{"id":1264,"date":"2020-12-03T12:52:42","date_gmt":"2020-12-03T03:52:42","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1264"},"modified":"2021-01-19T14:22:55","modified_gmt":"2021-01-19T05:22:55","slug":"q572","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/cell-signaling\/kinase-assay\/adp-glo\/q572","title":{"rendered":"ADP\u6fc3\u5ea6\u3068RLU\u5024\u306e\u76f8\u95a2\u6027\u3092\u8abf\u3079\u308b\u305f\u3081\u306b\u306f\u3069\u3046\u3059\u308c\u3070\u3088\u3044\u3067\u3059\u304b\uff1f"},"content":{"rendered":"\n

\u30ad\u30c3\u30c8\u306b\u6dfb\u4ed8\u3055\u308c\u3066\u3044\u308bATP\u3068ADP\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3092\u4f7f\u7528\u3057\u3066\u6a19\u6e96\u66f2\u7dda\u3092\u4f5c\u6210\u3057\u3001ADP\u91cf\u3068\u767a\u5149\u5024\u306e\u95a2\u4fc2\u3092\u8abf\u3079\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002ADP\u306e\u307f\u306e\u6a19\u6e96\u66f2\u7dda\u3092\u4f5c\u6210\u3059\u308b\u306e\u3067\u306f\u306a\u304f\u3001\u53cd\u5fdc\u4e2d\u306eATP\u306e\u4f55%\u304cADP\u306b\u5909\u63db\u3055\u308c\u305f\u304b\u3092\u8abf\u3079\u308b\u305f\u3081\u306bATP\u3068ADP\u306e\u6bd4\u7387\u3092\u5909\u3048\u3066\u6df7\u5408\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u3067\u6a19\u6e96\u66f2\u7dda\u3092\u4f5c\u6210\u3057\u307e\u3059\u3002
\u4f8b\u3068\u3057\u3066\u30011mM ATP-ADP\u5909\u63db\u66f2\u7dda\u306e\u4f5c\u6210\u65b9\u6cd5\u3092\u793a\u3057\u307e\u3059\u3002\u30ad\u30c3\u30c8\u306b\u6dfb\u4ed8\u3055\u308c\u3066\u3044\u308bUltra Pure ATP, 10mM\u3068ADP, 10mM\u3092Kinase Reaction Buffer (\u5b9f\u969b\u306b\u30ad\u30ca\u30fc\u30bc\u53cd\u5fdc\u306b\u4f7f\u7528\u3059\u308b\u3082\u306e)\u3067\u5e0c\u91c8\u3057\u3001\u88681\u306e\u3088\u3046\u306b\u5404\u6fc3\u5ea6\u306eATP\/ADP\u6df7\u5408\u6db2\u3092\u8abf\u88fd\u3057\u307e\u3059\u3002\u5404\u6fc3\u5ea6\u306eATP\/ADP\u6df7\u5408\u6db225\u00b5l\u306bADP-Glo\u2122 Reagent\u309225\u00b5l\u305a\u3064\u52a0\u3048\u306640\u5206\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3057\u307e\u3059\u3002\u305d\u306e\u5f8cKinase Detection Reagent\u309250\u00b5l\u305a\u3064\u52a0\u3048\u300140\u5206\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3057\u305f\u306e\u3061\u3001\u30eb\u30df\u30ce\u30e1\u30fc\u30bf\u30fc\u3067\u767a\u5149\u3092\u6e2c\u5b9a\u3057\u3066\u4e0b\u3055\u3044\u3002<\/p>\n\n\n\n

% ATP<\/th>100<\/td>80<\/td>60<\/td>40<\/td>20<\/td>10<\/td>5<\/td>4<\/td>3<\/td>2<\/td>1<\/td>0<\/td><\/tr>
1mM ADP (\u00b5l)<\/th>100<\/td>80<\/td>60<\/td>40<\/td>20<\/td>10<\/td>5<\/td>4<\/td>3<\/td>2<\/td>1<\/td>0<\/td><\/tr>
1mM ATP (\u00b5l)<\/th>0<\/td>20<\/td>40<\/td>60<\/td>80<\/td>90<\/td>95<\/td>96<\/td>97<\/td>98<\/td>99<\/td>100<\/td><\/tr>
% ADP<\/th>0<\/td>20<\/td>40<\/td>60<\/td>80<\/td>90<\/td>95<\/td>96<\/td>97<\/td>98<\/td>99<\/td>100<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

\u88681\u3000ATP-ADP\u5909\u63db\u66f2\u7dda\u306e\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd
<\/p>\n\n\n\n

\"\"
\u56f33\u3000 1mM ATP-ADP\u5909\u63db\u66f2\u7dda<\/figcaption><\/figure>\n","protected":false},"excerpt":{"rendered":"

\u30ad\u30c3\u30c8\u306b\u6dfb\u4ed8\u3055\u308c\u3066\u3044\u308bATP\u3068ADP\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3092\u4f7f\u7528\u3057\u3066\u6a19\u6e96\u66f2\u7dda\u3092\u4f5c\u6210\u3057\u3001ADP\u91cf\u3068\u767a\u5149\u5024\u306e\u95a2\u4fc2\u3092\u8abf\u3079\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002ADP\u306e\u307f\u306e\u6a19\u6e96\u66f2\u7dda\u3092\u4f5c\u6210\u3059\u308b\u306e\u3067\u306f\u306a\u304f\u3001\u53cd\u5fdc\u4e2d\u306eATP\u306e\u4f55%\u304cADP\u306b\u5909\u63db\u3055\u308c\u305f\u304b\u3092\u8abf\u3079\u308b\u305f\u3081\u306b … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[207],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1264"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1264"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1264\/revisions"}],"predecessor-version":[{"id":2251,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1264\/revisions\/2251"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1264"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1264"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1264"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}