{"id":1589,"date":"2020-12-03T16:25:07","date_gmt":"2020-12-03T07:25:07","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1589"},"modified":"2020-12-22T16:34:15","modified_gmt":"2020-12-22T07:34:15","slug":"q294","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/proteomics\/invitro-transcription-translation\/kits\/tnt-system\/q294","title":{"rendered":"TNT\u00ae System \u306e\u3046\u3061\u3001RRL\u3068WGE\u306e\u3069\u3061\u3089\u3092\u9078\u629e\u3059\u308c\u3070\u3088\u3044\u3067\u3059\u304b\uff1f"},"content":{"rendered":"\n

\u5408\u6210\u3057\u305f\u3044\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7531\u6765\u3001\u92f3\u578bDNA\u306e\u5f62\u614b\uff08\u74b0\u72b6DNA\u307e\u305f\u306f\u76f4\u9396DNA\uff09\u3068\u92f3\u578bDNA\u306b\u542b\u307e\u308c\u308b\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u914d\u5217 (T7, SP6, T3) \u304b\u3089\u6700\u9069\u306aTNT\u00ae<\/sup> System\u3092\u6c7a\u5b9a\u3057\u3066\u304f\u3060\u3055\u3044\uff08\u88681\uff5e\u88683\uff09\u3002<\/p>\n\n\n\n

\u88681 in vitro \u7ffb\u8a33\u30b7\u30b9\u30c6\u30e0\u306e\u92f3\u578b\u306e\u7531\u6765\u306b\u3088\u308b\u9078\u629e\u30ac\u30a4\u30c9<\/p>\n\n\n\n

\"\"<\/figure><\/div>\n\n\n\n

\u88682\u3000\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u3088\u308bin vitro \u30bf\u30f3\u30d1\u30af\u8cea\u7ffb\u8a33\u30b7\u30b9\u30c6\u30e0\u9078\u629e\u30ac\u30a4\u30c9<\/p>\n\n\n\n

\"\"<\/figure><\/div>\n\n\n\n

\u88683\u3000TNT System\u3068\u92f3\u578bDNA\u3001\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306e\u7d44\u307f\u5408\u308f\u305b<\/p>\n\n\n\n

TNT\u00ae<\/sup> System
\u88fd\u54c1\u540d<\/th>		\u30e9\u30a4
\u30bb\u30fc\u30c8<\/th>		\u92f3\u578bDNA<\/th>\u30de\u30b9\u30bf
\u30fc\u30df\u30c3
\u30af\u30b9*<\/th>		\u30b3\u30f3\u30c8
\u30ed\u30fc\u30eb
DNA<\/th>		\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc<\/th><\/tr>
TNT\u00ae<\/sup> T7 Quick
for PCR DNA<\/td>		RRL<\/td>\u74b0\u72b6DNA (\u53ef\u80fd)
\u76f4\u9396DNA (\u63a8\u5968)<\/span><\/strong><\/td>		\u25cb<\/td>\u4e0d\u542b<\/td>T7 Promoter<\/td><\/tr>
TNT\u00ae<\/sup> Quick Coupled
Transcription \/
Translation System<\/td>		RRL<\/td>\u74b0\u72b6DNA (\u63a8\u5968)<\/span><\/strong>
\u76f4\u9396DNA (\u53ef\u80fd)<\/td>		\u25cb<\/td>\u4e0d\u542b<\/td>T7 Promoter
SP6 Promoter<\/td><\/tr>
TNT\u00ae<\/sup> Coupled
Rabbit Reticulocyte
Lysate System<\/td>		RRL<\/td>\u74b0\u72b6DNA (\u63a8\u5968)<\/span><\/strong>
\u76f4\u9396DNA (\u53ef\u80fd)<\/td>		\u00d7<\/td>\u542b<\/td>T7 Promoter
T3 Promoter
SP6 Promoter<\/td><\/tr>
TNT\u00ae<\/sup> Coupled
Wheat Germ
Extract System<\/td>		WGE<\/td>\u74b0\u72b6DNA (\u8a31\u5bb9)
\u76f4\u9396DNA (\u8a31\u5bb9)<\/strong><\/td>		\u00d7<\/td>\u542b<\/td>T7 Promoter
T3 Promoter
SP6 Promoter<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

*:\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u3068\u306f\u3001RRL\u3068\u53cd\u5fdcBuffer\u3001\u30a2\u30df\u30ce\u9178Mixture (minus \u30e1\u30c1\u30aa\u30cb\u30f3)\u306e\u6df7\u5408\u6eb6\u6db2\u306b\u306a\u3063\u3066\u3044\u308b\u305f\u3081\u3001\u8a66\u85ac\u8abf\u88fd\u306e\u969b\u306b\u5404\u53cd\u5fdc\u7d44\u6210\u6eb6\u6db2\u3092\u5206\u6ce8\u3059\u308b\u30b9\u30c6\u30c3\u30d7\u304c\u7c21\u7565\u5316\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n

\u4e00\u822c\u7684\u306b\u306f\u3001\u54fa\u4e73\u985e\u7531\u6765\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u3092\u76ee\u7684\u3068\u3057\u305f\u5834\u5408\u306b\u3001RRL\u3092\u4f7f\u7528\u3057\u3001\u690d\u7269\u7531\u6765\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u5408\u6210\u306a\u3069\u306b\u306f\u3001WGE\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5408\u6210\u3001\u7ffb\u8a33\u5f8c\u4fee\u98fe\uff08\u7cd6\u9396\u4ed8\u52a0\u3001\u30ea\u30f3\u9178\u5316\u306a\u3069\uff09\u5fc5\u8981\u3068\u3059\u308b\u767a\u73fe\u7cfb\u306b\u3064\u3044\u3066\u306f\u3001RRL\u304c\u7814\u7a76\u5831\u544a\u306a\u3069\u304b\u3089\u6709\u52b9\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n

\u305f\u3060\u3057\u3001\u4ee5\u4e0b\u306b\u793a\u3059\u92f3\u578bDNA\u3068\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3001TNT\u00ae<\/sup> System\u306e\u7d44\u307f\u5408\u308f\u305b\u306f\u907f\u3051\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

1) \u76f4\u9396DNA\u3001 SP6 promoter\u3001RRL \u307e\u305f\u306fWGE<\/p>\n\n\n\n

2) \u74b0\u72b6DNA \u3001T7 promoter\u3001 WGE<\/p>\n","protected":false},"excerpt":{"rendered":"

\u5408\u6210\u3057\u305f\u3044\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7531\u6765\u3001\u92f3\u578bDNA\u306e\u5f62\u614b\uff08\u74b0\u72b6DNA\u307e\u305f\u306f\u76f4\u9396DNA\uff09\u3068\u92f3\u578bDNA\u306b\u542b\u307e\u308c\u308b\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u914d\u5217 (T7, SP6, T3) \u304b\u3089\u6700\u9069\u306aTNT\u00ae System\u3092\u6c7a\u5b9a\u3057\u3066\u304f\u3060\u3055\u3044\uff08\u88681\uff5e\u88683\uff09\u3002 … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[151],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1589"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1589"}],"version-history":[{"count":16,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1589\/revisions"}],"predecessor-version":[{"id":1959,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1589\/revisions\/1959"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1589"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1589"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1589"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}