{"id":1407,"date":"2020-12-03T15:34:08","date_gmt":"2020-12-03T06:34:08","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1407"},"modified":"2020-12-23T11:47:21","modified_gmt":"2020-12-23T02:47:21","slug":"q360","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/proteomics\/e-coli-extraction\/competent-cell\/single-step-competent-cells-krx\/q360","title":{"rendered":"KRX\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a9\u30fc\u30e1\u30fc\u30b7\u30e7\u30f3\u64cd\u4f5c\u3092\u7c21\u5358\u306b\u6559\u3048\u3066\u304f\u3060\u3055\u3044"},"content":{"rendered":"\n<p>\u6b21\u306e\u3088\u3046\u306a\u624b\u9806\u3067\u884c\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u7528\u610f\u3059\u308b\u3082\u306e<\/p>\n\n\n\n<ul><li>LB \u3042\u308b\u3044\u306f SOC \u57f9\u5730<\/li><li>LB \u30d7\u30ec\u30fc\u30c8\uff08\u9069\u5207\u306a\u6297\u751f\u7269\u8cea\u3092\u542b\u3080\u3082\u306e\uff09<\/li><li>IPTG (\u30ab\u30bf\u30ed\u30b0\u756a\u53f7 V3955)<\/li><li>X-Gal (\u30ab\u30bf\u30ed\u30b0\u756a\u53f7V3941)<\/li><\/ul>\n\n\n\n<ol><li>KRX\u3092-70\u2103\u304b\u3089\u53d6\u308a\u51fa\u3057\u3001\u6c37\u4e0a\u3067\u878d\u89e3\u3059\u308b\u3002<\/li><li>DNA(1\uff5e50ng)\u3092\u6dfb\u52a0\u3057\u3001\u8efd\u304f\u6df7\u5408\u3059\u308b\u3002\uff08\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u306f\u884c\u308f\u306a\u3044\u3053\u3068\u3002\uff09<br>\u6ce8\u610f\uff1a\u300050\u00b5l\u306e\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u306b\u306f\u30015\u00b5l\u3001100\u00b5l\u306e\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u306b\u306f\u300110\u00b5l\u306e\u5bb9\u91cf\u3092\u8d85\u3048\u306a\u3044\u3088\u3046\u306bDNA\u6eb6\u6db2\u3092\u6dfb\u52a0\u3059\u308b\u3002<\/li><li>\u76f4\u3061\u306b\u6c37\u4e0a\u3067\u51b7\u5374\u3059\u308b\u3002\uff085\uff5e30\u5206\u9593\uff09<\/li><li>\u30d2\u30fc\u30c8\u30b7\u30e7\u30c3\u30af\u3092\u884c\u3046\u3002\uff0842\u2103\u306715-20\u79d2\u9593\uff09\u3000\uff08\u6df7\u5408\u306f\u3057\u306a\u3044\u3053\u3068\u3002\uff09<\/li><li>\u76f4\u3061\u306b\u6c37\u4e0a\u3067\u51b7\u5374\u3059\u308b\u3002\uff082\u5206\u9593\uff09<\/li><li>\u5ba4\u6e29\u306eSOC\u57f9\u5730\u3092\u6dfb\u52a0\u3057\u300137\u2103\u3067\u632f\u3068\u3046\uff08225rpm\uff09\u3057\u306a\u304c\u308960\u5206\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u3092\u884c\u3046\u3002<br>\u6ce8\u610f\uff1a\u300050\u00b5l\u306e\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u306b\u306f\u3001450\u00b5l\u3001100\u00b5l\u306e\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u306b\u306f\u3001900\u00b5l\u306eSOC\u57f9\u5730\u3092\u6dfb\u52a0\u3059\u308b\u3002<\/li><li>\u30d7\u30ec\u30fc\u30c8\u306b100\u00b5l\u3092\u5857\u5e03\u3059\u308b\u3002\uff08\u539f\u6db2\u304b\u3089100\u500d\u5e0c\u91c8\u307e\u3067\u306e\u3044\u304f\u3064\u304b\u306e\u6fc3\u5ea6\u6761\u4ef6\u3067\u884c\u3046\uff0937\u00b0C \u3067\u4e00\u6669\u57f9\u990a\u3059\u308b\u3002<\/li><\/ol>\n\n\n\n<p>\u8a73\u3057\u304f\u306f\u88fd\u54c1\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\uff08TB352\uff09\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u6b21\u306e\u3088\u3046\u306a\u624b\u9806\u3067\u884c\u3044\u307e\u3059\u3002 \u7528\u610f\u3059\u308b\u3082\u306e LB \u3042\u308b\u3044\u306f SOC \u57f9\u5730 LB \u30d7\u30ec\u30fc\u30c8\uff08\u9069\u5207\u306a\u6297\u751f\u7269\u8cea\u3092\u542b\u3080\u3082\u306e\uff09 IPTG (\u30ab\u30bf\u30ed\u30b0\u756a\u53f7 V3955) X-Gal (\u30ab\u30bf\u30ed\u30b0\u756a\u53f7V3941) KRX\u3092-70\u2103\u304b\u3089\u53d6\u308a &#8230; 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