{"id":159,"date":"2020-12-02T11:01:31","date_gmt":"2020-12-02T02:01:31","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=159"},"modified":"2021-03-01T10:38:34","modified_gmt":"2021-03-01T01:38:34","slug":"q51","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/amplification-quantification\/pcr-and-rt\/gotaq-total\/basic-info\/q51","title":{"rendered":"GoTaq\u00ae\u30b7\u30ea\u30fc\u30ba\u306e\u88fd\u54c1\u306e\u9055\u3044\u3092\u6559\u3048\u3066\u304f\u3060\u3055\u3044\u3002"},"content":{"rendered":"\n

\u5404\u88fd\u54c1\u306e\u9055\u3044\u3092\u88681\u306b\u307e\u3068\u3081\u307e\u3057\u305f\u3002
GoTaq\u00ae<\/sup> DNA Polymerase\u306f\u3001\u53cd\u5fdc\u30d0\u30c3\u30d5\u30a1\u30fc (5x)\u3068GoTaq\u00ae<\/sup> DNA Polymease\u3001dNTP\u3092\u5225\u3005\u306e\u30c1\u30e5\u30fc\u30d6\u304b\u3089\u53cd\u5fdc\u6db2\u306b\u6dfb\u52a0\u3059\u308b\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u3002\u4e00\u65b9\u3001GoTaq\u00ae<\/sup> Green Master Mix\u306f\u3001\u3053\u308c\u3089\u304c\u3059\u3079\u3066\u542b\u307e\u308c\u305f\u30d7\u30ec\u30df\u30c3\u30af\u30b9\u3068\u306a\u308a\u307e\u3059\u3002GoTaq\u00ae<\/sup> PCR Core System I,II\u306f\u3001\u53cd\u5fdc\u306b\u5fc5\u8981\u306a\u5168\u3066\u306e\u8a66\u85ac\u3068\u512a\u308c\u305f\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c6\u30a3\u30f3\u30b0\u3092\u542b\u3080\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u304c\u6dfb\u4ed8\u3055\u308c\u305f\u88fd\u54c1\u3067\u3059\u3002\u3055\u3089\u306b\u3001GoTaq\u00ae<\/sup> PCR Core System II\u306b\u306f\u3001\u53cd\u5fdc\u306e\u30dd\u30b8\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3068\u306a\u308b\u92f3\u578b\u3068\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u30bb\u30c3\u30c8\u3082\u542b\u307e\u308c\u307e\u3059\u3002<\/p>\n\n\n\n

