{"id":134,"date":"2020-12-01T14:31:51","date_gmt":"2020-12-01T05:31:51","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=134"},"modified":"2021-02-16T12:58:07","modified_gmt":"2021-02-16T03:58:07","slug":"q45","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/nucleic-acid-purification\/plasmid\/p-column\/wizard-plus-sv-mini\/q45","title":{"rendered":"Wizard\u00ae Plus SV Minipreps System\u3067\u7cbe\u88fd\u3057\u305f\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u81ea\u52d5\u86cd\u5149DNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u306b\u4f7f\u3048\u307e\u3059\u304b?"},"content":{"rendered":"\n<p>\u81ea\u52d5\u86cd\u5149DNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u3067\u306f\u3001\u9069\u5207\u306a\u6fc3\u5ea6\u7bc4\u56f2\u3067\u7cbe\u88fd\u3055\u308c\u305f\u30d7\u30e9\u30b9\u30df\u30c9\u304c\u5fc5\u8981\u3067\u3059\u3002\u7406\u60f3\u7684\u306a\u6fc3\u5ea6\u306f0.2\u03bcg\/\u03bcl\u3067\u3042\u308a\u30010.1\u03bcg\/\u03bcl\u4ee5\u4e0b\u3067\u306f\u30b7\u30fc\u30af\u30a8\u30f3\u30b7\u30f3\u30b0\u3067\u304d\u307e\u305b\u3093\u3002pGEM<sup>\u00ae<\/sup>&nbsp;Vector\u304c\u5f62\u8cea\u8ee2\u63db\u3055\u308c\u305fDH5\u03b1\u30921.5ml LB\u57f9\u5730\u3067\u57f9\u990a\u3057\u3001Wizard<sup>\u00ae<\/sup>&nbsp;<em>Plus<\/em>&nbsp;SV Minipreps System\u3067\u7cbe\u88fd\u3057\u305f\u5834\u5408\u3001\u901a\u5e383.5-5.0\u03bcg \u306e\u30d7\u30e9\u30b9\u30df\u30c9DNA\u304c\u5f97\u3089\u308c\u307e\u3059\u3002Wizard<sup>\u00ae<\/sup>&nbsp;<em>Plus<\/em>&nbsp;SV System \u3092\u4f7f\u3048\u3070\u3001EndA- \u3068EndA+\u306e\u3069\u3061\u3089\u304b\u3089\u7cbe\u88fd\u3057\u305fDNA\u3067\u3082ABI PRISMR BigDye\u2122\u30bf\u30fc\u30df\u30cd\u30fc\u30bf\u30fc\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u3092\u7528\u3044\u305f\u81ea\u52d5\u5316\u86cd\u5149DNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u306b\u9069\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<p>terminator ready reaction mix\u306e\u5e0c\u91c8\u3092\u884c\u3046\u5834\u5408\u3001D.W.\u3067\u306f\u306a\u304f\u9069\u3057\u305f\u30d0\u30c3\u30d5\u30a1\u30fc(250mM Tris-HCl [pH 9.0], 10mM MgCl<sub>2<\/sub>)\u3067\u5e0c\u91c8\u3059\u308b\u3053\u3068\u304c\u91cd\u8981\u3067\u3059\u3002\u9ad8\u30b3\u30d4\u30fc\u6570\u30d7\u30e9\u30b9\u30df\u30c9\u30926\u500d\u5e0c\u91c8\u3057\u305fterminator ready reaction mix\u3067\u89e3\u6790\u3057\u305f\u5834\u5408\u3001500\u5869\u57fa\u4ee5\u4e0a\u306e\u5224\u8aad\u304c\u53ef\u80fd\u3067\u3042\u308a\u300198%\u304b\u305d\u308c\u4ee5\u4e0a\u306e\u6b63\u78ba\u3055\u3067\u5224\u8aad\u3067\u304d\u307e\u3059\u3002\u5e0c\u91c8\u306e\u65b9\u6cd5\u306b\u3064\u3044\u3066\u306f\u3001Wizard<sup>\u00ae<\/sup>&nbsp;<em>Plus<\/em>&nbsp;SV Minipreps System\u306e\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb(TB225)\u306b\u8a18\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u3067\u306f\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u306b\u30a8\u30bf\u30ce\u30fc\u30eb\u6c88\u6bbf\u306f\u4e0d\u8981\u3067\u3059\u3002\uff08eNotes-FAQ0010)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u81ea\u52d5\u86cd\u5149DNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u3067\u306f\u3001\u9069\u5207\u306a\u6fc3\u5ea6\u7bc4\u56f2\u3067\u7cbe\u88fd\u3055\u308c\u305f\u30d7\u30e9\u30b9\u30df\u30c9\u304c\u5fc5\u8981\u3067\u3059\u3002\u7406\u60f3\u7684\u306a\u6fc3\u5ea6\u306f0.2\u03bcg\/\u03bcl\u3067\u3042\u308a\u30010.1\u03bcg\/\u03bcl\u4ee5\u4e0b\u3067\u306f\u30b7\u30fc\u30af\u30a8\u30f3\u30b7\u30f3\u30b0\u3067\u304d\u307e\u305b\u3093\u3002pGEM\u00ae&nbsp;Vector\u304c\u5f62\u8cea\u8ee2\u63db\u3055\u308c\u305f &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[71],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/134"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=134"}],"version-history":[{"count":1,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/134\/revisions"}],"predecessor-version":[{"id":135,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/134\/revisions\/135"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=134"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=134"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=134"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}