{"id":1700,"date":"2020-12-21T09:35:11","date_gmt":"2020-12-21T00:35:11","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1700"},"modified":"2021-02-16T09:35:12","modified_gmt":"2021-02-16T00:35:12","slug":"q309","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/proteomics\/invitro-transcription-translation\/kits\/e-coli-s30\/q309","title":{"rendered":"\u30d7\u30ed\u30e1\u30ac\u306eE. coli S30 Extract System\u306f\u3069\u306e\u3088\u3046\u306a\u88fd\u54c1\u3067\u3059\u304b\uff1f\u307e\u305f\u3001\u8907\u6570\u306e\u88fd\u54c1\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u305d\u308c\u305e\u308c\u3069\u306e\u3088\u3046\u306a\u7279\u5fb4\u304c\u3042\u308a\u307e\u3059\u304b\uff1f"},"content":{"rendered":"\n

E. coli S30 Extract System\u306f\u3001\u5927\u8178\u83cc\u7121\u7d30\u80de\u7ffb\u8a33\u30b7\u30b9\u30c6\u30e0\u3067\u3059\u3002\u88fd\u54c1\u3068\u3057\u3066\u306f3\u7a2e\u985e\u3042\u308a\u3001\u305d\u308c\u305e\u308c\u306e\u7279\u5fb4\u306f\u88681\u306e\u3068\u304a\u308a\u3067\u3059\u3002<\/p>\n\n\n\n

E. coli S30 System for Circular DNA<\/strong>\u306f\u3001\u8ee2\u5199\u30fb\u7ffb\u8a33\u306b\u5fc5\u8981\u306a\u6210\u5206(S30 Extract)\u3068\u30a2\u30df\u30ce\u9178\u30df\u30c3\u30af\u30b9\u4ee5\u5916\u306erNTPs\u3001tRNA\u3001ATP\u518d\u751f\u7cfb\u30b7\u30b9\u30c6\u30e0\u3001IPTG\u305d\u3057\u3066\u9069\u5207\u306a\u5869\u6fc3\u5ea6\u3092\u542b\u3093\u3067\u3044\u308bS30 Premix\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002in vitro\u3067\u7ffb\u8a33\u3055\u305b\u308b\u305f\u3081\u306b\u306f\u3001\u92f3\u578b\u306bE. coli\u306e strong promoter\u3067\u3042\u308blac\u3001tac\u306a\u3069\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u914d\u5217\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n

E. coli S30 System for Linear Template<\/strong>\u306f\u3001E. coli S30 System for Circular DNA<\/strong>\u3067\u4f7f\u7528\u3059\u308bS30 Extract\u3092\u4fee\u98fe\u3059\u308b\u3053\u3068\u3067\u3001\u76f4\u9396DNA\u3092\u92f3\u578b\u306b\u3057\u305f\u3068\u304d\u3067\u3082\u8ee2\u5199\u30fb\u7ffb\u8a33\u53cd\u5fdc\u3092\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u307e\u305f\u3001RNA\u3092\u92f3\u578b\u306b\u3057\u305f\u7ffb\u8a33\u53cd\u5fdc\u306e\u307f\u306b\u3082\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n

E. coli T7 S30 System for Circular DNA<\/strong>\u306f\u3001E. coli S30 System for Circular DNA<\/strong>\u3067\u4f7f\u7528\u3059\u308bS30 Extract\u3092\u4fee\u98fe\u3059\u308b\u3053\u3068\u3067T7 promoter\u306e\u914d\u5217\u3092\u3082\u3064\u74b0\u72b6DNA\u3092\u92f3\u578b\u3068\u3057\u3066\u3082\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u76f4\u9396DNA\u3001RNA\u306f\u92f3\u578b\u3068\u3057\u3066\u4f7f\u7528\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n\n\n\n

\uff1c\u53c2\u8003\uff1e<\/p>\n\n\n\n

S30\u3068\u306f\u3001supernatant-30,000g(30,000xg\u3067\u9060\u5fc3\u5f8c\u306b\u5f97\u3089\u308c\u305f\u4e0a\u6e05\u6db2)\u306e\u7565\u3067\u3059\u3002<\/p>\n\n\n\n

\u88681\u3000E. coli S30 Extract System\u306e\u6bd4\u8f03<\/strong><\/p>\n\n\n\n

\u88fd\u54c1\u540d<\/th>\u92f3\u578b<\/th>\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc<\/th>\u53ce\u91cf*<\/th>\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb
\u30d9\u30af\u30bf\u30fc<\/th><\/tr>
\u74b0\u72b6
DNA<\/th>		\u76f4\u9396\u72b6
DNA<\/th>		RNA<\/th><\/tr>
E. coli S30 System for Circular DNA<\/td>\u25cb<\/td>\u00d7<\/td>\u00d7<\/td>strong E. coli<\/td>100\uff5e250ng<\/td>pBESTluc\u2122 Vector Circular (1ug\/uL)<\/td><\/tr>
E. coli S30 System for Linear Template<\/td>\u25cb<\/td>\u25cb<\/td>\u25cb<\/td>strong E. coli<\/td>100\uff5e250ng<\/td>pBESTluc\u2122 Vector Linear (0.5ug\/uL)<\/td><\/tr>
E. coli T7 S30 System for Circular DNA<\/td>\u25cb<\/td>\u00d7<\/td>\u00d7<\/td>T7 \u30d7\u30ed\u30e2\u30fc\u30bf\u30fc,<\/span><\/strong> strong E. coli<\/td>300ng<\/td>PinPoint\u2122 Control Vector DNA Circular (1ug\/uL)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

*\uff1a1\u56de\u306e\u53cd\u5fdc\u91cf\u309250uL\u3068\u3057\u3066\u7b97\u51fa\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

E. coli S30 Extract System\u306f\u3001\u5927\u8178\u83cc\u7121\u7d30\u80de\u7ffb\u8a33\u30b7\u30b9\u30c6\u30e0\u3067\u3059\u3002\u88fd\u54c1\u3068\u3057\u3066\u306f3\u7a2e\u985e\u3042\u308a\u3001\u305d\u308c\u305e\u308c\u306e\u7279\u5fb4\u306f\u88681\u306e\u3068\u304a\u308a\u3067\u3059\u3002 E. coli S30 System for Circular DNA\u306f\u3001 … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[152],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1700"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1700"}],"version-history":[{"count":10,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1700\/revisions"}],"predecessor-version":[{"id":1978,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1700\/revisions\/1978"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1700"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1700"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1700"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}