{"id":1316,"date":"2020-12-03T14:19:10","date_gmt":"2020-12-03T05:19:10","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1316"},"modified":"2021-01-19T14:49:10","modified_gmt":"2021-01-19T05:49:10","slug":"q591","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/cell-signaling\/kinase-assay\/kinase-glo\/q591","title":{"rendered":"ATP\u6fc3\u5ea6\u3068RLU\u5024\u306e\u76f8\u95a2\u6027\u3092\u8abf\u3079\u308b\u305f\u3081\u306b\u306f\u3069\u3046\u3059\u308c\u3070\u3088\u3044\u3067\u3059\u304b\uff1f"},"content":{"rendered":"\n<p>rATP (Cat# P1132, 0.5mL, 10mM) \u30920.001\u03bcM\uff5e10\u03bcM\u306e\u7bc4\u56f2\u3068\u306a\u308b\u3088\u3046\u306b\u3001ATPase Free \u306e\u30ad\u30ca\u30fc\u30bcbuffer\u3067\u5e0c\u91c8\u3057\u307e\u3059\u3002\u3053\u306eATP\u6eb6\u6db250\u03bcL\u3068Kinase-Glo<sup>\u00ae<\/sup> \u8a66\u85ac\u309250\u03bcL\u52a0\u3048\u306625\u2103\u300110\u5206\u9593\u53cd\u5fdc\u5f8c\u3001Luciferase\u306e\u767a\u5149\u91cf\u3092\u30eb\u30df\u30ce\u30e1\u30fc\u30bf\u30fc\u3067\u6e2c\u5b9a\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u56f33\u306bATP\u6fc3\u5ea6\u3068RLU\u5024\u306e\u76f8\u95a2\u6027\u3092\u793a\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"350\" height=\"250\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/dia_kinase-glo_02.gif\" alt=\"\" class=\"wp-image-2261\"\/><figcaption>\u56f33\u3000ATP\u6fc3\u5ea6\u3068RLU\u5024\u306e\u76f8\u95a2\u6027<\/figcaption><\/figure>\n\n\n\n<blockquote class=\"wp-block-quote\"><p>50\u03bcl kinase reaction buffer (40mM Tris [pH 7.5], 20mM MgCl<sub>2<\/sub>, 0.1 mg\/ml BSA) \u306brATP\u3092\u5e0c\u91c8\u3057\u3001Kinase-Glo<sup>\u00ae<\/sup> \u8a66\u85ac\u3092\u6dfb\u52a010\u5206\u5f8c\u306b\u30eb\u30df\u30ce\u30e1\u30fc\u30bf\u30fc\u3067\u6e2c\u5b9a<\/p><\/blockquote>\n","protected":false},"excerpt":{"rendered":"<p>rATP (Cat# P1132, 0.5mL, 10mM) \u30920.001\u03bcM\uff5e10\u03bcM\u306e\u7bc4\u56f2\u3068\u306a\u308b\u3088\u3046\u306b\u3001ATPase Free \u306e\u30ad\u30ca\u30fc\u30bcbuffer\u3067\u5e0c\u91c8\u3057\u307e\u3059\u3002\u3053\u306eATP\u6eb6\u6db250\u03bcL\u3068Kinase-Glo\u00ae \u8a66\u85ac &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[208],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1316"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1316"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1316\/revisions"}],"predecessor-version":[{"id":2263,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1316\/revisions\/2263"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1316"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1316"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1316"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}