{"id":149,"date":"2020-12-02T10:56:31","date_gmt":"2020-12-02T01:56:31","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=149"},"modified":"2021-02-15T17:15:15","modified_gmt":"2021-02-15T08:15:15","slug":"q48","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/amplification-quantification\/pcr-and-rt\/total\/pcr-info\/q48","title":{"rendered":"\u3069\u306e\u8010\u71b1\u6027DNA polymerase\u304c\u975e\u92f3\u578b\u4f9d\u5b58\u7684\u306b3\u2019\u672b\u7aef\u306bA\u3092\u4ed8\u52a0\u3057\u307e\u3059\u304b\u3002"},"content":{"rendered":"\n

3\u00b4\u21925\u00b4 exonuclease\u6d3b\u6027\u3092\u6b20\u3044\u305f\u8010\u71b1\u6027DNA Polymerase\u306f\u92f3\u578b\u306b\u4f9d\u5b58\u305b\u305a\u306b\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\uff08\u901a\u5e38\u306fdATP\uff09\u3092\u5897\u5e45\u7523\u7269\u306e3\u2019\u672b\u7aef\u306b\u4ed8\u52a0\u3057\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u9175\u7d20\u306b\u306f\u3001Taq<\/em> DNA Polymerase\u3001Tfl<\/em> DNA Polymerase\u3001Tth<\/em> DNA Polymerase\u304c\u542b\u307e\u308c\u307e\u3059 (\u8868 1)\u3002polymerase\u304c3\u00b4\u21925\u00b4 exonuclease proofreading\u6d3b\u6027\u3092\u6301\u3064\u5834\u5408\u3001 \u3053\u306e\u6d3b\u6027\u306b\u3088\u3063\u3066\u92f3\u578b\u306b\u4f9d\u5b58\u3057\u306a\u3044\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u306f\u9664\u304b\u308c\u3001\u5e73\u6ed1\u672b\u7aef\u3092\u6709\u3059\u308b\u7523\u7269\u3068\u306a\u308a\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u9175\u7d20\u306b\u306fTli<\/em> DNA Polymerase\u3001Pfu<\/em> DNA Polymerase\u306a\u3069\u304c\u542b\u307e\u308c\u307e\u3059\u3002\uff08PN71-Q&A)<\/p>\n\n\n\n

\u88681\uff0e\u8010\u71b1\u6027DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u7279\u5fb4<\/strong><\/p>\n\n\n\n

\u7279\u5fb4<\/th>Taq<\/em> DNA Polymerase<\/th>Tfl <\/em>DNA Polymerase<\/th>Tth<\/em> DNA Polymerase<\/th>Tli <\/em>DNA Polymerase<\/th>Pfu<\/em> DNA Polymerase<\/th><\/tr><\/thead>
\u7531\u6765<\/td>Thermus Aquaticus<\/td>Thermus flavis<\/td>Thermus thermophilus HB-8<\/td>Thermococcus litoralis<\/td>Pyrococcus furiosus<\/td><\/tr>
\u5206\u5b50\u91cf<\/td>94kDa<\/td>94kDa<\/td>94kDa<\/td>90kDa<\/td>92kDa<\/td><\/tr>
\u534a\u6e1b\u671f\uff0895\u2103\uff09<\/td>40\u5206<\/td>40\u5206<\/td>20\u5206<\/td>400\u5206<\/td>\u203b<\/td><\/tr>
\u4f38\u9577\u7387\uff08nt\/sec. @72\u2103\uff09<\/td>60\uff5e150<\/td>\u203b\u203b<\/td>25<\/td>67<\/td>\u203b<\/td><\/tr>
\u9006\u8ee2\u5199\u6d3b\u6027<\/td>\u4f4e\u30ec\u30d9\u30eb<\/td>\u4f4e\u30ec\u30d9\u30eb<\/td>\u3007
\uff08Mn2+\u4f9d\u5b58\u6027\uff09<\/td>		\u00d7<\/td>\u203b<\/td><\/tr>
\u30b5\u30a4\u30af\u30eb\u534a\u6e1b\u671f<\/td>50\u30b5\u30a4\u30af\u30eb<\/td>50\u30b5\u30a4\u30af\u30eb<\/td>25\u30b5\u30a4\u30af\u30eb<\/td>500\u30b5\u30a4\u30af\u30eb<\/td>\u203b<\/td><\/tr>
3’A\u306e\u4ed8\u52a0<\/td>\u3007<\/td>\u3007<\/td>\u3007<\/td>\u00d7<\/td>\u00d7<\/td><\/tr>
5’\u21923′ \u30a8\u30af\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027<\/td>\u3007<\/td>\u3007<\/td>\u3007<\/td>\u00d7<\/td>\u00d7<\/td><\/tr>
3\u2192’5′ \u30a8\u30af\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027<\/td>\u00d7<\/td>\u00d7<\/td>\u00d7<\/td>\u3007<\/td>\u3007<\/td><\/tr>
\u203b\u30c7\u30fc\u30bf\u30fc\u7121\u3000\u3000\u203b\u203b\u89e3\u6790\u4e2d<\/td><\/tr><\/tbody><\/table><\/figure>\n","protected":false},"excerpt":{"rendered":"

3\u00b4\u21925\u00b4 exonuclease\u6d3b\u6027\u3092\u6b20\u3044\u305f\u8010\u71b1\u6027DNA Polymerase\u306f\u92f3\u578b\u306b\u4f9d\u5b58\u305b\u305a\u306b\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\uff08\u901a\u5e38\u306fdATP\uff09\u3092\u5897\u5e45\u7523\u7269\u306e3\u2019\u672b\u7aef\u306b\u4ed8\u52a0\u3057\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u9175\u7d20\u306b\u306f\u3001Taq DNA Polyme … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[56],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/149"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=149"}],"version-history":[{"count":17,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/149\/revisions"}],"predecessor-version":[{"id":1844,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/149\/revisions\/1844"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=149"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=149"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=149"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}