{"id":1598,"date":"2020-12-03T16:26:17","date_gmt":"2020-12-03T07:26:17","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1598"},"modified":"2020-12-18T14:18:44","modified_gmt":"2020-12-18T05:18:44","slug":"q299","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/proteomics\/invitro-transcription-translation\/kits\/tnt-system\/q299","title":{"rendered":"\u7ffb\u8a33\u52b9\u7387\u3092\u9ad8\u3081\u308b\u305f\u3081\u306e\u56e0\u5b50\u306f\u3042\u308a\u307e\u3059\u304b\uff1f\u307e\u305f\u3001pTNT\u00ae Vector\u3001 pCMV TNT\u00ae Vector\u306b\u3042\u308b\u03b2-\u30b0\u30ed\u30d3\u30f3\u30ea\u30fc\u30c0\u30fc\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u3068\u306f\u4f55\u3067\u3059\u304b\uff1f"},"content":{"rendered":"\n<p>\u03b2-\u30b0\u30ed\u30d3\u30f3\u30ea\u30fc\u30c0\u30fc\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u306f\u3001\u5916\u90e8\u304b\u3089\u633f\u5165\u3057\u305f\u7570\u7a2emRNA\u306e\u8ee2\u5199\u52b9\u7387\u3092\u4e0a\u3052\u308b\u305f\u3081\u306b\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u3002\u03b2-\u30b0\u30ed\u30d3\u30f3\u30ea\u30fc\u30c0\u30fc\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u30925\u2019UTR\u306b\u4ed8\u52a0\u3059\u308b\u3053\u3068\u3067\u3001\u3055\u307e\u3056\u307e\u306a\u7a2e\u985e\u306e\u907a\u4f1d\u5b50\u306e\u7ffb\u8a33\u52b9\u7387\u306e\u5897\u52a0\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u307e\u3059(13, 14)\u3002<\/p>\n\n\n\n<p>&lt;\u53c2\u8003&gt;<br>\u7ffb\u8a33\u52b9\u7387\u3092\u9ad8\u3081\u308b\u56e0\u5b50\u3068\u3057\u3066\u3001\u4ee5\u4e0b\u306e\u70b9\u304c\u6319\u3052\u3089\u308c\u307e\u3059\u3002<\/p>\n\n\n\n<ol><li>\u958b\u59cb\u30b3\u30c9\u30f3\u524d\u5f8c\u306eKozak\u306e\u30b3\u30f3\u30bb\u30f3\u30b5\u30b9\u914d\u5217<\/li><li>PolyA Tail\u307e\u305f\u306f3\u2019\u975e\u7ffb\u8a33\u9818\u57df(3\u2019UTR)<\/li><li>5\u2019 \u30ad\u30e3\u30c3\u30d7\u69cb\u9020\uff08m7G(5)ppp(5)G Cap Analog\uff09\u306f\u3001\u7ffb\u8a33\u958b\u59cb\u56e0\u5b50\u3068\u7d50\u5408\u3057\u307e\u3059\u3002<\/li><li>5\u2019\u975e\u7ffb\u8a33\u9818\u57df(5\u2019UTR)\u306bIRES\u306e\u914d\u5217\u3092\u4ed8\u52a0\u3059\u308b\u3053\u3068\u3067\u3001\u7ffb\u8a33\u52b9\u7387\u304c\u5411\u4e0a\u3059\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u3066\u3044\u307e\u3059\u3002<\/li><\/ol>\n\n\n\n<p>\u53c2\u8003\u6587\u732e\uff1a<\/p>\n\n\n\n<ol start=\"13\"><li>Falcone, D. and Andrews, D.W. (1991) Both the 5\u00b4 untranslated region and the sequences sur-rounding the start site contribute to efficient initiation of translation in vitro. Mol. Cell. Biol.11, 2656\u201364..<\/li><li>Annweiler, A., Hipskind, R.A. and Wirth, T. (1991) A strategy for efficient in vitro translation of cDNAs using the rabbit \u03b2 -globin leader sequence. Nucl. Acids Res. 19, 3750.<\/li><\/ol>\n","protected":false},"excerpt":{"rendered":"<p>\u03b2-\u30b0\u30ed\u30d3\u30f3\u30ea\u30fc\u30c0\u30fc\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u306f\u3001\u5916\u90e8\u304b\u3089\u633f\u5165\u3057\u305f\u7570\u7a2emRNA\u306e\u8ee2\u5199\u52b9\u7387\u3092\u4e0a\u3052\u308b\u305f\u3081\u306b\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u3002\u03b2-\u30b0\u30ed\u30d3\u30f3\u30ea\u30fc\u30c0\u30fc\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u30925\u2019UTR\u306b\u4ed8\u52a0\u3059\u308b\u3053\u3068\u3067\u3001\u3055\u307e\u3056\u307e\u306a\u7a2e\u985e\u306e\u907a\u4f1d\u5b50\u306e\u7ffb\u8a33\u52b9\u7387\u306e\u5897\u52a0\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u307e &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[151],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1598"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1598"}],"version-history":[{"count":1,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1598\/revisions"}],"predecessor-version":[{"id":1601,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1598\/revisions\/1601"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1598"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1598"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1598"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}