d5\u7269\u306b\u3082\u4f7f\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n<p>MTS\u6cd5\u3092\u57fa\u306b\u3057\u305fCellTiter96<sup>\u00ae<\/sup>&nbsp;AQueous One Solution System\u306f\u8a66\u85ac\u3092\u201c\u52a0\u3048\u201d\u3001\u6e2c\u5b9a\u3059\u308b\u201d\u3060\u3051\u306e96\u30a6\u30a7\u30eb\u30bf\u30a4\u30d7\u306e\u8a66\u9a13\u3067\u3082\u3063\u3068\u3082\u4fbf\u5229\u306a\u65b9\u6cd5\u3067\u3059\u3002\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u306e\u5438\u5149\u5ea6\u3092\u304b\u306a\u308a\u4e0b\u3052\u305f\u3044\u3001\u307e\u305f\u306f\u30c7\u30fc\u30bf\u3092\u6e2c\u5b9a\u3059\u308b\u524d\u306b\u30d7\u30ec\u30fc\u30c8\u3092\u9577\u671f\u9593\u4fdd\u5b58\u3057\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u3044\u3088\u3046\u306a\u72b6\u6cc1\u3067\u306f\u3001MTT\u6cd5\u3092\u57fa\u306b\u3057\u305fCellTiter96<sup>\u00ae<\/sup>&nbsp;System\u3092\u304a\u3059\u3059\u3081\u3057\u307e\u3059\u3002(PN70-FAQ)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>CellTiter96\u00ae&nbsp;Assay\u306f\u3001\u751f\u7406\u6d3b\u6027\u306e\u3042\u308b\u7d30\u80de\u306b\u3088\u308b\u30c6\u30c8\u30e9\u30be\u30ea\u30a6\u30e0\u5869(MTT\u307e\u305f\u306fMTS)\u306e\u5b89\u5b9a\u306a\u30d5\u30a9\u30eb\u30de\u30b6\u30f3\u7523\u7269\u3078\u306e\u9084\u5143\u3092\u57fa\u672c\u3068\u3057\u3066\u3044\u307e\u3059\u3002\u30d5\u30a9\u30eb\u30de\u30b6\u30f3\u7523\u7269\u306f\u7279\u5b9a\u306e\u6ce2\u9577\u306e\u5149\u3092\u5438\u53ce\u3059\u308b\u305f\u3081\u3001\u6a19\u6e96\u7684\u306a &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[218],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1201"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1201"}],"version-history":[{"count":1,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1201\/revisions"}],"predecessor-version":[{"id":1202,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1201\/revisions\/1202"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1201"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1201"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1201"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}