{"id":1591,"date":"2020-12-03T16:25:23","date_gmt":"2020-12-03T07:25:23","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1591"},"modified":"2020-12-22T16:39:46","modified_gmt":"2020-12-22T07:39:46","slug":"q295","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/proteomics\/invitro-transcription-translation\/kits\/tnt-system\/q295","title":{"rendered":"\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306f\u3069\u308c\u3092\u9078\u629e\u3059\u308c\u3070\u3088\u3044\u3067\u3059\u304b\uff1f"},"content":{"rendered":"\n

TNT\u00ae<\/sup> System\u3067\u306f\u3001SP6\u3001T7\u3001T3\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306e\u3044\u305a\u308c\u304b\u3092\u9078\u629e\u3057\u307e\u3059\u3002\u3053\u306e3\u3064\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306f\u3001\u30d0\u30af\u30c6\u30ea\u30aa\u30d5\u30a1\u30fc\u30b8\u7531\u6765\u306eRNA Polymerase\u306b\u3088\u308a\u92f3\u578bDNA\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u914d\u5217\u3092\u7279\u7570\u7684\u306b\u8a8d\u8b58\u3057\u3066RNA\u3092\u5408\u6210\u3057\u307e\u3059\u3002\u305d\u308c\u305e\u308c\u306eRNA polymerase\u306e\u6027\u8cea\u3084\u7279\u5fb4\u306f\u3001\u4f3c\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059(1-4)\u3002<\/p>\n\n\n\n

\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306f\u3001TNT\u00ae<\/sup> System\u30e9\u30a4\u30bb\u30fc\u30c8\u3001\u92f3\u578bDNA\u306e\u7d44\u307f\u5408\u308f\u305b\u3067\u9078\u629e\u3059\u308b\u3053\u3068\u3082\u3067\u304d\u307e\u3059\uff08\u88684\uff09\u3002<\/p>\n\n\n\n

\u88684\u3000 TNT\u00ae<\/sup> System\u306e\u30e9\u30a4\u30bb\u30fc\u30c8\u3068\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306e\u7d44\u307f\u5408\u308f\u305b<\/p>\n\n\n\n

TNT\u00ae<\/sup> System<\/th>SP6 Promoter<\/th>T7 Promoter<\/th>T3 Promoter<\/th><\/tr>
TNT\u00ae<\/sup> RRL<\/td>\u74b0\u72b6DNA\u3092\u4f7f\u7528\u3057\u305f\u5834\u5408\u306b\u6700\u3082\u52b9\u7387\u306e\u3088\u3044\u7ffb\u8a33\u304c\u3067\u304d\u307e\u3059\u3002
\u76f4\u9396DNA\u306e\u4f7f\u7528\u306f\u63a8\u5968\u3057\u307e\u305b\u3093<\/span><\/em>\u3002<\/td>		\u74b0\u72b6DNA\u3092\u4f7f\u7528\u3057\u305f\u5834\u5408\u306b\u6700\u3082\u52b9\u7387\u306e\u3088\u3044\u7ffb\u8a33\u304c\u3067\u304d\u307e\u3059\u3002
\u76f4\u9396DNA\u306e\u4f7f\u7528\u306f\u3001\u8a31\u5bb9\u7bc4\u56f2\u5185<\/span><\/em>\u3067\u3059\u3002<\/td>		\u74b0\u72b6DNA\u3092\u4f7f\u7528\u3057\u305f\u5834\u5408\u306b\u6700\u3082\u52b9\u7387\u306e\u3088\u3044\u7ffb\u8a33\u304c\u3067\u304d\u307e\u3059\u3002
\u76f4\u9396DNA\u306e\u4f7f\u7528\u306f\u3001\u8a31\u5bb9\u7bc4\u56f2\u5185<\/span><\/em>\u3067\u3059\u3002<\/td><\/tr>
TNT\u00ae<\/sup> WGE<\/td>\u74b0\u72b6DNA\u3092\u92f3\u578b\u3068\u3057\u3066\u7528\u3044\u305f\u5834\u5408\u306f\u3001\u6700\u3082\u52b9\u7387\u306e\u3088\u3044\u7ffb\u8a33\u304c\u3067\u304d\u307e\u3059\u3002
\u76f4\u9396DNA\u306e\u4f7f\u7528\u306f\u63a8\u5968\u3057\u307e\u305b\u3093<\/span><\/em>\u3002<\/td>		\u74b0\u72b6DNA\u306e\u4f7f\u7528\u306f\u63a8\u5968\u3057\u307e\u305b\u3093<\/span><\/em>\u3002
\u76f4\u9396DNA\u3092\u4f7f\u7528\u3057\u305f\u5834\u5408\u306f\u3001\u30b3\u30fc\u30c7\u30a3\u30f3\u30b0\u9818\u57df\u306e\u4e0b\u6d41\u306bT7 termination\u914d\u5217\u304c\u5fc5\u8981\u3067\u3059<\/span><\/em>\u3002<\/td>		\u74b0\u72b6DNA\u3001\u76f4\u9396DNA\u3044\u305a\u308c\u3082\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

\u53c2\u8003\u6587\u732e\uff1a<\/p>\n\n\n\n

  1. Butler, E.T. and Chamberlin, M.J. (1982) J. Biol. Chem. 257, 5772-5778.<\/li>
  2. Chamberlin, M., McGrath, J. and Waskell, L. (1970) Nature 228, 227-231.<\/li>
  3. Dunn, J.J., Bautz, F.A. and Bautz, E.K.F. (1971) Nature (London) New Biol. 230, 94-96.<\/li>
  4. Chamberlin, M. and Ryan, T. (1982) In The Enzymes (Boyer, P.D., ed.), Vol. 15, Nucleic Acids, Part B, pp. 87-108, Academic Press, New York.<\/li><\/ol>\n","protected":false},"excerpt":{"rendered":"

    TNT\u00ae System\u3067\u306f\u3001SP6\u3001T7\u3001T3\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306e\u3044\u305a\u308c\u304b\u3092\u9078\u629e\u3057\u307e\u3059\u3002\u3053\u306e3\u3064\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306f\u3001\u30d0\u30af\u30c6\u30ea\u30aa\u30d5\u30a1\u30fc\u30b8\u7531\u6765\u306eRNA Polymerase\u306b\u3088\u308a\u92f3\u578bDNA\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u914d\u5217\u3092\u7279\u7570\u7684\u306b\u8a8d\u8b58\u3057 … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[151],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1591"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1591"}],"version-history":[{"count":4,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1591\/revisions"}],"predecessor-version":[{"id":1963,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1591\/revisions\/1963"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1591"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1591"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1591"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}