{"id":1502,"date":"2020-12-03T15:52:23","date_gmt":"2020-12-03T06:52:23","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1502"},"modified":"2020-12-17T09:44:17","modified_gmt":"2020-12-17T00:44:17","slug":"q257","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/proteomics\/protein-tag\/halotag\/halotag-protein\/q257","title":{"rendered":"HaloTag\u00ae\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u5b50\u91cf\u306f\u300133 kDa\u3068\u306e\u3053\u3068\u3067\u3059\u304c\u3001HaloTag\u00ae\u3068\u878d\u5408\u3055\u305b\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6d3b\u6027\u306f\u4fdd\u6301\u3055\u308c\u3066\u3044\u308b\u306e\u3067\u3059\u304b\uff1f"},"content":{"rendered":"\n<p>\u30d7\u30ed\u30e1\u30ac\u3067\u306f\u3001\u4ee5\u4e0b\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3068HaloTag\u306e\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u6d3b\u6027\u304c\u4fdd\u6301\u3055\u308c\u3066\u3044\u308b\u306e\u3092\u78ba\u8a8d\u3057\u3066\u3044\u307e\u3059\u3002<br>(\u6d3b\u6027\u3092\u78ba\u8a8d\u3057\u3066\u3044\u308b\u30bf\u30f3\u30d1\u30af\u8cea\uff1aRenilla Luciferase, p65, beta1 integrin, HERG channel (potassium channel), GPI, Kv4 (potassium channel)<br>\u306a\u304a\u3001\u30d7\u30ed\u30e1\u30ac\u3067\u306f\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u4f5c\u6210\u6642\u306b\u3001(\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u306b)\u30ea\u30f3\u30ab\u30fc\u306e\u633f\u5165\u3092\u63a8\u5968\u3044\u305f\u3057\u307e\u3059\u3002\u878d\u5408\u30d1\u30fc\u30c8\u30ca\u30fc\u306e\u30b5\u30a4\u30ba\u304c\u5927\u304d\u3044\u5834\u5408\u306a\u3069\u306b\u3001\u7acb\u4f53\u969c\u5bb3\u306b\u3088\u3063\u3066HaloTag<sup>\u00ae<\/sup>\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u30ea\u30ac\u30f3\u30c9\u306e\u7d50\u5408\u304c\u963b\u5bb3\u3055\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u30ea\u30f3\u30ab\u30fc\u3092\u633f\u5165\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3001\u3053\u306e\u3088\u3046\u306a\u7acb\u4f53\u969c\u5bb3\u3092\u56de\u907f\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<br>\u5168\u3066\u306eHaloTag<sup>\u00ae<\/sup>&nbsp;7\u30d9\u30af\u30bf\u30fc\u306b\u306f\u3042\u3089\u304b\u3058\u3081TEV\u5207\u65ad\u914d\u5217\u3092\u542b\u3080\u30ea\u30f3\u30ab\u30fc\u914d\u5217\u304c\u633f\u5165\u3055\u308c\u3066\u3044\u308b\u306e\u3067\u3001\u6539\u3081\u3066\u30ea\u30f3\u30ab\u30fc\u3092\u8a2d\u8a08\u30fb\u633f\u5165\u3059\u308b\u5fc5\u8981\u306f\u3042\u308a\u307e\u305b\u3093\u3002<br>HaloTag<sup>\u00ae<\/sup>&nbsp;7\u30d9\u30af\u30bf\u30fc\u306e\u30ea\u30f3\u30ab\u30fc\u4ee5\u5916\u306e\u914d\u5217\u3092\u4f7f\u3046\u5fc5\u8981\u304c\u3042\u308b\u5834\u5408\u306f\u3001\u4e0b\u8a18\u306e\u30dd\u30a4\u30f3\u30c8\u306b\u6ce8\u610f\u3057\u3066\u65b0\u3057\u304f\u30ea\u30f3\u30ab\u30fc\u3092\u8a2d\u8a08\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<ul><li>\u4e00\u822c\u7684\u306a\u30ea\u30f3\u30ab\u30fc\u3068\u3057\u3066\u3001\u30b0\u30ea\u30b7\u30f3(Gly)-\u30bb\u30ea\u30f3(Ser)\u30ea\u30d4\u30fc\u30c8\u304c\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u30a2\u30df\u30ce\u9178\u5206\u5b50\u306f\u5c0f\u3055\u3044\u305f\u3081