{"id":1704,"date":"2020-12-21T09:37:35","date_gmt":"2020-12-21T00:37:35","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1704"},"modified":"2021-02-16T09:35:52","modified_gmt":"2021-02-16T00:35:52","slug":"q311","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/proteomics\/invitro-transcription-translation\/kits\/e-coli-s30\/q311","title":{"rendered":"E. coli S30 Extract System\u3092\u7528\u3044\u305f\u767a\u73fe\u7cfb\u3067\u306f\u3001\u92f3\u578bDNA\u3092\u3069\u306e\u3088\u3046\u306b\u8a2d\u8a08\u3059\u308c\u3070\u3088\u3044\u3067\u3059\u304b\uff1f"},"content":{"rendered":"\n

\u5927\u8178\u83cc\u3067\u767a\u73fe\u3055\u305b\u308b\u305f\u3081\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3068\u305d\u306e\u4e0b\u6d41\u306b\u9069\u5207\u306aRibosomal Binding Site (RBS)\u3092\u542b\u3080\u3088\u3046\u306b\u8a2d\u8a08\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u5927\u8178\u83cc\u3067\u3088\u304f\u767a\u73fe\u3059\u308b\u5171\u901a\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3068\u3057\u3066\u306f\u3001lacUV5\u3001tac\u3001\u03bbPL<\/sub>\u3001lac\u3001trp\u306a\u3069\u304c\u3042\u308a\u307e\u3059\u3002\u30d9\u30af\u30bf\u30fc\u3092\u7528\u3044\u3066\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u306e\u907a\u4f1d\u5b50\u3092\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3059\u308b\u5834\u5408\u306f\u3001\u30d9\u30af\u30bf\u30fc\u914d\u5217\u306b\u3042\u308b\u5927\u8178\u83cc\u767a\u73fe\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\u88682\u3000\u5927\u8178\u83cc\u767a\u73fe\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306e\u30b3\u30f3\u30bb\u30f3\u30b5\u30b9\u914d\u5217<\/strong><\/p>\n\n\n\n

\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc<\/th>-35 region<\/th>-10 region<\/th><\/tr>
\u30b3\u30f3\u30bb\u30f3\u30b5\u30b9\u914d\u5217 (1)<\/td>TTGACA<\/strong><\/td>TATAAT<\/strong><\/td><\/tr>
lacUV5 (2)<\/td>TTT<\/span>ACA<\/td>TAC<\/span>AATG<\/span><\/td><\/tr>
tac (2)<\/td>TTT<\/span>ACA<\/td>TT<\/span>TAATG<\/span><\/td><\/tr>
\u03bbPL<\/sub>(2)<\/td>TTGACA<\/td>ATAAT<\/td><\/tr>
lac (2)<\/td>TTT<\/span>ACA<\/td>TATGT<\/span>T<\/td><\/tr>
trp (2)<\/td>TTGACA<\/td>TTAACTA<\/span><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

\u958b\u59cb\u30b3\u30c9\u30f3\u304b\u3089\u9069\u5207\u306a\u9593\u9694\u3092\u3042\u3051\u3066\u4f4d\u7f6e\u3057\u3066\u3044\u308bRBS\u306f\u3001E. coli\u306e\u767a\u73fe\u7cfb\u3067\u7ffb\u8a33\u3092\u958b\u59cb\u3055\u305b\u308b\u305f\u3081\u306b\u5fc5\u8981\u3067\u3059\u3002\u4f8b\u3048\u3070\u3001pF1A(K) T7 Flexi\u00ae<\/sup> Vector(C8441\u3001C8451)\u306eRBS\u306f\u3001Shine-Dalgarno\uff08SD\uff09\u914d\u5217\u306e\u300cAGGAG\u300d(3)\u3092\u7528\u3044\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

E. coli T7 S30 System for Circular DNA<\/strong>\u3092\u4f7f\u7528\u3057\u3001T7 promoter\u306e\u914d\u5217\u3092\u4ecb\u3057\u305f\u8ee2\u5199\u53cd\u5fdc\u3092\u5b9f\u65bd\u3059\u308b\u5834\u5408\u306f\u3001\u4f8b\u3068\u3057\u3066\u3001\u4ee5\u4e0b\u306e\u3068\u304a\u308a\u306b\u92f3\u578bDNA\u306e\u914d\u5217\u8a2d\u8a08\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\uff1c\u53c2\u8003\uff1e<\/p>\n\n\n\n

\u5927\u8178\u83cc\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u3068\u3057\u3066\u8ca9\u58f2\u3055\u308c\u3066\u3044\u308b\u3001PinPointTM Xa-1 Vector\uff08V2031\uff09\u306etac\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u9818\u57df\u304b\u3089\u958b\u59cb\u30b3\u30c9\u30f3\u306e\u9593\u306e\u914d\u5217\u3092\u793a\u3057\u307e\u3059\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u53c2\u8003\u6587\u732e<\/p>\n\n\n\n

(1)Nucleic Acids Res., 17, 2933?2945, 1989
(2)JBC., 260, 3539-3541,1985
(3) Nature 254, 34, 1975<\/p>\n","protected":false},"excerpt":{"rendered":"

\u5927\u8178\u83cc\u3067\u767a\u73fe\u3055\u305b\u308b\u305f\u3081\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3068\u305d\u306e\u4e0b\u6d41\u306b\u9069\u5207\u306aRibosomal Binding Site (RBS)\u3092\u542b\u3080\u3088\u3046\u306b\u8a2d\u8a08\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u5927\u8178\u83cc\u3067\u3088\u304f\u767a\u73fe\u3059\u308b\u5171\u901a\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3068\u3057\u3066\u306f\u3001lacUV5\u3001tac\u3001\u03bbPL\u3001l … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[152],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1704"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1704"}],"version-history":[{"count":4,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1704\/revisions"}],"predecessor-version":[{"id":1985,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1704\/revisions\/1985"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1704"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1704"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1704"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}