{"id":280,"date":"2020-12-02T12:20:21","date_gmt":"2020-12-02T03:20:21","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=280"},"modified":"2020-12-15T14:27:42","modified_gmt":"2020-12-15T05:27:42","slug":"q93","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/amplification-quantification\/pcr-and-rt\/rt\/m-mlv-reverse-transcriptase\/q93","title":{"rendered":"RNase H ( – )\u306e\u9006\u8ee2\u5199\u9175\u7d20\u3092\u4f7f\u3046\u3068\u3069\u3093\u306a\u5229\u70b9\u304c\u3042\u308a\u307e\u3059\u304b\uff1f"},"content":{"rendered":"\n

M-MLV Reverse Transcriptase, RNase H Minus\u306f\u5b8c\u5168\u9577cDNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u69cb\u7bc9\u306b\u6700\u9069\u306e\u9175\u7d20\u3067\u3059\u3002<\/p>\n\n\n\n

\u3000\u30d7\u30ed\u30e1\u30ac\u3067\u306fRNase H Minus\u9006\u8ee2\u5199\u9175\u7d20\u3068\u3057\u3066\u3001\u6b20\u5931\u5909\u7570\u578b(\u30ab\u30bf\u30ed\u30b0\u756a\u53f7M5301)\u3068\u70b9\u5909\u7570\u578b(\u30ab\u30bf\u30ed\u30b0\u756a\u53f7M3682)\u306e\u4e8c\u7a2e\u985e\u3092\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n

\u3000\u9006\u8ee2\u5199\u9175\u7d20[Reverse Transcriptase(RT)]\u306f\u3001RNA\u3092\u92f3\u578b\u306b\u3057\u3066\u76f8\u88dc\u7684\u306aDNA[complementary DNA(cDNA)]\u306e\u5408\u6210\u3092\u89e6\u5a92\u3059\u308bDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u4e00\u3064\u3067\u3059(1-3)\u3002RNA\u306b\u76f8\u88dc\u7684\u306acDNA\u3092\u5408\u6210\u3059\u308b\u9006\u8ee2\u5199\u9175\u7d20\u306e\u80fd\u529b\u306f\u3001cDNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u69cb\u7bc9\u3092\u306f\u3058\u3081\u3001RT-PCR(a)<\/sup>\u3084\u30d7\u30e9\u30a4\u30de\u30fc\u4f38\u9577\u6cd5\u3001RNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b7\u30f3\u30b0\u7b49\u3001\u5e45\u5e83\u3044\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u3067\u4f7f\u308f\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n

\u3000\u6700\u3082\u4e00\u822c\u7684\u306b\u4f7f\u308f\u308c\u3066\u3044\u308b\u9006\u8ee2\u5199\u9175\u7d20\u306b\u306f\u3001\u9ce5\u985e\u306e\u9aa8\u9ac4\u816b\u760d\u30a6\u30a4\u30eb\u30b9\u304b\u3089\u7cbe\u88fd\u3055\u308c\u308bAMV(Avian Myeloblastosis Virus)\u9006\u8ee2\u5199\u9175\u7d20(4,5)\u3068\u3001M-MLV(Moloney Murine Leukemia Virus)\u7531\u6765\u306e\u7d44\u66ff\u3048\u9175\u7d20\u3092\u751f\u7523\u3059\u308b\u3001\u5927\u8178\u83cc\u682a\u304b\u3089\u7cbe\u88fd\u3055\u308c\u308b\u9006\u8ee2\u5199\u9175\u7d20(6,7)\u306e2\u7a2e\u985e\u304c\u3042\u308a\u307e\u3059\u3002\u4e21\u9175\u7d20\u306f2\u3064\u306e\u4e3b\u8981\u306a\u6d3b\u6027\u3001RNA\u4f9d\u5b58\u6027DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u6d3b\u6027\u3068RNase H\u6d3b\u6027\u3092\u6709\u3057\u3066\u3044\u307e\u3059\u3002DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u6d3b\u6027\u306fcDNA\u5408\u6210\u304c\u5fc5\u8981\u306a\u5168\u3066\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u304a\u3044\u3066\u5fc5\u9808\u306e\u6d3b\u6027\u3067\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089RNase H\u6d3b\u6027\u306f\u3001\u5b8c\u5168\u9577\u306ecDNA\u306e\u751f\u6210\u306b\u5e72\u6e09\u3057\u3001\u671b\u307e\u3057\u3044\u6d3b\u6027\u3068\u306f\u8a00\u3048\u307e\u305b\u3093\u3002<\/p>\n\n\n\n

