{"id":1409,"date":"2020-12-03T15:36:52","date_gmt":"2020-12-03T06:36:52","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1409"},"modified":"2020-12-22T14:09:10","modified_gmt":"2020-12-22T05:09:10","slug":"q226","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/proteomics\/protein-tag\/gst\/magnegst-system\/q226","title":{"rendered":"MagneGST\u2122\u3068\u306f\u3069\u306e\u3088\u3046\u306a\u88fd\u54c1\u3067\u3059\u304b\uff1f \u307e\u305f\u3001\u3069\u306e\u3088\u3046\u306a\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u7cbe\u88fd\u306b\u5229\u7528\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u304b\uff1f"},"content":{"rendered":"\n<p>MagneGST\u2122 Protein Purification System(\u4ee5\u4e0bMagneGST\u2122)\u306f\u3001\u5927\u8178\u83cc\u3042\u308b\u3044\u306fin vitro\u7ffb\u8a33\u53cd\u5fdc\u5f8c\u3067\u767a\u73fe\u3055\u305b\u305f\u30b0\u30eb\u30bf\u30c1\u30aa\u30f3-S-\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30e9\u30fc\u30bc (GST) \u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea(\u4ee5\u4e0b\u3001GST-Tag\u30bf\u30f3\u30d1\u30af\u8cea)\u3092\u7c21\u5358\u3001\u8fc5\u901f\u3001\u5b89\u5b9a\u306b\u7cbe\u88fd\u3059\u308b\u305f\u3081\u306e\u30b7\u30b9\u30c6\u30e0\u3067\u3059\u3002MagneGST\u2122 \u306e\u7cbe\u88fd\u30d7\u30ed\u30c8\u30b3\u30eb\u306e\u6982\u8981\u3092\u56f31\u306b\u793a\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u78c1\u6027\u4f53\u7c92\u5b50\u3067\u3042\u308bMagneGST\u2122 Glutathione Particles\u306f\u3001GST-Tag\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u76f8\u4e92\u4f5c\u7528\u3057\u307e\u3059\uff081\uff09\u3002\u7d9a\u3044\u3066\u3001\u30de\u30b0\u30cd\u30c3\u30c8\u30b9\u30bf\u30f3\u30c9\u3092\u7528\u3044\u3066\u3001GST-Tag\u30bf\u30f3\u30d1\u30af\u8cea\u304cMagneGST\u2122 Glutathione Particles\u306b\u7d50\u5408\u3057\u305f\u72b6\u614b\u3067\u6d17\u6d44\u64cd\u4f5c\u3092\u884c\u3044\uff082\uff09\u3001Elution Buffer (10mM Glutathione\u3068\u306a\u308b\u3088\u3046\u306b\u8abf\u88fd\u3059\u308b)\u3067GST-Tag\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u6eb6\u51fa\u3055\u305b\u307e\u3059\uff083\uff09\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"450\" height=\"350\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/pic_MagneGST_01.gif\" alt=\"\" class=\"wp-image-1894\"\/><\/figure>\n\n\n\n<p>\u56f31 MagneGST\u2122 Protein Purification System\u306b\u3088\u308bGST-Tag\u30bf\u30f3\u30d1\u30af\u8cea\u7d50\u5408\u306e\u539f\u7406\u3068<br>\u7cbe\u88fd\u30d7\u30ed\u30c8\u30b3\u30eb\u306e\u6982\u8981<\/p>\n\n\n\n<p>MagneGST\u2122\u3092\u7528\u3044\u305fGST-Tag\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7cbe\u88fd\u306b\u306f\u3001\u5927\u8178\u83cc\u57f9\u990a\u6db2\u304b\u3089\u52b9\u7387\u7684\u306a\u30e9\u30a4\u30bb\u30fc\u30c8\u30b5\u30f3\u30d7\u30eb\u306e\u8abf\u88fd\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002\u30d7\u30ed\u30e1\u30ac\u3067\u306f\u3001OD600nm\u306e\u5438\u5149\u5024\u3068\u5927\u8178\u83cc\u57f9\u990a\u6db21mL\uff5e50mL\u307e\u3067\u306e\u30b9\u30b1\u30fc\u30eb\u306b\u5408\u308f\u305b\u3066\u9069\u5207\u306aMagneGST\u2122 Cell Lysis Reagent\u3068MagneGST\u2122 Glutathione Particles\u3092\u6dfb\u52a0\u3059\u308b\u3053\u3068\u3092\u52e7\u3081\u307e\u3059\uff08\u88681\uff09\u3002<\/p>\n\n\n\n<p>\u88681\u3000\u5927\u8178\u83cc\u57f9\u990a\u6db2\u91cf\u306b\u5bfe\u3057\u3066\u63a8\u5968\u3059\u308bMagneGST\u2122 Cell Lysis Regaent\u3068\u6dfb\u52a0\u91cf<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><th rowspan=\"2\">\u5927\u8178\u83cc\u57f9\u990a\u6db2\u91cf<\/th><th colspan=\"2\">MagneGST\u2122 Cell Lysis<br>Reagent\u6dfb\u52a0\u91cf<\/th><th rowspan=\"2\">MagneGST\u2122 Glutathione<br>Particles\u6dfb\u52a0\u91cf<\/th><\/tr><tr><th>OD<sub>600<\/sub>=2.0<\/th><th>OD<sub>600<\/sub>=6.0<\/th><\/tr><tr><td>1 mL<\/td><td>200 \u00b5L<\/td><td>600 \u00b5L<\/td><td>100 \u00b5L<\/td><\/tr><tr><td>5 mL<\/td><td>1 mL<\/td><td>3 mL<\/td><td>500 \u00b5L<\/td><\/tr><tr><td>10 mL<\/td><td>2 mL<\/td><td>6 mL<\/td><td>1 mL<\/td><\/tr><tr><td>50 mL<\/td><td>10 mL<\/td><td>30 mL<\/td><td>5 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>MagneGST\u2122 Protein Purification System(\u4ee5\u4e0bMagneGST\u2122)\u306f\u3001\u5927\u8178\u83cc\u3042\u308b\u3044\u306fin vitro\u7ffb\u8a33\u53cd\u5fdc\u5f8c\u3067\u767a\u73fe\u3055\u305b\u305f\u30b0\u30eb\u30bf\u30c1\u30aa\u30f3-S-\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30e9\u30fc\u30bc (GST) \u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[139],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1409"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1409"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1409\/revisions"}],"predecessor-version":[{"id":1896,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1409\/revisions\/1896"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1409"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1409"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1409"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}