{"id":1468,"date":"2020-12-03T15:47:13","date_gmt":"2020-12-03T06:47:13","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1468"},"modified":"2020-12-22T14:39:02","modified_gmt":"2020-12-22T05:39:02","slug":"q248","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/proteomics\/protein-tag\/halotag\/halotag-technology\/q248","title":{"rendered":"HaloTag\u00ae\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306b\u306f\u3069\u306e\u3088\u3046\u306a\u3082\u306e\u304c\u3042\u308a\u307e\u3059\u304b\uff1f"},"content":{"rendered":"\n

HaloTag\u00ae<\/sup>\u00a07\u767a\u73fe\u7528\u30d9\u30af\u30bf\u30fc\u3068\u3057\u3066\u4e0b\u8a18\u30d9\u30af\u30bf\u30fc\u3092\u7528\u610f\u3057\u3066\u3044\u307e\u3059(\u88681)\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u88681 HaloTag\u00ae<\/sup> 7\u767a\u73fe\u7528\u30d9\u30af\u30bf\u30fc<\/p>\n\n\n\n

\u6ce8) HaloTag\u00ae<\/sup>\u00a07\u767a\u73fe\u7528\u30d9\u30af\u30bf\u30fc\u306fFlexi\u00ae<\/sup>\u00a0Vector System\u306e\u30d9\u30af\u30bf\u30fc\u3067\u3042\u308b\u305f\u3081\u3001\u85ac\u5264\u8010\u6027\u907a\u4f1d\u5b50\u3068\u3057\u3066Ampr\u3092\u6301\u3064\u30d9\u30af\u30bf\u30fc(\u672b\u5c3e\u304cA)\u3068Kanr\u3092\u6301\u3064\u30d9\u30af\u30bf\u30fc(\u672b\u5c3e\u304cK)\u304c\u7528\u610f\u3055\u308c\u3066\u3044\u307e\u3059\u3002Flexi\u00ae<\/sup>\u00a0Vector System\u306b\u3064\u3044\u3066\u306f\u3053\u3061\u3089\u3002<\/a>

\u57fa\u672c\u30d9\u30af\u30bf\u30fc<\/strong>
\u54fa\u4e73\u52d5\u7269\u767a\u73fe\u7528\u306epFC14A\/K(C\u672b\u30bf\u30b0)\u3068pFN21A\/K(N\u672b\u30bf\u30b0)\u3001\u5927\u8178\u83cc\u767a\u73fe\u7528\u306epFN18A\/K\u3001\u7121\u7d30\u80de\u767a\u73fe\u7528\u306epFN19A\/K\u3067\u3059\u3002

\u9078\u629e\u30ac\u30a4\u30c9
http:\/\/www.promega.com\/pnotes\/100\/16620_13\/16620_13.pdf<\/a>

CMV Deletion\u30d9\u30af\u30bf\u30fc<\/strong>
pFC15\uff5e17A\/K\u3001pFN22\uff5e24A\/K\u306f\u3001CMV\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3092\u6539\u5909\u3057\u3001\u767a\u73fe\u91cf\u304c\u4f4e\u304f\u306a\u308b\u3088\u3046\u306b\u8a2d\u8a08\u3055\u308c\u3066\u3044\u307e\u3059\u3002pFC17A\/K\u304a\u3088\u3073pFN24A\/K\u304c\u6700\u3082\u767a\u73fe\u91cf\u306e\u4f4e\u3044\u30d9\u30af\u30bf\u30fc\u306b\u306a\u308a\u307e\u3059\u3002
HaloTag\u00ae<\/sup>\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u91cf\u304c\u9ad8\u3059\u304e\u308b\u3068\u3001\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u304c\u3046\u307e\u304f\u884c\u304b\u306a\u3044\u5834\u5408\u3084\u3001HaloCHIP\u2122 System\u3067\u30a2\u30c3\u30bb\u30a4\u7d50\u679c\u304c\u5f71\u97ff\u3092\u53d7\u3051\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u5834\u5408\u306f\u3088\u308a\u767a\u73fe\u91cf\u306e\u4f4e\u3044\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3092\u9078\u629e\u3059\u308b\u3053\u3068\u3067\u3001\u30a2\u30c3\u30bb\u30a4\u7d50\u679c\u304c\u6539\u5584\u3055\u308c\u308b\u30b1\u30fc\u30b9\u304c\u3042\u308a\u307e\u3059\u3002

\u53c2\u8003\u8cc7\u6599
http:\/\/www.promega.com\/pnotes\/100\/16620_16\/16620_16.pdf<\/a>

\uff50HT2\u30d9\u30af\u30bf\u30fc:
pHT2\u30d9\u30af\u30bf\u30fc\u306f\u3001\u53e4\u3044\u30bf\u30a4\u30d7\u306eHaloTag\u3092\u767a\u73fe\u3059\u308b\u30d9\u30af\u30bf\u30fc\u3067\u3059\u3002pHT2 Vector\u306fN\u672b\u7aef\u3001C\u672b\u7aef\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u3069\u3061\u3089\u3082\u4f5c\u6210\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u304c\u3001\u30ea\u30f3\u30ab\u30fc\u914d\u5217\u304c\u77ed\u3044\u305f\u3081\u3001\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u3057\u3066\u767a\u73fe\u3055\u305b\u305f\u5834\u5408\u3001\u30ea\u30ac\u30f3\u30c9\u3068\u306e\u7d50\u5408\u304c\u4e0d\u5341\u5206\u3067\u3042\u3063\u305f\u308a\u3059\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002\u8ad6\u6587\u3067\u306e\u4f7f\u7528\u5b9f\u7e3e\u3082\u3042\u308b\u305f\u3081\u7d99\u7d9a\u3057\u3066\u8ca9\u58f2\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u65b0\u3057\u3044Flexi\u30bf\u30a4\u30d7\u306eHaloTag7\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306e\u4f7f\u7528\u3092\u304a\u52e7\u3081\u3057\u307e\u3059\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

HaloTag\u00ae\u00a07\u767a\u73fe\u7528\u30d9\u30af\u30bf\u30fc\u3068\u3057\u3066\u4e0b\u8a18\u30d9\u30af\u30bf\u30fc\u3092\u7528\u610f\u3057\u3066\u3044\u307e\u3059(\u88681)\u3002 \u88681 HaloTag\u00ae 7\u767a\u73fe\u7528\u30d9\u30af\u30bf\u30fc \u6ce8) HaloTag\u00ae\u00a07\u767a\u73fe\u7528\u30d9\u30af\u30bf\u30fc\u306fFlexi\u00ae\u00a0Vector System\u306e\u30d9\u30af … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[140],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1468"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1468"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1468\/revisions"}],"predecessor-version":[{"id":1908,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1468\/revisions\/1908"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1468"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1468"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1468"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}