{"id":278,"date":"2020-12-02T12:19:49","date_gmt":"2020-12-02T03:19:49","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=278"},"modified":"2020-12-15T14:27:26","modified_gmt":"2020-12-15T05:27:26","slug":"q92","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/amplification-quantification\/pcr-and-rt\/rt\/m-mlv-reverse-transcriptase\/q92","title":{"rendered":"RNase H(-) \u306e\u9006\u8ee2\u5199\u9175\u7d20\u3092\u4f7f\u3046\u3053\u3068\u306f\u3001\u3069\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u3067\u3082\u3063\u3068\u3082\u6709\u52b9\u3067\u3059\u304b\u3002"},"content":{"rendered":"\n<p>RNase H(-)\u306e\u9006\u8ee2\u5199\u9175\u7d20\u306f\u3001cDNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u4f5c\u88fd\u306b\u304a\u3044\u3066\u9078\u629e\u3059\u3079\u304d\u9175\u7d20\u3067\u3059\u3002\u3053\u306e\u9175\u7d20\u306b\u3088\u308a\u9577\u3044\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u304b\u3089\u5b8c\u5168\u9577\u306ecDNA\u3092\u5408\u6210\u3067\u304d\u308b\u3068\u3044\u3046\u3053\u3068\u306f\u3001\u3088\u308a\u6b63\u78ba\u306aRNA\u306e\u76f8\u5bfe\u7684\u306a\u5b58\u5728\u91cf(\u8ee2\u5199\u7523\u7269\u306e\u9577\u30fb\u77ed\u306b\u3088\u3089\u305a\uff09\u3092\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306b\u53cd\u6620\u3067\u304d\u308b\u3068\u3044\u3046\u3053\u3068\u3067\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001RNase H(-)\u306e\u9006\u8ee2\u5199\u9175\u7d20\u3092\u4f7f\u7528\u3059\u308b\u4e00\u756a\u306e\u5229\u70b9\u304c\u9577\u9396cDNA\u3092\u5408\u6210\u3059\u308b\u3053\u3068\u3067\u3042\u308b\u305f\u3081\u3001\u901a\u5e38\u3067\u306f\u77ed\u3044DNA\u304c\u5fc5\u8981\u3068\u3055\u308c\u308bRT-PCR\u3084\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u30fb\u30a8\u30af\u30b9\u30c6\u30f3\u30b7\u30e7\u30f3\u306e\u7d50\u679c\u6539\u5584\u306b\u306f\u7d50\u3073\u3064\u304b\u306a\u3044\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u9577\u9396cDNA (&gt;5kb)\u304c\u5fc5\u8981\u3068\u3055\u308c\u308b\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u306f\u3001RNase H(-)\u306e\u9006\u8ee2\u5199\u9175\u7d20\u3092\u304a\u4f7f\u3044\u3044\u305f\u3060\u304f\u3053\u3068\u3092\u63a8\u5968\u3057\u307e\u3059\u3002\u9006\u8ee2\u5199\u9175\u7d20\u306e\u5b8c\u5168\u9577cDNA\u3092\u5408\u6210\u3059\u308b\u80fd\u529b\u306f, RNA\u306e\u9577\u3055\u306e\u307b\u304b\u306b\u3001\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u4e2d\u3067\u8907\u96d1\u306a\u4e8c\u6b21\u69cb\u9020\u3092\u3068\u308b\u9818\u57df\u3082\u5f71\u97ff\u3057\u307e\u3059\u3002\u53cd\u5fdc\u6e29\u5ea6\u3092\u4e0a\u3052\u3066cDNA\u5408\u6210\u53cd\u5fdc\u3092\u5b9f\u65bd\u3059\u308b\u3053\u3068\u3067\u3001\u554f\u984c\u3068\u306a\u308b\u4e8c\u6b21\u69cb\u9020\u3092\u6301\u3064\u9818\u57df\u3092\u30ea\u30e9\u30c3\u30af\u30b9\u3055\u305b\u3001\u5408\u6210\u53cd\u5fdc\u3092\u5411\u4e0a\u3055\u305b\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002RNA\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u4e2d\u306e\u4e8c\u6b21\u69cb\u9020\u304c\u5b8c\u5168\u9577cDNA\u306e\u5408\u6210\u3092\u963b\u3093\u3067\u3044\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u5834\u5408\u3084\u3001cDNA\u5408\u6210\u53cd\u5fdc\u3092\u6e29\u5ea6\u3092\u4e0a\u3052\u3066\u884c\u306a\u3044\u305f\u3044\u5834\u5408\u3001M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u3092\u304a\u85a6\u3081\u3057\u307e\u3059\u3002\u3044\u305a\u308c\u306e\u9175\u7d20\u3082\u9ad8\u6e29\u3067\u3054\u4f7f\u7528\u306b\u306a\u308c\u307e\u3059\u3002(PN75-Q&amp;A)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>RNase H(-)\u306e\u9006\u8ee2\u5199\u9175\u7d20\u306f\u3001cDNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u4f5c\u88fd\u306b\u304a\u3044\u3066\u9078\u629e\u3059\u3079\u304d\u9175\u7d20\u3067\u3059\u3002\u3053\u306e\u9175\u7d20\u306b\u3088\u308a\u9577\u3044\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u304b\u3089\u5b8c\u5168\u9577\u306ecDNA\u3092\u5408\u6210\u3067\u304d\u308b\u3068\u3044\u3046\u3053\u3068\u306f\u3001\u3088\u308a\u6b63\u78ba\u306aRNA\u306e\u76f8\u5bfe\u7684\u306a\u5b58\u5728\u91cf(\u8ee2\u5199\u7523\u7269\u306e\u9577\u30fb\u77ed\u306b\u3088\u3089 &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[92],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/278"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=278"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/278\/revisions"}],"predecessor-version":[{"id":1659,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/278\/revisions\/1659"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=278"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=278"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=278"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}