{"id":289,"date":"2020-12-02T12:29:23","date_gmt":"2020-12-02T03:29:23","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=289"},"modified":"2020-12-15T14:41:21","modified_gmt":"2020-12-15T05:41:21","slug":"q96","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/amplification-quantification\/pcr-and-rt\/rt-pcr\/rt-pcr-basic-info\/q96","title":{"rendered":"RT-PCR\u3067poly (A)+ RNA\u306e\u4ee3\u308f\u308a\u306bTotal RNA\u3092\u4f7f\u3048\u307e\u3059\u304b"},"content":{"rendered":"\n<p>cDNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u4f5c\u6210\u3092\u76ee\u7684\u3068\u3057\u3066\u3044\u306a\u3051\u308c\u3070\u3001RT-PCR\u306e\u92f3\u578b\u306bpoly(A)+ RNA\u306f\u5fc5\u8981\u3042\u308a\u307e\u305b\u3093\u30021\u03bcg\u306eTotal RNA\u3092\u30b5\u30f3\u30d7\u30eb\u3068\u3057\u3066\u5341\u5206\u306a\u7d50\u679c\u304c\u5f97\u3089\u308c\u307e\u3059\u3002poly(A)+ RNA\u3067\u306f\u3001\u5897\u5e45\u3055\u308c\u305fmRNA\u306e\u91cf\u306b\u3088\u308a\u3001\u901a\u5e381pg\u304b\u30891\u03bcg\u3067\u5341\u5206\u3067\u3059\u3002<\/p>\n\n\n\n<p>Total RNA\u306e\u30b5\u30f3\u30d7\u30eb\u3067\u306f\u3001\u9ad8\u6fc3\u5ea6\u306e\u30ea\u30dc\u30be\u30fc\u30de\u30ebRNA\u3068\u30c8\u30e9\u30f3\u30b9\u30d5\u30a1\u30fcRNA\u304c\u5b58\u5728\u3059\u308b\u305f\u3081\u3001\u30e9\u30f3\u30c0\u30e0\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u7528\u3044\u3066cDNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u4f5c\u6210\u3059\u308b\u3053\u3068\u306f\u4e00\u822c\u7684\u306b\u306f\u63a8\u5968\u3057\u307e\u305b\u3093\u3002\u3057\u304b\u3057\u3001PCR\u306f\u9ad8\u611f\u5ea6\u306a\u5897\u5e45\u304c\u53ef\u80fd\u306a\u305f\u3081\u3001\u30e9\u30f3\u30c0\u30e0\u30d7\u30e9\u30a4\u30de\u30fc\u30fboligo(dT) \u30d7\u30e9\u30a4\u30de\u30fc\u3001\u76ee\u7684\u306e\u907a\u4f1d\u5b50\u306b\u7279\u7570\u7684\u306a\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u7528\u3044\u3066\u3001\u7b2c1\u9396cDNA\u3092\u5408\u6210\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u30d7\u30ed\u30e1\u30ac\u3067\u306fReverse Transcription System(Cat.# A3500)\u306b\u30e9\u30f3\u30c0\u30e0\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u52a0\u3048\u307e\u3057\u305f\u3002\uff08PN71-Q&amp;A)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>cDNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u4f5c\u6210\u3092\u76ee\u7684\u3068\u3057\u3066\u3044\u306a\u3051\u308c\u3070\u3001RT-PCR\u306e\u92f3\u578b\u306bpoly(A)+ RNA\u306f\u5fc5\u8981\u3042\u308a\u307e\u305b\u3093\u30021\u03bcg\u306eTotal RNA\u3092\u30b5\u30f3\u30d7\u30eb\u3068\u3057\u3066\u5341\u5206\u306a\u7d50\u679c\u304c\u5f97\u3089\u308c\u307e\u3059\u3002poly(A)+ RNA\u3067\u306f\u3001\u5897\u5e45\u3055\u308c\u305fm &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[94],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/289"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=289"}],"version-history":[{"count":1,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/289\/revisions"}],"predecessor-version":[{"id":290,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/289\/revisions\/290"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=289"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=289"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=289"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}