{"id":195,"date":"2020-12-02T11:21:22","date_gmt":"2020-12-02T02:21:22","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=195"},"modified":"2021-02-16T10:48:09","modified_gmt":"2021-02-16T01:48:09","slug":"q371","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/transfection\/transfection-reagents\/multifectam\/q371","title":{"rendered":"DNA\u3068\u8a66\u85ac\u306f\u3001\u3069\u306e\u6bd4\u7387\u3067\u6df7\u5408\u3059\u308b\u5834\u5408\u304c\u6700\u3082\u5c0e\u5165\u52b9\u7387\u304c\u826f\u3044\u3067\u3059\u304b?"},"content":{"rendered":"\n

\u7d30\u80de\u306b\u3088\u3063\u3066\u6700\u9069\u306a\u6df7\u5408\u6bd4\u304c\u7570\u306a\u308a\u307e\u3059\u3002
\u3044\u304f\u3064\u304b\u306e\u6df7\u5408\u6bd4\u3092\u691c\u8a0e\u3057\u3066\u304a\u8a66\u3057\u3044\u305f\u3060\u304d\u6700\u9069\u306a\u6df7\u5408\u6bd4\u3092\u6c7a\u5b9a\u3057\u3066\u307f\u3066\u304f\u3060\u3055\u3044\u3002
\u30d7\u30e9\u30b9\u30df\u30c9\u3068MultiFectam\u306e\u6700\u9069\u306a\u6df7\u5408\u6bd4\u3092\u691c\u8a0e\u3059\u308b\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u3092\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002
24well<\/a>\u3067\u306e\u4f8b\u306896well<\/a>\u3067\u306e\u4f8b

1. \u69d8\u3005\u306a\u7d30\u80de\u57f9\u990a\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\u306b\u304a\u3051\u308b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u30b9\u30b1\u30fc\u30eb<\/p>\n\n\n\n

\n \n \n \n \n \n \n \n \n \n \n \n
 <\/td>\n Growth Area
\u203b<\/strong><\/td>\n 		\u57f9\u5730\u91cf
\uff08ml\uff09<\/strong><\/td>\n 		MultiFectam
\uff08 \u03bcl\uff09<\/strong><\/td>\n 		DNA -Tris \u6eb6\u6db2<\/strong><\/td>\n Opti-MEMR
\uff08\u03bcl\uff09<\/strong><\/td>\n 		1 \u30a6\u30a7\u30eb\u3042\u305f\u308a\u306e\u6dfb\u52a0\u91cf
\uff08\u03bcl\uff09<\/strong><\/td>\n <\/tr>\n
DNA\/total volume<\/strong><\/td>\n <\/tr>\n
96-well<\/td>\n 0.32 cm2<\/sup><\/td>\n 0.1<\/td>\n 2.5<\/td>\n 0.1 \u03bcg \/ 5.0 \u03bcl<\/td>\n 2.5<\/td>\n 10<\/td>\n <\/tr>\n
48-well<\/td>\n 0.82 cm2<\/sup><\/td>\n 0.2<\/td>\n 5.0<\/td>\n 0.2 \u03bcg \/ 10.0 \u03bcl<\/td>\n 5.0<\/td>\n 20<\/td>\n <\/tr>\n
24-well<\/td>\n 1.88 cm2<\/sup><\/td>\n 0.5<\/td>\n 12.5<\/td>\n 0.5 \u03bcg \/ 25.0 \u03bcl<\/td>\n 12.5<\/td>\n 50<\/td>\n <\/tr>\n
12-well<\/td>\n 3.83 cm2<\/sup><\/td>\n 1.0<\/td>\n 25.0<\/td>\n 1.0 \u03bcg \/ 50.0 \u03bc<\/td>\n 25.0<\/td>\n 100<\/td>\n <\/tr>\n
6-well<\/td>\n 9.4 cm2<\/sup><\/td>\n 2.5<\/td>\n 62.5<\/td>\n 2.5 \u03bcg \/ 125 \u03bc<\/td>\n 62.5<\/td>\n 250<\/td>\n <\/tr>\n
35 mm<\/td>\n 8.0 cm2<\/sup><\/td>\n 2.0<\/td>\n 50.0<\/td>\n 2.0 \u03bcg \/ 100 \u03bcl<\/td>\n 62.5<\/td>\n 250<\/td>\n <\/tr>\n
60 mm<\/td>\n 21 cm2<\/sup><\/td>\n 5.0<\/td>\n 125<\/td>\n 5.0 \u03bcg \/ 250 \u03bcl<\/td>\n 125<\/td>\n 500<\/td>\n <\/tr>\n
100 mm<\/td>\n 55 cm2<\/sup><\/td>\n 10.0<\/td>\n 250<\/td>\n 10 \u03bcg \/ 500 \u03bcl<\/td>\n 250<\/td>\n 1000<\/td>\n <\/tr>\n <\/tbody><\/table><\/figure>\n\n\n\n

