{"id":2115,"date":"2020-12-28T12:21:58","date_gmt":"2020-12-28T03:21:58","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=2115"},"modified":"2021-01-27T14:01:10","modified_gmt":"2021-01-27T05:01:10","slug":"q407","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/genetic-reporter\/reporter-vector-and-cellline\/pnl-vector\/q407","title":{"rendered":"\u3044\u308d\u3044\u308d\u306a\u30d9\u30af\u30bf\u30fc\u304c\u3042\u308b\u3088\u3046\u3067\u3059\u304c\u3001\u3069\u306e\u3088\u3046\u306b\u4f7f\u3044\u5206\u3051\u305f\u3089\u3044\u3044\u3067\u3059\u304b\uff1f"},"content":{"rendered":"\n

\u5404pNL \u30d9\u30af\u30bf\u30fc\u306e\u7528\u9014\uff1a
pNL 1.x.\uff1a \u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u9818\u57df\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0
pNL 2.x.\uff1a \u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u9818\u57df\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0+ \u5b89\u5b9a\u7d30\u80de\u682a\u306e\u69cb\u7bc9\uff08\u30cf\u30a4\u30b0\u30ed\u30de\u30a4\u30b7\u30f3\u8010\u6027\u907a\u4f1d\u5b50\u3092\u542b\u3080\u30d9\u30af\u30bf\u30fc\uff09
pNL 3.x.\uff1a \u5fdc\u7b54\u30a8\u30ec\u30e1\u30f3\u30c8\u3084\u7d50\u5408\u30b5\u30a4\u30c8\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\uff08\u6700\u5c0f\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\uff08minP\uff09\u3092\u542b\u3080\u30d9\u30af\u30bf\u30fc\uff09
pNL1.1.CMV, pNL1.3.CMV\uff1a\u6a19\u6e96\u578b\u304a\u3088\u3073\u5206\u6ccc\u578bNluc\u7528\u306e\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30d7\u30e9\u30b9\u30df\u30c9
\u4ee5\u4e0b\u306epNL \u30d9\u30af\u30bf\u30fc\u4e00\u89a7\u8868\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002
\u5404\u7a2eNanoLuc\u2122 \u907a\u4f1d\u5b50\u306e\u4f7f\u3044\u5206\u3051\u306b\u3064\u304d\u307e\u3057\u3066\u306f\u300c\u30fbpNL Vector\u306e\u7279\u5fb4\u3092\u6559\u3048\u3066\u304f\u3060\u3055\u3044\u300d\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

Plasmid<\/th>Reporter<\/th>Marker<\/th>Promoter<\/th>MCS<\/th>Catalog#<\/th><\/tr>
pNL1.1<\/td>Nluc<\/td>–<\/td>–<\/td>Y<\/td>N1001<\/td><\/tr>
pNL1.2<\/td>NlucP<\/td>–<\/td>–<\/td>Y<\/td>N1011<\/td><\/tr>
pNL1.3<\/td>secNluc<\/td>–<\/td>–<\/td>Y<\/td>N1021<\/td><\/tr>
pNL2.1<\/td>Nluc<\/td>Hygro<\/td>–<\/td>Y<\/td>N1061<\/td><\/tr>
pNL2.2<\/td>NlucP<\/td>Hygro<\/td>–<\/td>Y<\/td>N1071<\/td><\/tr>
pNL2.3<\/td>secNluc<\/td>Hygro<\/td>–<\/td>Y<\/td>N1081<\/td><\/tr>
pNL3.1<\/td>Nluc<\/td>–<\/td>minP<\/td>Y<\/td>N1031<\/td><\/tr>
pNL3.2<\/td>NlucP<\/td>–<\/td>minP<\/td>Y<\/td>N1041<\/td><\/tr>
pNL3.3<\/td>secNluc<\/td>–<\/td>minP<\/td>Y<\/td>N1051<\/td><\/tr>
pNL1.1.CMV<\/td>Nluc<\/td>–<\/td>CMV<\/td>N<\/td>N1091<\/td><\/tr>
pNL1.3.CMV<\/td>secNluc<\/td>–<\/td>CMV<\/td>N<\/td>N1101<\/td><\/tr>
pNL3.2.NF-kB-RE<\/td>NlucP<\/td>Hygro<\/td>NF-kB-RE\/minP<\/td>N<\/td>N1111<\/td><\/tr><\/tbody><\/table><\/figure>\n","protected":false},"excerpt":{"rendered":"

\u5404pNL \u30d9\u30af\u30bf\u30fc\u306e\u7528\u9014\uff1apNL 1.x.\uff1a \u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u9818\u57df\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0pNL 2.x.\uff1a \u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u9818\u57df\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0+ \u5b89\u5b9a\u7d30\u80de\u682a\u306e\u69cb\u7bc9\uff08\u30cf\u30a4\u30b0\u30ed\u30de\u30a4\u30b7\u30f3\u8010\u6027\u907a\u4f1d\u5b50\u3092\u542b\u3080\u30d9\u30af\u30bf\u30fc\uff09pNL 3.x.\uff1a \u5fdc\u7b54\u30a8\u30ec\u30e1 … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[175],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/2115"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=2115"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/2115\/revisions"}],"predecessor-version":[{"id":2433,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/2115\/revisions\/2433"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=2115"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=2115"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=2115"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}