{"id":1536,"date":"2020-12-03T15:59:33","date_gmt":"2020-12-03T06:59:33","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1536"},"modified":"2021-01-22T14:21:08","modified_gmt":"2021-01-22T05:21:08","slug":"q332","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/proteomics\/invitro-transcription-translation\/modification\/transcend-non-radioactive-translation-detection\/q332","title":{"rendered":"\u5927\u8178\u83cc\u306e\u7121\u7d30\u80de\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u7cfb\uff08E. Coli S30 Extract\uff09\u306bTranscend\u2122\u3092\u7528\u3044\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u3059\u304b\uff1f"},"content":{"rendered":"\n

E. Coli<\/em> S30 Extract\u3068Transcend\u2122\u3092\u7d44\u307f\u5408\u308f\u305b\u3066\u4f7f\u7528\u3059\u308b\u3053\u3068\u306f\u53ef\u80fd\u3067\u3059\u3002S30 System\u3068Transcend\u2122\u3092\u4f7f\u3063\u305f\u5408\u6210\u3057\u305f\u53cd\u5fdc\u6eb6\u6db25\u00b5L\u306b\u30a2\u30bb\u30c8\u30f320\u00b5L\u3092\u6dfb\u52a0\u305715\u5206\u9593\u9060\u5fc3\u3057\u3066\u6c88\u6bbf\u3055\u305b\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u3042\u3068\u3067\u300120\u00b5L\u306eLoading Buffer\u306b\u61f8\u6fc1\u3057SDS-PAGE\u3092\u5b9f\u65bd\u3057\u3066\u3044\u307e\u3059\u3002\u53cd\u5fdc\u6eb6\u6db2\u3092\u76f4\u63a5\u6dfb\u52a0\u3059\u308b\u5834\u5408\u306f\u3001\u53cd\u5fdc5\uff5e8\u00b5L\u3092\u4f7f\u7528\u3057\u3066\u304f\u3060\u3055\u3044(SDS-PAGE\u306b\u30a2\u30d7\u30e9\u30a4\u3059\u308b\u91cf\u306f10\u00b5L\u3067\u3059)\u3002<\/p>\n\n\n\n

Transcend\u2122\u306e\u88fd\u54c1\u30d7\u30ed\u30c8\u30b3\u30eb\u306b\u8a18\u8f09\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u5927\u8178\u83cc\u306e\u7ffb\u8a33\u7cfb\u3092\u7528\u3044\u308b\u5834\u5408\u306f\u3001\u30a2\u30df\u30ce\u9178\u306e\u30ea\u30b8\u30f3\u30de\u30a4\u30ca\u30b9\u3092\u4f7f\u7528\u3059\u308b\u3068\u52b9\u679c\u7684\u3067\u3059\u3002<\/p>\n\n\n\n

\u307e\u305f\u3001\u4ee5\u4e0b\u306e\u3053\u3068\u306b\u6ce8\u610f\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

  1. PVDF\u30e1\u30f3\u30d6\u30ec\u30f3\u306b\u8ee2\u5199\u5f8c\u306eBlocking\u306f\u30010.5%Tween\/TBS\u30671\uff5e2\u6642\u9593\u5b9f\u65bd\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>
  2. SDS-PAGE\u30b2\u30eb\u306b\u30a2\u30d7\u30e9\u30a4\u3059\u308b\u969b\u306f\u30011\u00b5L\u306e\u53cd\u5fdc\u6eb6\u6db2\u3092\u4f7f\u7528\u3057\u3066\u304f\u3060\u3055\u3044\u3002(\u3053\u308c\u4ee5\u4e0a\u6dfb\u52a0\u3057\u3066\u3082\u9ad8\u3044\u52b9\u679c\u306f\u5f97\u3089\u308c\u307e\u305b\u3093\u3002)<\/li>
  3. 50\u00b5L\u306eE. Coli<\/em> S30\u53cd\u5fdc\u6eb6\u6db2\u306b\u5bfe\u3057\u3001\u6700\u5927\u30672\uff5e3\u00b5L\u306eTranscend\u2122\u3092\u4f7f\u7528\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li><\/ol>\n","protected":false},"excerpt":{"rendered":"

    E. Coli S30 Extract\u3068Transcend\u2122\u3092\u7d44\u307f\u5408\u308f\u305b\u3066\u4f7f\u7528\u3059\u308b\u3053\u3068\u306f\u53ef\u80fd\u3067\u3059\u3002S30 System\u3068Transcend\u2122\u3092\u4f7f\u3063\u305f\u5408\u6210\u3057\u305f\u53cd\u5fdc\u6eb6\u6db25\u00b5L\u306b\u30a2\u30bb\u30c8\u30f320\u00b5L\u3092\u6dfb\u52a0\u305715\u5206\u9593\u9060\u5fc3\u3057 … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[156],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1536"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1536"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1536\/revisions"}],"predecessor-version":[{"id":2431,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1536\/revisions\/2431"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1536"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1536"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1536"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}