\u3000RNase H\u6d3b\u6027\u306f\u3001cDNA\u5408\u6210\u306b\u6700\u3082\u9069\u3057\u305f\u9006\u8ee2\u5199\u9175\u7d20\u3092\u9078\u629e\u3059\u308b\u969b\u306b\u3001\u91cd\u8981\u306a\u8003\u616e\u4e8b\u9805\u3068\u306a\u308a\u307e\u3059\u3002\u9006\u8ee2\u5199\u9175\u7d20\u306eRNase H\u6d3b\u6027\u306f\u5927\u8178\u83cc\u304c\u6709\u3059\u308bRNase H\u3068\u540c\u69d8\u306b\u3001RNA:DNA\u30cf\u30a4\u30d6\u30ea\u30c3\u30c9\u9396\u306eRNA\u9396\u3092\u5206\u89e3\u3057\u307e\u3059\u3002\u3044\u3063\u305f\u3093cDNA\u304c\u5408\u6210\u3055\u308c\u308b\u3068\u3001\u305d\u306eRNA:DNA\u30cf\u30a4\u30d6\u30ea\u30c3\u30c9\u9396\u306f\u9006\u8ee2\u5199\u9175\u7d20\u306eRNase H\u6d3b\u6027\u306e\u57fa\u8cea\u3068\u306a\u308a\u307e\u3059\u3002cDNA\u5408\u6210\u306b\u304a\u3051\u308bRNase H\u6d3b\u6027\u306f\u3001cDNA\u5168\u4f53\u306e\u5408\u6210\u91cf\u3068\u5b8c\u5168\u9577cDNA\u306e\u5272\u5408\u3092\u4f4e\u4e0b\u3055\u305b\u308b\u3068\u3044\u3063\u305f\u3001\u4e8c\u91cd\u306e\u5f71\u97ff\u3092\u4e0e\u3048\u307e\u3059\u3002\u9006\u8ee2\u5199\u306f\u92f3\u578bRNA\u306e\u7279\u7570\u7684\u306a\u914d\u5217(poly(A)\u30c6\u30fc\u30eb\u7b49)\u306b\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30ba\u3057\u305f\u3001DNA\u30d7\u30e9\u30a4\u30de\u30fc\u304b\u3089\u958b\u59cb\u3055\u308c\u307e\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc:RNA \u30cf\u30a4\u30d6\u30ea\u30c3\u30c9\u306f\u91cd\u5408\u53cd\u5fdc\u306e\u958b\u59cb\u70b9\u3068\u3057\u3066\u3060\u3051\u3067\u306a\u304f\u3001RNase H\u6d3b\u6027\u306e\u57fa\u8cea\u3068\u3057\u3066\u3082\u50cd\u304d\u307e\u3059\u3002\u5408\u6210\u3055\u308c\u305fcDNA\u306e\u53ce\u91cf\u306fRNA:DNA\u30cf\u30a4\u30d6\u30ea\u30c3\u30c9\u304cRNase H\u6d3b\u6027\u306b\u3088\u3063\u3066\u3069\u308c\u3060\u3051\u5206\u89e3\u3055\u308c\u308b\u304b\u3068\u3044\u3046\u3053\u3068\u306b\u53cd\u6bd4\u4f8b\u3057\u307e\u3059\u3002\u3044\u304f\u3064\u304b\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u3067\u306f\u3001cDNA\u5408\u6210\u3092\u691c\u51fa\u3059\u308b\u306e\u306b\u5fc5\u8981\u306aRNA\u306e\u6700\u5c0f\u91cf\u304c\u5272\u5897\u3057\u3066\u3057\u307e\u3044\u307e\u3059\u3002\u3055\u3089\u306b\u3001RNase H\u6d3b\u6027\u306fDNA\u306e\u91cd\u5408\u304c\u8d77\u3053\u3063\u3066\u3044\u308b\u4ed8\u8fd1\u306eRNA\u9396\u3092\u5206\u89e3\u3057\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u3001RNA\u306e\u30b3\u30d4\u30fc\u3055\u308c\u3066\u3044\u306a\u3044\u90e8\u5206\u304c\u8ee2\u5199\u8907\u5408\u4f53\u304b\u3089\u4e56\u96e2\u3057\u3001cDNA\u5408\u6210\u306f\u305d\u3053\u3067\u7d42\u7d50\u3057\u3066\u3057\u307e\u3044\u307e\u3059\u3002RNA\u5206\u5b50\u304c\u9577\u304f\u306a\u308c\u3070\u306a\u308b\u307b\u3069\u3001\u3053\u3046\u3057\u305f\u73fe\u8c61\u304c\u751f\u3058\u3084\u3059\u304f\u306a\u308a\u307e\u3059\u3002RNase H\u6d3b\u6027\u306e\u5b58\u5728\u4e0b\u306b\u304a\u3044\u3066\u3001\u9577\u3044RNA\u5206\u5b50(5kb\u4ee5\u4e0a)\u306f\u5b8c\u5168\u9577\u306ecDNA\u3092\u751f\u3058\u306b\u304f\u304f\u306a\u308a\u307e\u3059\u3002
\u3000\u9006\u8ee2\u5199\u9175\u7d20\u306eDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u6d3b\u6027\u306f\u4ed8\u968f\u3059\u308bRNase H\u6d3b\u6027\u306b\u4f9d\u5b58\u3057\u307e\u305b\u3093\u3002\u3053\u306e\u3088\u3046\u306bRNase H\u6d3b\u6027\u306fcDNA\u5408\u6210\u306b\u3068\u3063\u3066\u6709\u5bb3\u3067\u3059\u304c\u3001RNase H\u6d3b\u6027\u3092\u9664\u53bb\u3057\u305f\u9006\u8ee2\u5199\u9175\u7d20\u3092\u7528\u3044\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u554f\u984c\u3092\u89e3\u6d88\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u6d3b\u6027\u306e\u9664\u53bb\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u306eRNase H\u9818\u57df\u306b\u5909\u7570\u3092\u5c0e\u5165\u3059\u308b\u3053\u3068\u3067\u9054\u6210\u3055\u308c\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u9006\u8ee2\u5199\u9175\u7d20\u306fRNase H Minus\u3068\u547c\u3070\u308c\u307e\u3059\u3002RNase\u6d3b\u6027\u306e\u9664\u53bb\u306b\u3088\u308a\u3001primer:RNA\u30cf\u30a4\u30d6\u30ea\u30c3\u30c9\u304c\u5206\u89e3\u3055\u308c\u306b\u304f\u304f\u306a\u308b\u306e\u3067\u3001\u9006\u8ee2\u5199\u306e\u958b\u59cb\u53cd\u5fdc\u304c\u52b9\u7387\u826f\u304f\u884c\u308f\u308c\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3059\u3002\u3055\u3089\u306b\u3001\u91cd\u5408\u53cd\u5fdc\u304c\u8d77\u304d\u3066\u3044\u308b\u4ed8\u8fd1\u3067\u92f3\u578b\u304c\u5206\u89e3\u3055\u308c\u3001\u53cd\u5fdc\u304c\u7d42\u7d50\u3057\u3066\u3057\u307e\u3046\u50be\u5411\u3082\u306a\u304f\u306a\u308a\u3001\u5b8c\u5168\u9577cDNA\u306e\u5408\u6210\u304c\u3088\u308a\u52b9\u7387\u7684\u306b\u306a\u308a\u307e\u3059\u3002\u9ad8\u3044\u5272\u5408\u3067\u5b8c\u5168\u9577\u306ecDNA\u304c\u5fc5\u8981\u306a\uff43DNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u5408\u6210\u306a\u3069\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u3067\u306f\u3001RNase H Minus\u9006\u8ee2\u5199\u9175\u7d20\u3092\u63a8\u5968\u3057\u307e\u3059\u3002<\/p>\n\n\n\n

