{"id":1516,"date":"2020-12-03T15:56:06","date_gmt":"2020-12-03T06:56:06","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1516"},"modified":"2020-12-22T14:48:50","modified_gmt":"2020-12-22T05:48:50","slug":"q264","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/proteomics\/protein-tag\/halotag\/halotag-ligand\/q264","title":{"rendered":"HaloTag\u00ae\u30ea\u30ac\u30f3\u30c9\u306b\u306f\u3069\u306e\u3088\u3046\u306a\u3082\u306e\u304c\u3042\u308a\u307e\u3059\u304b\uff1f"},"content":{"rendered":"\n<p>\u30ea\u30ac\u30f3\u30c9\u306b\u306f\u3001\u2460\u86cd\u5149\u6a19\u8b58\u7528\u30ea\u30ac\u30f3\u30c9\u3001\u2461\u30d3\u30aa\u30c1\u30f3\u6a19\u8b58\u30ea\u30ac\u30f3\u30c9\u3001\u2462\u30bf\u30f3\u30d1\u30af\u8cea\u7cbe\u88fd\u3001\u30d7\u30eb\u30c0\u30a6\u30f3\u7528\u30ea\u30ac\u30f3\u30c9\u3001\u2463\u672a\u6a19\u8b58\u30ea\u30ac\u30f3\u30c9\u304c\u3042\u308a\u307e\u3059\u3002<br><br>\u2460 \u86cd\u5149\u6a19\u8b58\u30ea\u30ac\u30f3\u30c9\u306b\u306f\u3001\u7d30\u80de\u819c\u900f\u904e\u6027\u30ea\u30ac\u30f3\u30c9\u3068\u3001\u975e\u900f\u904e\u6027\u306e\u30ea\u30ac\u30f3\u30c9\u304c\u3042\u308a\u307e\u3059\u3002\u7d30\u80de\u819c\u975e\u900f\u904e\u6027\u306e\u30ea\u30ac\u30f3\u30c9\u306f\u3001\u7d30\u80de\u8868\u9762\u306b\u767a\u73fe\u3059\u308bHaloTag<sup>\u00ae<\/sup>\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u7d50\u5408\u3059\u308b\u3053\u3068\u304c\u51fa\u6765\u307e\u3059\u304c\u3001\u7d30\u80de\u5185\u306eHaloTag<sup>\u00ae<\/sup>\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u306f\u7d50\u5408\u3067\u304d\u307e\u305b\u3093\u3002\u7d30\u80de\u819c\u304b\u3089\u7d30\u80de\u8cea\u3078\u306e\u79fb\u884c\u3092\u89b3\u5bdf\u3059\u308b\u5834\u5408\u306b\u6709\u7528\u3067\u3059\u3002\u3000<br><br>\u7d30\u80de\u819c\u900f\u904e\u6027\u306e\u30ea\u30ac\u30f3\u30c9\u306e\u3046\u3061\u3001Oregon Green<sup>\u00ae<\/sup>&nbsp;Ligand\u3068diAcFAM Ligand\u306f\u3001\u7d30\u80de\u5185\u306e\u30a8\u30b9\u30c6\u30e9\u30fc\u30bc\u306b\u3088\u308a\u4fee\u98fe\u57fa\u304c\u52a0\u6c34\u5206\u89e3\u3055\u308c\u308b\u3053\u3068\u306b\u3088\u308a\u86cd\u5149\u3092\u767a\u3057\u307e\u3059\u3002Oregon Green<sup>\u00ae<\/sup>\u306e\u65b9\u304c\u3001\u892a\u8272\u304c\u5c11\u306a\u3044\u306e\u304c\u7279\u9577\u3067\u3059\u3002<br><br>No Wash\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u7528\u306b\u958b\u767a\u3057\u305fDirect Ligand\u3082\u7d30\u80de\u819c\u3092\u900f\u904e\u3057\u3001\u8d64\uff08TMR\uff09\u3001\u7dd1\uff08R110\uff09\u306e\u86cd\u5149\u3067\u89b3\u5bdf\u53ef\u80fd\u3067\u3059\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"442\" height=\"877\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/HaloTag_03.gif\" alt=\"\" class=\"wp-image-1914\"\/><\/figure>\n\n\n\n<p>\u2461 \u30d3\u30aa\u30c1\u30f3\u6a19\u8b58\u7528\uff08Biotin\u3001PEG-Biotin\uff09\u30ea\u30ac\u30f3\u30c9\u306f\u3001HaloTag\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u30d3\u30aa\u30c1\u30f3\u6a19\u8b58\u3092\u3059\u308b\u4e8b\u304c\u3067\u304d\u307e\u3059\u3002PEG-Biotin\u30ea\u30ac\u30f3\u30c9\u306f\u7d30\u80de\u819c\u900f\u904e\u6027\u304cBiotin \u30ea\u30ac\u30f3\u30c9\u306b\u6bd4\u3079\u3066\u4f4e\u3044\u305f\u3081\u3001\u7cbe\u88fd\u64cd\u4f5c\u306f\u7d30\u80de\u3092\u6eb6\u89e3\u3057\u3066\u30e9\u30a4\u30bb\u30fc\u30c8\u306b\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u304c\u5bfe\u8c61\u306b\u306a\u308a\u307e\u3059\u3002\u751f\u304d\u305f\u7d30\u80de\u3067\u6a19\u8b58\/\u7cbe\u88fd\u3059\u308b\u5834\u5408\u306fBiotin\u30ea\u30ac\u30f3\u30c9\u3092\u63a8\u5968\u3057\u307e\u3059\u3002Pull-down Assay\u306a\u3069\u8907\u5408\u4f53\u3068\u3057\u3066HaloTag\u3092\u7cbe\u88fd\u3059\u308b\u969b\u306b\u306fPEG\u30ea\u30d4\u30fc\u30c8\u304c\u30b9\u30da\u30fc\u30b5\u30fc\u3068\u3057\u3066\u6a5f\u80fd\u3059\u308b\u305f\u3081\u3001PEG-Biotin \u30ea\u30ac\u30f3\u30c9\u304c\u52b9\u679c\u7684\u3067\u3059\u3002<\/p>\n\n\n\n<p>HaloTag<sup>\u00ae<\/sup>&nbsp;Biotin Ligand<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"288\" height=\"65\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/HaloTag_04.gif\" alt=\"\" class=\"wp-image-1916\"\/><\/figure>\n\n\n\n<p>HaloTag<sup>\u00ae<\/sup>&nbsp;PEG-Biotin Ligand<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"464\" height=\"80\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/HaloTag_05.gif\" alt=\"\" class=\"wp-image-1917\"\/><\/figure>\n\n\n\n<p>\u2462\u3000\u30bf\u30f3\u30d1\u30af\u8cea\u7cbe\u88fd\u3001\u30d7\u30eb\u30c0\u30a6\u30f3\u7528\u30ea\u30ac\u30f3\u30c9\u3068\u3057\u3066\u3001HaloLink\u2122 Resin, HaloLink\u2122 Magnetic Beads\u3092\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u30ea\u30ac\u30f3\u30c9\u3067HaloTag\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u30d3\u30fc\u30ba\u306b\u56fa\u5b9a\u5316\u3059\u308b\u3053\u3068\u304c\u51fa\u6765\u3001\u307e\u305f\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u306f\u3001TEV\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\uff08ProTEV;\u30ab\u30bf\u30ed\u30b0\u756a\u53f7V6051\uff09\u3092\u7528\u3044\u3066\u5206\u96e2\u3001\u7cbe\u88fd\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"461\" height=\"277\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/pic_halotag_04.jpg\" alt=\"\" class=\"wp-image-1918\"\/><\/figure>\n\n\n\n<p>4.\u3000\u672a\u6a19\u8b58\u30ea\u30ac\u30f3\u30c9\u306b\u306f\u3001Amine Ligand, Sccinimidyl Ester Ligand, Thiol Ligand, Iodoacetamide Ligand\u3092\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002\u30ea\u30ac\u30f3\u30c9\u306e\u9577\u3055\u304c\u7570\u306a\u308bO2\u3001O4\u306e\u4e8c\u7a2e\u985e\u306e\u30bf\u30a4\u30d7\u3092\u7528\u610f\u3057\u3066\u3044\u308b\u3082\u306e\u3082\u3042\u308a\u307e\u3059\u3002\u4fee\u98fe\u30ea\u30ac\u30f3\u30c9\u3092\u4f5c\u6210\u3057\u3001\u8272\u3005\u306a\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u6d3b\u7528\u3057\u3066\u304f\u3060\u3055\u3044\u3002OligoDNA\u306e\u4fee\u98fe\u306a\u3069\u3082\u53ef\u80fd\u3067\u3059\u3002 \u8a73\u7d30\u306a\u60c5\u5831\u306f\u3001\u4e0b\u8a18\u306e\u82f1\u6587\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u3092\u53c2\u8003\u306b\u3057\u3066\u304f\u3060\u3055\u3044\u3002<br><a href=\"http:\/\/www.promega.com\/catalog\/catalogproducts.aspx?categoryname=productleaf_1675&amp;ckt=1\" target=\"_blank\" rel=\"noreferrer noopener\">http:\/\/www.promega.com\/catalog\/catalogproducts.aspx?categoryname=productleaf_1675&amp;ckt=1<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30ea\u30ac\u30f3\u30c9\u306b\u306f\u3001\u2460\u86cd\u5149\u6a19\u8b58\u7528\u30ea\u30ac\u30f3\u30c9\u3001\u2461\u30d3\u30aa\u30c1\u30f3\u6a19\u8b58\u30ea\u30ac\u30f3\u30c9\u3001\u2462\u30bf\u30f3\u30d1\u30af\u8cea\u7cbe\u88fd\u3001\u30d7\u30eb\u30c0\u30a6\u30f3\u7528\u30ea\u30ac\u30f3\u30c9\u3001\u2463\u672a\u6a19\u8b58\u30ea\u30ac\u30f3\u30c9\u304c\u3042\u308a\u307e\u3059\u3002 \u2460 \u86cd\u5149\u6a19\u8b58\u30ea\u30ac\u30f3\u30c9\u306b\u306f\u3001\u7d30\u80de\u819c\u900f\u904e\u6027\u30ea\u30ac\u30f3\u30c9\u3068\u3001\u975e\u900f\u904e\u6027\u306e\u30ea\u30ac\u30f3\u30c9\u304c\u3042\u308a\u307e\u3059\u3002\u7d30\u80de\u819c\u975e\u900f &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[142],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1516"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1516"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1516\/revisions"}],"predecessor-version":[{"id":1919,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1516\/revisions\/1919"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1516"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1516"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1516"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}