{"id":1261,"date":"2020-12-03T12:52:14","date_gmt":"2020-12-03T03:52:14","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1261"},"modified":"2021-01-19T14:22:11","modified_gmt":"2021-01-19T05:22:11","slug":"q571","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/cell-signaling\/kinase-assay\/adp-glo\/q571","title":{"rendered":"\u63a8\u5968\u3059\u308b\u53cd\u5fdc\u6761\u4ef6\u306f\u3042\u308a\u307e\u3059\u304b\uff1f"},"content":{"rendered":"\n<p>ADP-Glo\u2122 Kinase Assay\u3067\u306f\u3001\u4ee5\u4e0b\u306e\u53cd\u5fdc\u6761\u4ef6\u3092\u63a8\u5968\u3057\u3066\u3044\u307e\u3059\u3002<br>\u53cd\u5fdc\u6db2\u7d44\u6210\uff1a 1X reaction buffer A (40mM Tris (pH 7.5), 20mM MgCl2, 0.1mg\/ml BSA) \uff0b \u30ad\u30ca\u30fc\u30bc \uff0b ATP \uff0b \u57fa\u8cea<br>\u203b\u30c1\u30ed\u30b7\u30f3\u30ad\u30ca\u30fc\u30bc\u307e\u305f\u306fI\u03baK\u03b2\u306e\u53cd\u5fdc\u306b\u306f\u30012mM DTT, 2mM MnCl2, 100\u00b5M sodium vanadate\u306e\u6dfb\u52a0\u3092\u63a8\u5968\u3057\u307e\u3059\u3002Sphingosine kinase\u306e\u53cd\u5fdc\u306b\u306f0.5mM DTT\u3092\u6dfb\u52a0\u3057\u3066\u4e0b\u3055\u3044\u3002<br><br>\u53cd\u5fdc\u6e29\u5ea6\uff1a\u5404\u30ad\u30ca\u30fc\u30bc\u306e\u81f3\u9069\u6e29\u5ea6<br>\u203bADP-Glo\u2122 Reagent\u6dfb\u52a0\u5f8c\u306f\u3001\u5ba4\u6e29\u3067\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3057\u3066\u4e0b\u3055\u3044 (<a href=\"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/cell-signaling\/kinase-assay\/adp-glo\/q574\">\u8a73\u3057\u304f\u306f\u3053\u3061\u3089<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044)\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>ADP-Glo\u2122 Kinase Assay\u3067\u306f\u3001\u4ee5\u4e0b\u306e\u53cd\u5fdc\u6761\u4ef6\u3092\u63a8\u5968\u3057\u3066\u3044\u307e\u3059\u3002\u53cd\u5fdc\u6db2\u7d44\u6210\uff1a 1X reaction buffer A (40mM Tris (pH 7.5), 20mM MgCl2, 0.1mg\/ml  &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[207],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1261"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1261"}],"version-history":[{"count":3,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1261\/revisions"}],"predecessor-version":[{"id":2249,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1261\/revisions\/2249"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1261"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1261"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1261"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}