\u30002\u7a2e\u985e\u306eRNase H Minus\u9006\u8ee2\u5199\u9175\u7d20\u304c\u30d7\u30ed\u30e1\u30ac\u304b\u3089\u5165\u624b\u53ef\u80fd\u3067\u3059\u3002\u4e21\u9175\u7d20\u306fM-MLV\u9006\u8ee2\u5199\u9175\u7d20\u306e\u7d44\u307f\u63db\u3048\u4f53\u3067\u3059\u3002M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant(\u30ab\u30bf\u30ed\u30b0\u756a\u53f7M3682)\u306f\u3001RNase H\u9818\u57df\u306b\u70b9\u5909\u7570\u3092\u5c0e\u5165\u3059\u308b\u3053\u3068\u3067RNase H Minus\u306b\u3057\u307e\u3057\u305f\u3002\u672c\u88fd\u54c1\u306f\u3001RNase H minus reverse transcriptase, SuperScript? II\u3068\u3057\u3066\u826f\u304f\u77e5\u3089\u308c\u3066\u3044\u308b\u9175\u7d20\u3068\u6a5f\u80fd\u7684\u306b\u540c\u7b49\u306e\u88fd\u54c1\u3067\u3059\u3002\u3055\u3089\u306b\u3001\u30d7\u30ed\u30e1\u30ac\u3067\u306fM-MLV Reverse Transcriptase, RNase H Minus(\u30ab\u30bf\u30ed\u30b0\u756a\u53f7M5301)\u3068\u3044\u3046\u88fd\u54c1\u3082\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u672c\u88fd\u54c1\u306fRNase H\u9818\u57df\u3092\u6b20\u5931\u3055\u305b\u3066\u9175\u7d20\u6d3b\u6027\u3092\u9664\u3044\u3066\u3044\u3066\u3001\u6a5f\u80fd\u7684\u306bM-MLV Reverse Transcriptase, RNase H Minus, Point Mutant\u3068\u985e\u4f3c\u3059\u308b\u88fd\u54c1\u3067\u3059\u3002M-MLV Reverse Transcriptase, RNase H Minus\u306f\u4e00\u822c\u7684\u306b\u77e5\u3089\u308c\u308bRNase H minus reverse transcriptase, SuperScript? II\u3068\u6a5f\u80fd\u7684\u306b\u540c\u7b49\u3067\u3059\u3002<\/p>\n\n\n\n

