{"id":1614,"date":"2020-12-03T16:27:47","date_gmt":"2020-12-03T07:27:47","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1614"},"modified":"2020-12-18T16:21:48","modified_gmt":"2020-12-18T07:21:48","slug":"q306","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/proteomics\/invitro-transcription-translation\/kits\/tnt-system\/q306","title":{"rendered":"TNT\u00ae System\u3067\u7ffb\u8a33\u3055\u305b\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u3092\u78ba\u8a8d\u3057\u305f\u3068\u304d\u306b\u30b5\u30f3\u30d7\u30eb\u4ee5\u5916\u306e\u30a8\u30ad\u30b9\u30c8\u30e9\u30d0\u30f3\u30c9\u304c\u8a8d\u3081\u3089\u308c\u307e\u3057\u305f\u3002\u3069\u306e\u3088\u3046\u306b\u3059\u308c\u3070\u3088\u3044\u3067\u3057\u3087\u3046\u304b\uff1f"},"content":{"rendered":"\n

\u4e3b\u306b\u3001\u4ee5\u4e0b\u306e\u70b9\u304c\u6319\u3052\u3089\u308c\u307e\u3059\u3002<\/p>\n\n\n\n

  1. TNT\u00ae<\/sup> System\u3067\u4f7f\u7528\u3057\u3066\u3044\u308bRRL\u306f\u30d8\u30e2\u30b0\u30ed\u30d3\u30f3\u7531\u6765\u306e42kDa\u306e\u30d0\u30c3\u30af\u30b0\u30e9\u30f3\u30c9\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u5834\u5408\u306f\u3001\u30e9\u30a4\u30bb\u30fc\u30c8\u3092\u900f\u6790\u306a\u3069\u3067\u9664\u53bb\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>
  2. Aminoacyl tRNA\u306e25kDa\u3082\u30d0\u30c3\u30af\u30b0\u30e9\u30f3\u30c9\u3068\u3057\u3066\u51fa\u73fe\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u5834\u5408\u306f\u3001RNase A\u3092TNT\u53cd\u5fdc\u5f8c\u306e\u6eb6\u6db2\u306b0.2mg\/mL\u3068\u306a\u308b\u3088\u3046\u6dfb\u52a0\u3057\u300130\u2103\u30015\u5206\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u3092\u884c\u3063\u3066\u304f\u3060\u3055\u3044\u3002<\/li>
  3. SDS-PAGE\u306e\u969b\u306b\u9178\u5316\u3057\u3066\u3057\u307e\u3063\u3066\u3044\u308b\u03b2-\u30e1\u30eb\u30ab\u30d7\u30c8\u30a8\u30bf\u30ce\u30fc\u30eb\u3092\u7528\u3044\u305f\u5834\u5408\u306b\u3082\u975e\u7279\u7570\u7684\u306a\u30d0\u30f3\u30c9\u304c\u51fa\u73fe\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002SDS-PAGE\u306b\u4f7f\u7528\u3059\u308bLoading Buffer\u3092\u306a\u308b\u3079\u304f\u30012% SDS\u3068100mM\u306eDTT\u3092\u4f7f\u7528\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li><\/ol>\n\n\n\n

    \u30e9\u30a4\u30bb\u30fc\u30c8\u7531\u6765\u306e\u30d0\u30c3\u30af\u30b0\u30e9\u30f3\u30c9\u306b\u3064\u3044\u3066\u306f\u3001\u5404\u88fd\u54c1\u30d7\u30ed\u30c8\u30b3\u30eb\u306e\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c6\u30a3\u30f3\u30b0\u306e\u9805\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

    \u4e3b\u306b\u3001\u4ee5\u4e0b\u306e\u70b9\u304c\u6319\u3052\u3089\u308c\u307e\u3059\u3002 TNT\u00ae System\u3067\u4f7f\u7528\u3057\u3066\u3044\u308bRRL\u306f\u30d8\u30e2\u30b0\u30ed\u30d3\u30f3\u7531\u6765\u306e42kDa\u306e\u30d0\u30c3\u30af\u30b0\u30e9\u30f3\u30c9\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u5834\u5408\u306f\u3001\u30e9\u30a4\u30bb\u30fc\u30c8\u3092\u900f\u6790\u306a\u3069\u3067\u9664\u53bb\u3057\u3066\u304f\u3060\u3055\u3044\u3002 Aminoacyl tR … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[151],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1614"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1614"}],"version-history":[{"count":1,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1614\/revisions"}],"predecessor-version":[{"id":1615,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1614\/revisions\/1615"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1614"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1614"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1614"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}