\u88681<\/strong><\/p>\n\n\n\n

\u88fd\u54c1\u540d<\/th>\u30ab\u30bf\u30ed\u30b0
\u756a\u53f7<\/th>		\u69cb\u6210\u54c1<\/th>\u30d7\u30ec
\u30df\u30c3\u30af\u30b9
\u30bf\u30a4\u30d7<\/th>		\u8272\u7d20
\u542b\u6709<\/th>		dNTP
\u542b\u6709<\/th>		MgCl2<\/sub>
\u542b\u6709<\/th>		\u5099\u8003<\/th><\/tr>
GoTaq\u00ae<\/sup>
Green Master
Mix<\/td>		M7122
(100\u56de\u5206)a)<\/sup>
M7123
(1000\u56de\u5206)a)<\/sup><\/td>		\u30fb2XGoTaq\u00ae<\/sup> Green Master Mix
\u30fbNuclease Free Water<\/td>		\u25cb<\/td>\u25cb<\/td>\u25cb<\/td>\u25cb<\/td> <\/td><\/tr>
GoTaq\u00ae<\/sup>
Colorless
Master Mix<\/td>		M7132
(100\u56de\u5206)a)<\/sup>
M7133
(1000\u56de\u5206)a)<\/sup><\/td>		\u30fb2XGoTaq\u00ae<\/sup> colorless Master Mix
\u30fbNuclease Free Water<\/td>		\u25cb<\/td>\u00d7<\/td>\u25cb<\/td>\u25cb<\/td> <\/td><\/tr>
GoTaq\u00ae<\/sup> DNA Polymease<\/td>M3001(100u) M3002(500u)
M3003(2500u)<\/td>		\u30fbGoTaq\u00ae<\/sup> DNA Polymerase
\u30fb5X Green GoTaq\u00ae<\/sup> Reaction Buffer
\u30fb5X Green Colorless GoTaq\u00ae<\/sup> Reaction Buffer<\/td>		\u00d7<\/td>\u25cb
\u9078\u629e\u53efb)<\/sup><\/td>		\u5225\u9014\u8cfc\u5165c)
<\/sup>\uff08\u8abf\u88fd\u6642\u306b\u53cd\u5fdc
\u6db2\u306b\u6dfb\u52a0\uff09<\/td>		\u25cb<\/td> <\/td><\/tr>
GoTaq\u00ae<\/sup>
Flexi DNA
Polymease<\/td>		M8291(100u) M8295(500u)
M8296(5\u00d7500u)<\/td>		\u30fbGoTaq\u00ae<\/sup> Flexi DNA Polymerase
\u30fb5X Green GoTaq\u00ae<\/sup> Flexi Reaction Buffer
\u30fb5X Green Colorless GoTaq\u00ae<\/sup> Flexi Reaction Buffer
\u30fbMagnesium Chloride Solution, 25mM<\/td>		\u00d7<\/td>\u25cb
\u9078\u629e\u53efb)<\/sup><\/td>		\u5225\u9014\u8cfc\u5165c)
<\/sup>\uff08\u8abf\u88fd\u6642\u306b\u53cd\u5fdc
\u6db2\u306b\u6dfb\u52a0\uff09<\/td>		\u5225\u5305\u88c5
\uff08\u8abf\u88fd\u6642\u306b\u53cd\u5fdc
\u6db2\u306b\u6dfb\u52a0\uff09<\/td>		 <\/td><\/tr>
GoTaq\u00ae<\/sup>
PCR Core System I<\/td>		M7660\uff08200\u56de\u5206\uff09<\/td>\u30fbGoTaq\u00ae<\/sup> DNA Polymerase
\u30fb5X Colorless GoTaq\u00ae<\/sup> Flexi Buffer, Mg-Free
\u30fb5X Green GoTaq\u00ae<\/sup> Flexi Buffer, Mg-Free
\u30fbMgCl2<\/sub>
\u30fbPCR Nucleotide Mix, 10mM<\/td>		\u00d7<\/td>\u25cb
\u9078\u629e\u53efb)<\/sup><\/td>		\u5225\u5305\u88c5
\uff08\u8abf\u88fd\u6642\u306b\u53cd\u5fdc \u6db2\u306b\u6dfb\u52a0\uff09<\/td>		\u5225\u5305\u88c5
\uff08\u8abf\u88fd\u6642\u306b\u53cd\u5fdc
\u6db2\u306b\u6dfb\u52a0\uff09<\/td>		 <\/td><\/tr>
GoTaq\u00ae<\/sup>
PCR Core System II<\/td>		M7665\uff08200\u56de\u5206\uff09<\/td>\u30fbGoTaq\u00ae<\/sup> DNA Polymerase
\u30fb5X Colorless GoTaq\u00ae<\/sup> Flexi Buffer, Mg-Free
\u30fb5X Green GoTaq\u00ae<\/sup> Flexi Buffer, Mg-Free
\u30fbMgCl2<\/sub>
\u30fbPCR Nucleotide Mix, 10mM
\u30fbPositive Control Plasmid DNA, 1ng\/\u00b5l in TE buffer
\u30fbUpstream Control Primer, 15\u00b5M
\u30fbDownstream Control Primer, 15\u00b5M<\/td>		\u00d7<\/td>\u25cb
\u9078\u629e\u53efb)<\/sup><\/td>		\u5225\u5305\u88c5
\uff08\u8abf\u88fd\u6642\u306b\u53cd\u5fdc \u6db2\u306b\u6dfb\u52a0\uff09<\/td>		\u5225\u5305\u88c5
\uff08\u8abf\u88fd\u6642\u306b\u53cd\u5fdc
\u6db2\u306b\u6dfb\u52a0\uff09<\/td>		PCR\u306e\u30dd\u30b8\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3068\u306a\u308b\u92f3\u578b\u3068\u30d7\u30e9\u30a4\u30de\u30fc\u30bb\u30c3\u30c8\u304c\u542b\u307e\u308c\u307e\u3059\u3002<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

a)<\/sup>\uff1a\u53cd\u5fdc\u56de\u6570\u306f\u3001PCR\u53cd\u5fdc\u6eb6\u6db250uL\u30921\u53cd\u5fdc\u3068\u3057\u3066\u3044\u307e\u3059\u3002
b)<\/sup>\uff1a\u8272\u7d20\u3092\u542b\u3080Green\u3000Buffer\u3068\u8272\u7d20\u3092\u542b\u307e\u306a\u3044Colorless\u3000Buffer\u306e\u3069\u3061\u3089\u304b\u3092\u8abf\u88fd\u6642\u306b\u9078\u629e\u53ef\u80fd\u3067\u3059\u3002
c)<\/sup>\uff1aPCR Nucleotide Mix(C1141)<\/a>\u3084dNTPs(U1330)<\/a>\u306a\u3069\u304c\u3054\u5229\u7528\u3044\u305f\u3060\u3051\u307e\u3059\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u5404\u88fd\u54c1\u306e\u9055\u3044\u3092\u88681\u306b\u307e\u3068\u3081\u307e\u3057\u305f\u3002GoTaq\u00ae DNA Polymerase\u306f\u3001\u53cd\u5fdc\u30d0\u30c3\u30d5\u30a1\u30fc (5x)\u3068GoTaq\u00ae DNA Polymease\u3001dNTP\u3092\u5225\u3005\u306e\u30c1\u30e5\u30fc\u30d6\u304b\u3089\u53cd\u5fdc\u6db2\u306b\u6dfb\u52a0\u3059\u308b\u3053\u3068\u306b\u306a … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[86],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/159"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=159"}],"version-history":[{"count":3,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/159\/revisions"}],"predecessor-version":[{"id":2422,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/159\/revisions\/2422"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=159"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=159"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=159"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}