{"id":258,"date":"2020-12-02T12:11:29","date_gmt":"2020-12-02T03:11:29","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=258"},"modified":"2021-02-16T09:46:12","modified_gmt":"2021-02-16T00:46:12","slug":"q382","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/genetic-reporter\/reporter-assay\/reporter-info\/q382","title":{"rendered":"\u30d7\u30ed\u30e1\u30ac\u306e\u30ec\u30dd\u30fc\u30bf\u30fc\u9175\u7d20\u306e\u5b89\u5b9a\u6027\u3001\u5206\u5b50\u91cf\u3001\u57fa\u8cea\u3001\u304a\u3088\u3073\u611f\u5ea6\u306f\u3069\u306e\u3088\u3046\u306b\u306a\u3063\u3066\u3044\u307e\u3059\u304b\u3002"},"content":{"rendered":"\n<p>\u30d7\u30ed\u30e1\u30ac\u306e\u30db\u30bf\u30eb\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u3001\u30a6\u30df\u30b7\u30a4\u30bf\u30b1\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u3001\u03b2-\u30ac\u30e9\u30af\u30c8\u30b7\u30c0\u30fc\u30bc\u304a\u3088\u3073\u30af\u30ed\u30e9\u30e0\u30d5\u30a7\u30cb\u30b3\u30fc\u30eb\u30fb\u30a2\u30bb\u30c1\u30eb\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30e9\u30fc\u30bc\uff08CAT\uff09\u9175\u7d20\u306e\u5b89\u5b9a\u6027\u3001\u5206\u5b50\u91cf\u3001\u57fa\u8cea\u3001\u304a\u3088\u3073\u611f\u5ea6\u306e\u30c7\u30fc\u30bf\u3092\u4ee5\u4e0b\u306e\u8868\u306b\u793a\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u8868\u3000\u30ec\u30dd\u30fc\u30bf\u30fc\u9175\u7d20\u306e\u5b89\u5b9a\u6027\u30fb\u5206\u5b50\u91cf\u30fb\u57fa\u8cea\u30fb\u611f\u5ea6<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><th>\u30ec\u30dd\u30fc\u30bf\u30fc\u9175\u7d20<\/th><th>mRNA<br>\u534a\u6e1b\u671f<\/th><th>\u30bf\u30f3\u30d1\u30af\u8cea<br>\u534a\u6e1b\u671f<\/th><th>\u5206\u5b50\u91cf<\/th><th>\u57fa\u8cea<\/th><th>\u611f\u5ea6<\/th><\/tr><tr><td><strong>NanoLuc<br>\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc<\/strong><\/td><td><\/td><td><\/td><td>19kDa<br>\u5358\u91cf\u4f53<\/td><td>\u30d5\u30ea\u30de\u30b8\u30f3<\/td><td>10<sup>-22<\/sup>moles<br>(0.01 fg)<\/td><\/tr><tr><td><strong>\u30db\u30bf\u30eb<br>\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc<\/strong><\/td><td>6\u6642\u9593<br><sup>(1)<\/sup><\/td><td>3\u6642\u9593<br>(\u54fa\u4e73\u985e)<sup>(1,2)<\/sup><\/td><td>61kDa<br>\u5358\u91cf\u4f53<sup>(3)<\/sup><\/td><td>\u7532\u866b\u30eb\u30b7\u30d5\u30a7\u30ea\u30f3<br><sup>(1,6)<\/sup><\/td><td>10<sup>-20<\/sup>moles<br>(1 fg)<\/td><\/tr><tr><td><strong>\u30a6\u30df\u30b7\u30a4\u30bf\u30b1<br>\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc<\/strong><\/td><td>\uff0d<\/td><td>5.3\u6642\u9593<br>(40\u2103)<sup>(2)<\/sup><\/td><td>36kDa<br>\u5358\u91cf\u4f53<sup>(2,4,5)<\/sup><\/td><td>\u30bb\u30ec\u30f3\u30c6\u30e9\u30b8\u30f3<br><sup>(2,5,8)<\/sup><\/td><td>3X10<sup>-19<\/sup>moles<br>(10 fg)<\/td><\/tr><tr><td><strong>\u03b2-\u30ac\u30e9\u30af\u30c8\u30b7\u30c0\u30fc\u30bc<\/strong><\/td><td>\uff0d<\/td><td>20\u6642\u9593<\/td><td>465kDa 4\u91cf\u4f53<br>(\u5404116kDa)<sup>(6)<\/sup><\/td><td>ONPG<sup>(2,6)<\/sup><\/td><td>10<sup>4<\/sup>\uff5e10<sup>5<\/sup>moles<\/td><\/tr><tr><td><strong>CAT<\/strong><\/td><td>6\u6642\u9593<sup>(1)<\/sup><\/td><td>50\u6642\u9593<sup>(1)<\/sup><\/td><td>80kDa 4\u91cf\u4f53<br>(\u540420kDa)<sup>(7)<\/sup><\/td><td>\u30af\u30ed\u30e9\u30e0\u30d5\u30a7\u30cb\u30b3\u30fc\u30eb<br><sup>(6)<\/sup><\/td><td>5\uff5e10X10<sup>7<\/sup>moles<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>\u53c2\u8003\u8cc7\u6599\uff1a<\/p>\n\n\n\n<p>1. Thompson, J.F. et al. (1991) Gene 103, 171.<br>2. Bronstein, I. et al. (1994) Anal, Biochem. 219, 169<br>3. Luciferase Assay System Technical Bulletin #TB101, Promega Corporation.<br>4. Matthews, J.C. et al. (1977) Biochemistry 16, 85<br>5. Lorenz, W.W. et al. (1993) In : Bioluminescence and Chemiluminescence John Wiley and Sons, 191.<br>6. Alam, J. and Cook, J.L. (1990) Anal. Biochem. 188, 245<br>7. Shaw, V.W. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 4438.<br>8. Lorenz, W.W. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 4438<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30d7\u30ed\u30e1\u30ac\u306e\u30db\u30bf\u30eb\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u3001\u30a6\u30df\u30b7\u30a4\u30bf\u30b1\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u3001\u03b2-\u30ac\u30e9\u30af\u30c8\u30b7\u30c0\u30fc\u30bc\u304a\u3088\u3073\u30af\u30ed\u30e9\u30e0\u30d5\u30a7\u30cb\u30b3\u30fc\u30eb\u30fb\u30a2\u30bb\u30c1\u30eb\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30e9\u30fc\u30bc\uff08CAT\uff09\u9175\u7d20\u306e\u5b89\u5b9a\u6027\u3001\u5206\u5b50\u91cf\u3001\u57fa\u8cea\u3001\u304a\u3088\u3073\u611f\u5ea6\u306e\u30c7\u30fc\u30bf\u3092\u4ee5\u4e0b\u306e\u8868\u306b\u793a\u3057\u307e\u3059\u3002 \u8868\u3000\u30ec &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[172],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/258"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=258"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/258\/revisions"}],"predecessor-version":[{"id":2046,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/258\/revisions\/2046"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=258"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=258"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=258"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}