\u53c2\u7167\u6587\u732e<\/strong><\/p>\n\n\n\n

  1. Eun, H.-M. (1996) Enzymology Primer for Recombinant DNA Technology, Academic Press, San Diego, California, 427.<\/li>
  2. Gerard, G.F. and D\u2019Alessio, J. (1993) In: Methods in Molecular Biology, Vol. 16: Enzymes of Molecular Biology, Burrell, M.M., ed., Humana Press, Totowa, New Jersey, 73.<\/li>
  3. Enzyme Resource Guide, Vol. 1: Polymerases BR075A, Promega Corporation.<\/li>
  4. Kacian, D.L. (1977) Meth. Virol. 6, 143.<\/li>
  5. Houts, G.E. et al. (1979) Reverse transcriptase from avian myeloblastosis virus. J. Virol. 29, 517.<\/li>
  6. Roth, M.J., Tanese, N. and Goff, S.P. (1985) Purification and characterization of murine retroviral reverse transcriptase expressed in Escherichia coli. J. Biol. Chem. 260, 9326.<\/li>
  7. Verma, I.M. (1974) In: The Enzymes, Boyer, P.D., ed., Academic Press, New York, 87.<\/li><\/ol>\n\n\n\n

    (a)The PCR process is covered by patents issued and applicable in certain countries. Promega does not encourage or support the unauthorized or unlicensed use of the PCR process.<\/p>\n\n\n\n

    SuperScript is a trademark of Life Technologies, Inc.<\/p>\n","protected":false},"excerpt":{"rendered":"

    M-MLV Reverse Transcriptase, RNase H Minus\u306f\u5b8c\u5168\u9577cDNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u69cb\u7bc9\u306b\u6700\u9069\u306e\u9175\u7d20\u3067\u3059\u3002 \u3000\u30d7\u30ed\u30e1\u30ac\u3067\u306fRNase H Minus\u9006\u8ee2\u5199\u9175\u7d20\u3068\u3057\u3066\u3001\u6b20\u5931\u5909\u7570\u578b(\u30ab\u30bf\u30ed\u30b0\u756a\u53f7 … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[92],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/280"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=280"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/280\/revisions"}],"predecessor-version":[{"id":283,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/280\/revisions\/283"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=280"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=280"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=280"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}