{"id":1348,"date":"2020-12-03T15:00:42","date_gmt":"2020-12-03T06:00:42","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1348"},"modified":"2021-08-16T18:11:32","modified_gmt":"2021-08-16T09:11:32","slug":"%e7%b4%b0%e8%83%9e%e3%83%a9%e3%82%a4%e3%82%bb%e3%83%bc%e3%83%88%e3%82%92%e4%bd%9c%e3%81%a3%e3%81%a6%e3%82%a2%e3%83%83%e3%82%bb%e3%82%a4%e3%81%a7%e3%81%8d%e3%81%be%e3%81%99%e3%81%8b%ef%bc%9f","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/genetic-reporter\/nano-luc\/nano-glo-luciferase\/%e7%b4%b0%e8%83%9e%e3%83%a9%e3%82%a4%e3%82%bb%e3%83%bc%e3%83%88%e3%82%92%e4%bd%9c%e3%81%a3%e3%81%a6%e3%82%a2%e3%83%83%e3%82%bb%e3%82%a4%e3%81%a7%e3%81%8d%e3%81%be%e3%81%99%e3%81%8b%ef%bc%9f","title":{"rendered":"\u7d30\u80de\u30e9\u30a4\u30bb\u30fc\u30c8\u3092\u4f5c\u3063\u3066\u30a2\u30c3\u30bb\u30a4\u3067\u304d\u307e\u3059\u304b\uff1f"},"content":{"rendered":"\n<p>\u53ef\u80fd\u3067\u3059\u3002<br>\u30d7\u30ed\u30e1\u30ac\u306e\u5404\u7d30\u80de\u6eb6\u89e3\u30d0\u30c3\u30d5\u30a1\u3067\u4f5c\u88fd\u3057\u305f\u30e9\u30a4\u30bb\u30fc\u30c8\u306b\u3001\u7b49\u91cf\u306eNano-Glo<sup>\u00ae<\/sup>&nbsp;Luciferase Assay Reagent\u3092\u6dfb\u52a0\u3057\u3066\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<br><br><strong>Glo Lysis Buffer \u3067\u306e\u30e9\u30a4\u30bb\u30fc\u30c8\u8abf\u88fd\u30fb\u6e2c\u5b9a\u4f8b\uff0896\u30a6\u30a7\u30eb\u30d7\u30ec\u30fc\u30c8\uff09<\/strong><\/p>\n\n\n\n<ol><li>\u30a6\u30a7\u30eb\u304b\u3089\u57f9\u5730\u3092\u9664\u304d\u3001PBS\u3067\u6d17\u6d44\u3059\u308b\u3002<\/li><li>PBS\u3092\u9664\u304d\u3001Glo Lysis Buffer (GLB)\uff08\u30ab\u30bf\u30ed\u30b0\u756a\u53f7\uff1aE2661\uff09\u3092100\u03bcl\u52a0\u3048\u308b\u3002<\/li><li>\u5ba4\u6e29\u30675\u5206\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3059\u308b\u3002<\/li><li>\u4f5c\u88fd\u3057\u305f\u30e9\u30a4\u30bb\u30fc\u30c8100\u03bcl\u3092\u6e2c\u5b9a\u7528\u30c1\u30e5\u30fc\u30d6\u307e\u305f\u306f\u30d7\u30ec\u30fc\u30c8\u306b\u79fb\u3059\u3002\u203b1<\/li><li>\u7b49\u91cf\u306eNano-Glo<sup>\u00ae<\/sup>&nbsp;Luciferase Assay Reagent\u3092\u6dfb\u52a0\u3057\u3001\u7de9\u3084\u304b\u306b\u6df7\u5408\u3059\u308b\u3002<\/li><li>3 \u5206\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3059\u308b\u3002<\/li><li>\u30eb\u30df\u30ce\u30e1\u30fc\u30bf\u30fc\u3067\u767a\u5149\u5024\u3092\u6e2c\u5b9a\u3059\u308b\u3002<\/li><\/ol>\n\n\n\n<p>\u203b1\uff1a\u79fb\u3059\u91cf\u306f\u5fc5\u8981\u306b\u5fdc\u3058\u3066\u5909\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u5404\u7d30\u80de\u6eb6\u89e3\u30d0\u30c3\u30d5\u30a1\u306e\u9055\u3044\u306b\u3064\u3044\u3066\u306f\u3001<a href=\"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/genetic-reporter\/cell-lysate\/cclr-rlb-plb-glb\/q457\">\u53c2\u8003Q&amp;A<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<br>\u8a73\u7d30\u306f<a href=\"https:\/\/www.promega.jp\/-\/media\/files\/resources\/protocols\/technical-manuals\/101\/nanoglo-luciferase-assay-system-protocol.pdf\">\u30de\u30cb\u30e5\u30a2\u30eb<\/a>8\u30da\u30fc\u30b8\u3001Figure 5\u3092\u3054\u78ba\u8a8d\u4e0b\u3055\u3044\u3002<\/p>\n\n\n\n<p><strong>\u25cf\u30e9\u30a4\u30bb\u30fc\u30c8\u306e\u51cd\u7d50\u4fdd\u5b58<\/strong><br>Glo Lysis Buffer \u3067\u4f5c\u88fd\u3057\u305f\u30e9\u30a4\u30bb\u30fc\u30c8\u306f\u3001-20\u2103\u3067\u4fdd\u5b58\u53ef\u80fd\u3057\u3066\uff12\u304b\u6708\u5f8c\u307e\u3067\u3001\u6d3b\u6027\u4f4e\u4e0b\u306a\u304f\u6e2c\u5b9a\u3067\u304d\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u4ee5\u4e0a\u9577\u671f\u306e\u4fdd\u5b58\u3084\u3001\u51cd\u7d50\u878d\u89e3\u306b\u3064\u3044\u3066\u306f\u3001\u3042\u3089\u304b\u3058\u3081\u304a\u8a66\u3057\u306e\u3046\u3048\u3054\u5229\u7528\u304f\u3060\u3055\u3044\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u53ef\u80fd\u3067\u3059\u3002\u30d7\u30ed\u30e1\u30ac\u306e\u5404\u7d30\u80de\u6eb6\u89e3\u30d0\u30c3\u30d5\u30a1\u3067\u4f5c\u88fd\u3057\u305f\u30e9\u30a4\u30bb\u30fc\u30c8\u306b\u3001\u7b49\u91cf\u306eNano-Glo\u00ae&nbsp;Luciferase Assay Reagent\u3092\u6dfb\u52a0\u3057\u3066\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002 Glo Lysis Buffer \u3067\u306e &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[179],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1348"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1348"}],"version-history":[{"count":8,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1348\/revisions"}],"predecessor-version":[{"id":3219,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1348\/revisions\/3219"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1348"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1348"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1348"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}