{"id":1710,"date":"2020-12-21T09:40:50","date_gmt":"2020-12-21T00:40:50","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=1710"},"modified":"2021-02-16T09:36:47","modified_gmt":"2021-02-16T00:36:47","slug":"q314","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/proteomics\/invitro-transcription-translation\/kits\/e-coli-s30\/q314","title":{"rendered":"E. coli S30 Extract System\u3092\u7528\u3044\u305f\u767a\u73fe\u7cfb\u3067\u306f\u3001\u7ffb\u8a33\u53cd\u5fdc\u306b\u5f71\u97ff\u3092\u53ca\u307c\u3059\u30d1\u30e9\u30e1\u30fc\u30bf\u30fc\u3068\u3057\u3066\u4f55\u304c\u6319\u3052\u3089\u308c\u307e\u3059\u304b\uff1f"},"content":{"rendered":"\n

E. coli S30 Extract System\u3067\u306e\u7ffb\u8a33\u53cd\u5fdc\u306b\u5f71\u97ff\u3092\u53ca\u307c\u3059\u30d1\u30e9\u30e1\u30fc\u30bf\u30fc\u3092\u88683\u306b\u307e\u3068\u3081\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

\u88683\u3000E. coli S30 System\u306e\u7ffb\u8a33\u53cd\u5fdc\u306b\u5f71\u97ff\u3092\u53d7\u3051\u308b\u30d1\u30e9\u30e1\u30fc\u30bf\u30fc<\/strong><\/p>\n\n\n\n

\u30d1\u30e9\u30e1\u30fc\u30bf\u30fc<\/th>\u6fc3\u5ea6\u7bc4\u56f2<\/th>\u5099\u8003<\/th><\/tr>
Mg2+<\/sup><\/td>0-2 mM<\/td>S30\u53cd\u5fdc\u7cfb\u3067\u306f\u3001\u6700\u91cd\u8981\u30d1\u30e9\u30e1\u30fc\u30bf\u30fc\u3067\u3059\u3002<\/td><\/tr>
K+<\/sup><\/td>0-100 mM<\/td> <\/td><\/tr>
NH4+<\/sup><\/td>0-10 mM<\/td>\u6fc3\u5ea6\u304c50mM\u4ee5\u4e0a\u306b\u306a\u3063\u305f\u5834\u5408\u306f\u53cd\u5fdc\u963b\u5bb3\u304c\u751f\u3058\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002<\/td><\/tr>
glycerol<\/td>2%\u4ee5\u4e0b<\/td>2%\u4ee5\u4e0a\u3067\u306f\u3001\u7ffb\u8a33\u6d3b\u6027\u306f\u4f4e\u4e0b\u3057\u307e\u3059\u3002<\/td><\/tr>
Pharge Polymerase\uff08SP6 polymerase\u306a\u3069\uff09<\/td>\u6ef4\u5b9a\u306b\u3088\u308a\u6700\u9069\u306a\u6fc3\u5ea6\u3092\u6c7a\u5b9a\u3059\u308b<\/td>1\u53cd\u5fdc\u3042\u305f\u308a\u30011\u00b5L\u4ee5\u4e0a\u6dfb\u52a0\u3057\u306a\u3044\u3088\u3046\u306b\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306b\u542b\u307e\u308c\u308b\u30b0\u30ea\u30bb\u30ed\u30fc\u30eb\u304c\u7ffb\u8a33\u53cd\u5fdc\u3092\u963b\u5bb3\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

\uff1c\u53c2\u8003\uff1e<\/p>\n\n\n\n

E. coli S30 System\u3092\u7528\u3044\u305f\u7ffb\u8a33\u53cd\u5fdc\u3067\u76ee\u7684\u3068\u3059\u308b\u30b5\u30a4\u30ba\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u5f97\u3089\u308c\u306a\u304b\u3063\u305f\u5834\u5408\u306f\u3001\u4ee5\u4e0b\u306e\u70b9\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

  • SDS-PAGE\u306b\u3088\u308a\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u89e3\u7523\u7269\u304c\u78ba\u8a8d\u3055\u308c\u305f\u3068\u304d\u306f\u3001\u3069\u306e\u6642\u671f\u304b\u3089\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u89e3\u304c\u8d77\u304d\u3066\u3044\u308b\u304b\u3092\u8abf\u3079\u3001\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u306e\u6642\u9593\u3092\u691c\u8a0e\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/li>
  • [35S]Met\u306eRI\u6a19\u8b58\u3092\u7528\u3044\u3066\u3001\u30a2\u30df\u30ce\u9178\u914d\u5217\u306b\u30e1\u30c1\u30aa\u30cb\u30f3\u3092\u542b\u3080\u9ad8\u5206\u5b50\u91cf\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5408\u6210\u3059\u308b\u5834\u5408\u306f\u3001[35S]Met\u304c\u65e9\u3044\u5272\u5408\u3067\u6d88\u8cbb\u3055\u308c\u308b\u305f\u3081\u3001\u7ffb\u8a33\u53cd\u5fdc\u9014\u4e2d\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u7523\u7269\u3092\u5f97\u308b\u3053\u3068\u304c\u4e88\u60f3\u3055\u308c\u307e\u3059\u3002<\/li>
  • \u901a\u5e38\u306e\u53cd\u5fdc\u6761\u4ef6\u306f37\u2103\u30671\uff5e2\u6642\u9593\u3067\u3059\u304c\u300125\u2103\u30672\uff5e6\u6642\u9593\u53cd\u5fdc\u3055\u305b\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u7ffb\u8a33\u91cf\u3092\u5897\u52a0\u3055\u305b\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/li>
  • \u5185\u5728\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u3088\u308b\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30b3\u30fc\u30c7\u30a3\u30f3\u30b0\u9818\u57df\u5185\u3067\u306e\u5207\u65ad\u304c\u8a8d\u3081\u3089\u308c\u305f\u3068\u304d\u306f\u3001\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u958b\u88c2\u3057\u305f\u8907\u6570\u306e\u30d0\u30f3\u30c9\u3092\u7279\u5b9a\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u767a\u73fe\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li><\/ul>\n","protected":false},"excerpt":{"rendered":"

    E. coli S30 Extract System\u3067\u306e\u7ffb\u8a33\u53cd\u5fdc\u306b\u5f71\u97ff\u3092\u53ca\u307c\u3059\u30d1\u30e9\u30e1\u30fc\u30bf\u30fc\u3092\u88683\u306b\u307e\u3068\u3081\u307e\u3057\u305f\u3002 \u88683\u3000E. coli S30 System\u306e\u7ffb\u8a33\u53cd\u5fdc\u306b\u5f71\u97ff\u3092\u53d7\u3051\u308b\u30d1\u30e9\u30e1\u30fc\u30bf\u30fc \u30d1\u30e9\u30e1\u30fc\u30bf\u30fc \u6fc3\u5ea6\u7bc4\u56f2 \u5099 … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[152],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1710"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=1710"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1710\/revisions"}],"predecessor-version":[{"id":1812,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/1710\/revisions\/1812"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=1710"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=1710"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=1710"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}