{"id":296,"date":"2020-12-02T12:39:26","date_gmt":"2020-12-02T03:39:26","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=296"},"modified":"2020-12-23T16:21:07","modified_gmt":"2020-12-23T07:21:07","slug":"q388","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/genetic-reporter\/reporter-vector-and-cellline\/pgl4-vector\/q388","title":{"rendered":"pGL4.10[luc2] Vector\u306fpGL3-Basic Vector\u3068\u6bd4\u8f03\u3057\u3066\u3001\u3069\u306e\u3088\u3046\u306a\u7279\u5fb4\u304c\u3042\u308b\u306e\u3067\u3059\u304b\uff1f"},"content":{"rendered":"\n<ol><li>pGL4\u3067\u7528\u3044\u3089\u308c\u3066\u3044\u308bluc2\u907a\u4f1d\u5b50\u306f\u3001pGL3\u306eluc+\u907a\u4f1d\u5b50\u306b\u6bd4\u3079\u306690%\u4ee5\u4e0a\u306e\u8ee2\u5199\u56e0\u5b50\u7d50\u5408\u90e8\u4f4d\u304c\u9664\u53bb\u3055\u308c\u3066\u3044\u307e\u3059 (\u56f31)\u3002<br>\u203b \u30a2\u30df\u30ce\u9178\u914d\u5217\u306b\u306f\u5909\u5316\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/li><li>pGL4\u306e\u30d9\u30af\u30bf\u30fc\u30d0\u30c3\u30af\u30dc\u30fc\u30f3\u306f\u3001pGL3\u306e\u30d9\u30af\u30bf\u30fc\u30d0\u30c3\u30af\u30dc\u30fc\u30f3\u306b\u6bd4\u3079\u306678%\u306e\u8ee2\u5199\u56e0\u5b50\u7d50\u5408\u90e8\u4f4d\u304c\u9664\u53bb\u3055\u308c\u3066\u3044\u307e\u3059 (\u56f32)\u3002<br>\u203b 1\u304a\u3088\u30732\u306e\u6539\u826f\u306b\u3088\u308a\u3001\u975e\u7279\u7570\u7684\u306a\u8ee2\u5199\u304c\u6291\u5236\u3055\u308c\u3001\u512a\u308c\u305f\u30b7\u30b0\u30ca\u30eb\u30fb\u30ce\u30a4\u30ba\u6bd4\u3092\u5b9f\u73fe\u3057\u307e\u3059\u3002<\/li><li>\u54fa\u4e73\u52d5\u7269\u3067\u306e\u767a\u73fe\u306b\u6700\u9069\u5316\u3055\u308c\u305f\u30b3\u30c9\u30f3\u306b\u3088\u308a\u9ad8\u3044\u767a\u73fe\u52b9\u7387\u3092\u793a\u3057\u307e\u3059 (\u56f33)\u3002<\/li><li>\u30de\u30eb\u30c1\u30af\u30ed\u30fc\u30f3\u30b0\u30b5\u30a4\u30c8\u306b\u65b0\u3057\u3044\u5236\u9650\u9175\u7d20\u90e8\u4f4d\u3092\u4ed8\u52a0\u3057\u307e\u3057\u305f (\u56f34)\u3002<\/li><li>Hygromycin\u8010\u6027\u907a\u4f1d\u5b50\u3092\u542b\u3093\u3060\u5b89\u5b9a\u767a\u73fe\u682a\u4f5c\u6210\u7528\u30d9\u30af\u30bf\u30fc\u3092\u3054\u7528\u610f\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/li><\/ol>\n\n\n\n<p>pGL4.10[luc2] Vector\u3068pGL3-Basic Vector\u306e\u4e0a\u8a18\u306e\u76f8\u9055\u70b9\u3092\u307e\u3068\u3081\u305f\u88682\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<p><strong>\u88682. pGL4.10[luc2] Vector\u3068pGL3-Basic Vector\u306e\u76f8\u9055\u70b9<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><th>\u3000<\/th><th>pGL3<\/th><th>pGL4.10<\/th><\/tr><tr><td>\u30db\u30bf\u30eb\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u907a\u4f1d\u5b50\u306e\u540d\u79f0<\/td><td>luc+<\/td><td>luc2<\/td><\/tr><tr><td>\u54fa\u4e73\u52d5\u7269\u3067\u306e\u767a\u73fe\u306b\u6700\u9069\u5316\u3057\u305f\u30b3\u30c9\u30f3\u3078\u306e\u6539\u5909<\/td><td>\u25b3<\/td><td>\u25cb<\/td><\/tr><tr><td>\u4e88\u6e2c\u3055\u308c\u308b\u8ee2\u5199\u8abf\u7bc0\u56e0\u5b50\u7d50\u5408\u90e8\u4f4d\u306e\u9664\u53bb<\/td><td>\u306a\u3057<\/td><td>\u30db\u30bf\u30eb\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u907a\u4f1d\u5b50<br>\uff0b \u30d9\u30af\u30bf\u30fc\u30d0\u30c3\u30af\u30dc\u30fc\u30f3<\/td><\/tr><tr><td>\u5b89\u5b9a\u767a\u73fe\u682a\u4f5c\u6210\u7528\u30d9\u30af\u30bf\u30fc<\/td><td>\u306a\u3057<\/td><td>pGL4.14[luc2\/Hygro]<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><strong>\u56f31. luc+(pGL3)\u3068luc2(pGL4)\u306e\u8ee2\u5199\u56e0\u5b50\u7d50\u5408\u90e8\u4f4d<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"387\" height=\"332\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/img_pgl4_01.gif\" alt=\"\" class=\"wp-image-2059\"\/><\/figure>\n\n\n\n<p><strong>\u56f33. luc+(pGL3)\u3068luc2(pGL4)\u306e\u767a\u73fe\u52b9\u7387\u306e\u6bd4\u8f03<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"273\" height=\"401\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/img_pgl4_03.gif\" alt=\"\" class=\"wp-image-2062\"\/><\/figure>\n\n\n\n<p><strong>\u56f32. pGL3\u3068pGL4\u306e\u30d9\u30af\u30bf\u30fc\u30d0\u30c3\u30af\u30dc\u30fc\u30f3\u306b\u304a\u3051\u308b\u8ee2\u5199\u56e0\u5b50\u7d50\u5408\u90e8\u4f4d<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"557\" height=\"196\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/img_pgl4_02.gif\" alt=\"\" class=\"wp-image-2063\"\/><\/figure>\n\n\n\n<p><strong>\u56f34. \u30de\u30eb\u30c1\u30af\u30ed\u30fc\u30f3\u30b0\u30b5\u30a4\u30c8\u306b\u304a\u3051\u308b\u5236\u9650\u9175\u7d20\u90e8\u4f4d\u306e\u6bd4\u8f03<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"518\" height=\"196\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/img_pgl4_04.gif\" alt=\"\" class=\"wp-image-2064\"\/><\/figure>\n\n\n\n<p>\u203b pGL4.7\u30b7\u30ea\u30fc\u30ba\u3067\u306f\u3001hRluc\u907a\u4f1d\u5b50\u4e2d\u306bBglI\u3068EcoRV\u304c\u5b58\u5728\u3059\u308b\u305f\u3081\u3001\u3053\u308c\u3089\u306e\u5236\u9650\u9175\u7d20\u3092\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306b\u4f7f\u7528\u3059\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>pGL4\u3067\u7528\u3044\u3089\u308c\u3066\u3044\u308bluc2\u907a\u4f1d\u5b50\u306f\u3001pGL3\u306eluc+\u907a\u4f1d\u5b50\u306b\u6bd4\u3079\u306690%\u4ee5\u4e0a\u306e\u8ee2\u5199\u56e0\u5b50\u7d50\u5408\u90e8\u4f4d\u304c\u9664\u53bb\u3055\u308c\u3066\u3044\u307e\u3059 (\u56f31)\u3002\u203b \u30a2\u30df\u30ce\u9178\u914d\u5217\u306b\u306f\u5909\u5316\u306f\u3042\u308a\u307e\u305b\u3093\u3002 pGL4\u306e\u30d9\u30af\u30bf\u30fc\u30d0\u30c3\u30af\u30dc\u30fc\u30f3\u306f\u3001pGL3\u306e\u30d9\u30af &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[174],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/296"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=296"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/296\/revisions"}],"predecessor-version":[{"id":2065,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/296\/revisions\/2065"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=296"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=296"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=296"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}