\u3001\u30ea\u30f3\u30ab\u30fc\u306b\u5165\u308c\u308b\u3053\u3068\u304c\u63a8\u5968\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u4ee3\u8868\u7684\u306a\u3082\u306e\u3068\u3057\u3066Ser-Gly4\u30ea\u30d4\u30fc\u30c8\u304c\u4f7f\u7528\u3055\u308c\u3066\u3044\u307e\u3059(Ser-Gly-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Gly-Ser\u2026)\u3002\u878d\u5408\u30d1\u30fc\u30c8\u30ca\u30fc\u306b\u3088\u3063\u3066\u306f\u30ea\u30f3\u30ab\u30fc\u306e\u6700\u9069\u5316\u304c\u5fc5\u8981\u3067\u3059\u3002<\/li><li>\u30d7\u30ed\u30ea\u30f3\u3001\u307e\u305f\u306f\u5927\u304d\u3044\u30a2\u30df\u30ce\u9178\u3084\u30c1\u30e3\u30fc\u30b8\u3057\u305f\u30a2\u30df\u30ce\u9178\u306f\u3001\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u69cb\u9020\u306b\u60aa\u5f71\u97ff\u3092\u4e0e\u3048 (\u30d7\u30ed\u30ea\u30f3\u306f\u30da\u30d7\u30c1\u30c9\u9396\u3092\u66f2\u3052\u308b)\u3001\u307e\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6027\u8cea\u3092\u5909\u3048\u308b\u305f\u3081\u3001\u30ea\u30f3\u30ab\u30fc\u914d\u5217\u306b\u306f\u5165\u308c\u306a\u3044\u3088\u3046\u306b\u3057\u307e\u3059\u3002<\/li><li>HaloTag<sup>\u00ae<\/sup>\u30bf\u30f3\u30d1\u30af\u8cea\u7528\u306e\u30ea\u30f3\u30ab\u30fc\u306e\u9577\u3055\u306f\u300115\uff5e21\u30a2\u30df\u30ce\u9178\u3092\u63a8\u5968\u3057\u307e\u3059\u3002\u878d\u5408\u30d1\u30fc\u30c8\u30ca\u30fc\u306b\u3088\u3063\u3066\u306f\u3001\u3088\u308a\u9577\u3044\u30ea\u30f3\u30ab\u30fc\u304c\u5fc5\u8981\u306b\u306a\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/li><li>\u30b5\u30a4\u30ba\u306e\u5c0f\u3055\u3044\u30bf\u30b0(GST\u3001FLAG\u2122\u3001\u5c40\u5728\u30b7\u30b0\u30ca\u30eb\u914d\u5217)\u3068HaloTag<sup>\u00ae<\/sup>&nbsp;\u306e\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u306f\u30ea\u30f3\u30ab\u30fc\u306f\u5fc5\u8981\u3042\u308a\u307e\u305b\u3093\u3002\u3053\u308c\u3089\u306e\u914d\u5217\u306fHaloTag<sup>\u00ae<\/sup>\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u96a3\u63a5\u3055\u305b\u3066\u8a2d\u8a08\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/li><\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u30d7\u30ed\u30e1\u30ac\u3067\u306f\u3001\u4ee5\u4e0b\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3068HaloTag\u306e\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u6d3b\u6027\u304c\u4fdd\u6301\u3055\u308c\u3066\u3044\u308b\u306e\u3092\u78ba\u8a8d\u3057\u3066\u3044\u307e\u3059\u3002(\u6d3b\u6027\u3092\u78ba\u8a8d\u3057\u3066\u3044\u308b\u30bf\u30f3\u30d1\u30af\u8cea\uff1aRenilla Luciferase, p65, beta1 integrin, H &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[141],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1502"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1502"}],"version-history":[{"count":1,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1502\/revisions"}],"predecessor-version":[{"id":1503,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1502\/revisions\/1503"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1502"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1502"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1502"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}