{"id":286,"date":"2020-12-02T12:27:31","date_gmt":"2020-12-02T03:27:31","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=286"},"modified":"2020-12-21T11:01:12","modified_gmt":"2020-12-21T02:01:12","slug":"q95","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/amplification-quantification\/pcr-and-rt\/rt-pcr\/rt-pcr-basic-info\/q95","title":{"rendered":"\u9006\u8ee2\u5199\u53cd\u5fdc\u306b\u306f\u3069\u3093\u306a\u9175\u7d20\u304c\u4f7f\u3048\u307e\u3059\u304b\u3002"},"content":{"rendered":"\n<p>\u4ee5\u4e0b\u9006\u8ee2\u5199\u9175\u7d20\u304c\u304a\u4f7f\u3044\u3044\u305f\u3060\u3051\u307e\u3059\u3002<br>\u30fbAMV (Avian Myeloblastosis Virus) (Cat.# M5101, M5108, M9004)<br>\u30fbM-MLV (Moloney Murine Leukemia Virus) (Cat.# M1701, M1705)<br>\u30fbM-MLV, RNase (H\u2013), Point Mutant (Cat.# M3681, M3682, M3683)<br>\u30fbM-MLV, RNase (H\u2013) (Cat.# M5301)<br>\u30fbGoScript Reverse Transcriptase (Cat.# A5003, A5004)<br><br><strong>\u3010\u9175\u7d20\u5206\u5b50\u3011<\/strong>\u00a0M-MLV RT\u306f71 kDa\u306e\u5358\u91cf\u4f53\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3059(1)\u3002AMV RT\u306f63 kDa\u306e\u03b1\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u306892 kDa \u306e\u03b2\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u304b\u3089\u306a\u308b155 kDa\u306e\u4e8c\u91cf\u4f53\u3067\u3059(2)\u3002GoScript\u306fM-MLV \u30d9\u30fc\u30b9\u3067\u3059\u3002<br><br><strong>\u3010RNase H\u6d3b\u6027\u3011<\/strong>\u00a0M-MLV RT\u306fAMV RT\u3088\u308a\u3082\u4f4e\u3044 RNase H \u6d3b\u6027\u3092\u793a\u3057\u307e\u3059(3)\u3002\u3053\u306e\u305f\u3081\u3001M-MLV RT\u306f\u3088\u308a\u9577\u3044 cDNA (>5kb) \u306e\u9006\u8ee2\u5199\u306b\u9069\u3057\u3066\u3044\u307e\u3059\u3002\u30d7\u30ed\u30e1\u30ac\u3067\u306fRMase H\u6d3b\u6027\u3092\u6d88\u5931\u3055\u305b\u305f\u5909\u7570\u4f53\u3082\u53d6\u308a\u6271\u3044\u3057\u3066\u304a\u308a\u307e\u3059\u3002<br><br><strong>\u3010\u53cd\u5fdc\u6e29\u5ea6\u3011<\/strong>\u00a0AMV RT \u306f M-MLV RT \u306b\u6bd4\u3079\u3066\u975e\u5e38\u306b\u9ad8\u3044 processivity (\u4e00\u5ea6\u7d50\u5408\u3057\u305f\u6642\u306b\u53d6\u308a\u8fbc\u307e\u308c\u308b\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u306e\u6570) \u3092\u793a\u3057\u3001\u3055\u3089\u306b\u3088\u308a\u9ad8\u3044\u53cd\u5fdc\u6e29\u5ea6\u3067\u6d3b\u6027\u3092\u793a\u3057\u307e\u3059\u3002\u4e00\u822c\u7684\u306b\u3001M-MLV RT \u306f 37\u2103 \u3067\u4f7f\u308f\u308c\u307e\u3059\u3002AMV RT \u306f 2 \u30b9\u30c6\u30c3\u30d7\u306eRT-PCR \u3067\u306f\u901a\u5e38 42\u2103 \u3067\u4f7f\u308f\u308c\u307e\u3059\u304c\u300158\u2103 \u307e\u3067\u6d3b\u6027\u304c\u7dad\u6301\u3055\u308c\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306b AMV RT \u306f\u9ad8\u6e29\u3067\u6d3b\u6027\u3092\u7dad\u6301\u3057\u3066\u3044\u308b\u305f\u3081\u3001\u5f37\u56fa\u306a\uff12\u6b21\u69cb\u9020\u3092\u6301\u3063\u305fRNA\u304b\u3089\u306e\u9006\u8ee2\u5199\u53cd\u5fdc\u306b\u9069\u3057\u3066\u3044\u307e\u3059\u3002GoScript Reverse Transcriptase\u306f\u6700\u65b0\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u6280\u8853\u3092\u63a1\u7528\u3057\u3066\u304a\u308a\u3001\u963b\u5bb3\u5264\u306e\u5b58\u5728\u4e0b\u3067\u3082\u5e0c\u5c11\u306a\u3082\u306e\u304b\u3089\u8c4a\u5bcc\u306a\u3082\u306e\u307e\u3067\u3001\u5e45\u5e83\u3044\u30ec\u30f3\u30b8\u306e\u8ee2\u5199\u7269\u3092cDNA\u306b\u5408\u6210\u3067\u304d\u308b\u3088\u3046\u306b\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u3066\u3044\u307e\u3059\u3002<br><br><strong>\u3010\u9006\u8ee2\u5199\u9175\u7d20\u3000\u6027\u80fd\u6bd4\u8f03\u8868\u3011<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"800\" height=\"256\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/pcr_rt_glaf_1.jpg\" alt=\"\" class=\"wp-image-1757\" srcset=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/pcr_rt_glaf_1.jpg 800w, https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/pcr_rt_glaf_1-768x246.jpg 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><figcaption>\uff08PN71-Q&amp;A)<\/figcaption><\/figure>\n\n\n\n<p><strong>\u53c2\u8003\u6587\u732e\uff1a<\/strong><\/p>\n\n\n\n<ol><li>(1) Roth, M.J., Tanese, N. and Goff, S.P. (1985) J. Biol. Chem. 260, 9326.<\/li><li>(2) Grandgenett, D.P., Gerard, G.F. and Green, M. (1973) Proc. Natl. Acad. Sci. USA 70, 230.<\/li><li>(3) Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) In: Molecular Cloning, A Laboratory Manual Vol. 1, Second Edition, 5.52.<\/li><\/ol>\n","protected":false},"excerpt":{"rendered":"<p>\u4ee5\u4e0b\u9006\u8ee2\u5199\u9175\u7d20\u304c\u304a\u4f7f\u3044\u3044\u305f\u3060\u3051\u307e\u3059\u3002\u30fbAMV (Avian Myeloblastosis Virus) (Cat.# M5101, M5108, M9004)\u30fbM-MLV (Moloney Murine Leukemia &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[94],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/286"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=286"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/286\/revisions"}],"predecessor-version":[{"id":1758,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/286\/revisions\/1758"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=286"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=286"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=286"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}