\u203bCorningR culture dishes \u306e\u30c7\u30fc\u30bf\u306b\u3088\u308a\u307e\u3059\u3002MultiFectam \u3068\u30d7\u30e9\u30b9\u30df\u30c9DNA \u6eb6\u6db2\u3068\u306e\u6700\u9069\u6df7\u5408\u6bd4\u7387\u30fb\u6dfb\u52a0\u91cf\u306f\u3001\u7d30\u80de\u7a2e\u304a\u3088\u3073\u5b9f\u9a13\u6761\u4ef6\u306b\u3088\u308a\u7570\u306a\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n

2. MultiFectam \u3068\u30d7\u30e9\u30b9\u30df\u30c9DNA-Tris \u6eb6\u6db2\u306e\u6df7\u5408\u6bd4\u7387\u3068\u6dfb\u52a0\u91cf<\/p>\n\n\n\n

\n \n \n \n \n
\u6df7\u5408\u6bd4<\/strong><\/td>\n MultiFectam<\/strong><\/td>\n DNA -Tris \u6eb6\u6db2<\/strong><\/td>\n Opti-MEMR<\/strong><\/td>\n 1 \u30a6\u30a7\u30eb\u3042\u305f\u308a\u306e\u6dfb\u52a0\u91cf<\/strong><\/td>\n<\/tr>\n
DNA\/total volume<\/strong><\/td>\n<\/tr>\n
A\u3000\u203b1<\/td>\n 8.4 \u03bcl<\/td>\n 0.5 \u03bcg \/ 29.1 \u03bcl<\/td>\n 12.5 \u03bcl<\/td>\n 20 \uff0d 50 \u03bcl<\/td>\n<\/tr>\n
B\u3000\u203b2<\/td>\n 12.5 \u03bcl<\/td>\n 0.5 \u03bcg \/ 25.0 \u03bcl<\/td>\n 12.5 \u03bcl<\/td>\n 50 \uff0d 100 \u03bcl \u203b4<\/td>\n<\/tr>\n
C\u3000\u203b3<\/td>\n 16.7 \u03bcl<\/td>\n 0.5 \u03bcg \/ 20.8 \u03bcl<\/td>\n 12.5 \u03bcl<\/td>\n 50 \uff0d 100 \u03bcl \u203b4<\/td>\n<\/tr>\n<\/tbody><\/table><\/figure>\n\n\n\n

A\u203b1\uff1a\u9ad8\u767a\u73fe\u304c\u671f\u5f85\u3067\u304d\u307e\u3059\u304c\u3001\u6bd2\u6027\u304c\u307f\u3089\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u6dfb\u52a0\u91cf\u3092\u5c11\u306a\u304f\u3059\u308b\u3053\u3068\u3092\u304a\u52e7\u3081\u3044\u305f\u3057\u307e\u3059\u3002
B\u203b2\uff1a\u591a\u304f\u306e\u7d30\u80de\u7a2e\u306b\u304a\u3044\u3066\u6700\u9069\u306a\u6761\u4ef6\u3067\u3059\uff08\u6a19\u6e96\u6cd5\uff09\u3002
C\u203b3\uff1a\u4f4e\u6bd2\u6027\u306e\u6761\u4ef6\u3067\u3059\u304c\u3001\u767a\u73fe\u304c\u3084\u3084\u4f4e\u3044\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u6dfb\u52a0\u91cf\u3092\u5897\u3084\u3059\u3053\u3068\u3092\u304a\u52e7\u3081\u3044\u305f\u3057\u307e\u3059\u3002
\u3000\u203b4 \uff1a\u6bd2\u6027\u304c\u307f\u3089\u308c\u306a\u3044\u5834\u5408\u3001\u6dfb\u52a0\u91cf\u3092\u5897\u3084\u3059\u3053\u3068\u3067\u3088\u308a\u591a\u304f\u306e\u767a\u73fe\u304c\u307f\u3089\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u7d30\u80de\u306b\u3088\u3063\u3066\u6700\u9069\u306a\u6df7\u5408\u6bd4\u304c\u7570\u306a\u308a\u307e\u3059\u3002\u3044\u304f\u3064\u304b\u306e\u6df7\u5408\u6bd4\u3092\u691c\u8a0e\u3057\u3066\u304a\u8a66\u3057\u3044\u305f\u3060\u304d\u6700\u9069\u306a\u6df7\u5408\u6bd4\u3092\u6c7a\u5b9a\u3057\u3066\u307f\u3066\u304f\u3060\u3055\u3044\u3002\u30d7\u30e9\u30b9\u30df\u30c9\u3068MultiFectam\u306e\u6700\u9069\u306a\u6df7\u5408\u6bd4\u3092\u691c\u8a0e\u3059\u308b\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u3092\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u300224well\u3067\u306e\u4f8b … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[167],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/195"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=195"}],"version-history":[{"count":3,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/195\/revisions"}],"predecessor-version":[{"id":2045,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/195\/revisions\/2045"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=195"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=195"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=195"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}