{"id":315,"date":"2020-12-02T12:48:48","date_gmt":"2020-12-02T03:48:48","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=315"},"modified":"2020-12-15T15:48:34","modified_gmt":"2020-12-15T06:48:34","slug":"q106","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/amplification-quantification\/pcr-and-rt\/qpcr\/gotaq-qpcr-mastermix\/q106","title":{"rendered":"\u88dc\u6b63\u7528\u306e\u8272\u7d20\uff08CXR\uff09\u306e\u6dfb\u52a0\u304c\u5fc5\u8981\u306a\u6a5f\u5668\u306f\uff1f"},"content":{"rendered":"\n

\u88dc\u6b63\u7528\u8272\u7d20\u306eCXR\uff08carboxy-X-rhodamine\uff09\u306f\u3001ROX\u2122\u3068\u540c\u3058\u3088\u3046\u306b\u5229\u7528\u3057\u307e\u3059\u3002ROX\u306a\u3069\u30d1\u30c3\u30b7\u30d6\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u8272\u7d20\u306b\u3088\u308b\u88dc\u6b63\u304c\u5fc5\u8981\u306a\u6a5f\u5668\u306e\u306a\u304b\u306b\u306f\u3001\u4f4e\u6fc3\u5ea6ROX\u3082\u3057\u304f\u306f\u9ad8\u6fc3\u5ea6ROX\u3067\u88dc\u6b63\u3059\u308b\u6a5f\u5668\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n

\u86cd\u5149\u8272\u7d20\u30d9\u30fc\u30b9\u306eGoTaq qPCR Master Mix \uff08\u30ab\u30bf\u30ed\u30b0\u756a\u53f7A6001, A6002\uff09\u306b\u306f\u3001\u3042\u3089\u304b\u3058\u3081\u4f4e\u6fc3\u5ea6CXR\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u88dc\u6b63\u4e0d\u8981\u3082\u3057\u304f\u306f\u4f4e\u6fc3\u5ea6ROX\u88dc\u6b63\u6a5f\u5668\u306e\u5834\u5408\u3001CXR\u6dfb\u52a0\u306f\u4e0d\u8981\u3067\u3059\u3002\u9ad8\u6fc3\u5ea6ROX\u88dc\u6b63\u6a5f\u5668\u306e\u5834\u5408\u306b\u306f\u3001\u6700\u7d42\u6fc3\u5ea6300 nM\uff0820 \u00b5l\u306e\u53cd\u5fdc\u6db2\u306b\u5bfe\u3057\u3066CXR\u30920.2 \u00b5l\uff09\u306b\u306a\u308b\u3088\u3046\u306b\u52a0\u3048\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\u86cd\u5149\u30d7\u30ed\u30fc\u30d6\u30d9\u30fc\u30b9\u306eGoTaq Probe qPCR Master Mix \uff08\u30ab\u30bf\u30ed\u30b0\u756a\u53f7A6101, A6102\uff09\u306b\u306f\u3001\u3042\u3089\u304b\u3058\u3081CXR\u306f\u542b\u307e\u308c\u3066\u3044\u306a\u3044\u305f\u3081\u3001\u6a5f\u5668\u306b\u5fdc\u3058\u3066\u4ee5\u4e0b\u306e\u3088\u3046\u306bCXR\u3092\u6dfb\u52a0\u3057\u3054\u4f7f\u7528\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\u30fb\u4f4e\u6fc3\u5ea6ROX\u88dc\u6b63\u6a5f\u5668\u306e\u5834\u5408<\/strong>
1 ml GoTaq Probe qPCR Master Mix, 2X\u306b\u5bfe\u3057\u30012 \u00b5l CXR\u6dfb\u52a0
\u30fb\u9ad8\u6fc3\u5ea6ROX\u88dc\u6b63\u6a5f\u5668\u306e\u5834\u5408<\/strong>
1 ml GoTaq Probe qPCR Master Mix, 2X\u306b\u5bfe\u3057\u300133.4 \u00b5l CXR\u6dfb\u52a0
\u4e00\u89a7\u8868\u306b\u306a\u3044\u6a5f\u7a2e\u3084CXR\u6dfb\u52a0\u306e\u4e0d\u660e\u70b9\u306f\u3001\u5f0a\u793e\u5b66\u8853\u90e8\u307e\u3067\u304a\u554f\u3044\u5408\u308f\u305b\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\u30d1\u30c3\u30b7\u30d6\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u8272\u7d20\u306b\u3088\u308b\u88dc\u6b63\u4e0d\u8981\u6a5f\u5668<\/strong>
\u2022 Bio-Rad CFX96 Real-Time PCR Detection System
\u2022 Bio-Rad DNA Engine Opticon\u00ae<\/sup> and Opticon\u00ae<\/sup> 2 Real-Time PCR Detection Systems
\u2022 Bio-Rad\/MJ Research Chromo4\u2122 Real-Time Detector
\u2022 Bio-Rad iCycler iQ\u00ae<\/sup> and iQ\u00ae<\/sup>5 Real-Time PCR Detection Systems \u2022 Bio-Rad MyiQ\u2122 Real-Time PCR Detection System
\u2022 Roche LightCycler\u00ae<\/sup> 480 Real-Time PCR System
\u2022 Eppendorf Mastercycler\u00ae<\/sup> ep realplex Real-Time PCR System

\u4f4e\u6fc3\u5ea6\u30d1\u30c3\u30b7\u30d6\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u8272\u7d20\u306b\u3088\u308b\u88dc\u6b63\u6a5f\u5668<\/strong>
\u2022 Applied Biosystems 7500 and 7500 FAST Real-Time PCR System
\u2022 Applied Biosystems QuantStudio\u00ae<\/sup> Real Time PCR Systems
\u2022 Applied Biosystems ViiA\u00ae<\/sup> 7 Real-Time PCR System
\u2022 Stratagene\/Agilent Mx3000P\u00ae<\/sup> and Mx3005P\u00ae<\/sup> Real-Time PCR Systems
\u2022 Stratagene\/Agilent Mx4000\u00ae<\/sup> Multiplex Quantitative PCR System

\u9ad8\u6fc3\u5ea6\u30d1\u30c3\u30b7\u30d6\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u8272\u7d20\u306b\u3088\u308b\u88dc\u6b63\u6a5f\u5668<\/strong>
\u2022 Applied Biosystems StepOne\u2122 and StepOnePlus\u2122 Real-Time PCR Systems
\u2022 Applied Biosystems 7300 and 7900HT Real-Time PCR System<\/p>\n","protected":false},"excerpt":{"rendered":"

\u88dc\u6b63\u7528\u8272\u7d20\u306eCXR\uff08carboxy-X-rhodamine\uff09\u306f\u3001ROX\u2122\u3068\u540c\u3058\u3088\u3046\u306b\u5229\u7528\u3057\u307e\u3059\u3002ROX\u306a\u3069\u30d1\u30c3\u30b7\u30d6\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u8272\u7d20\u306b\u3088\u308b\u88dc\u6b63\u304c\u5fc5\u8981\u306a\u6a5f\u5668\u306e\u306a\u304b\u306b\u306f\u3001\u4f4e\u6fc3\u5ea6ROX\u3082\u3057\u304f\u306f\u9ad8\u6fc3\u5ea6ROX\u3067\u88dc\u6b63\u3059\u308b\u6a5f\u5668\u304c\u3042\u308a\u307e\u3059 … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[96],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/315"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=315"}],"version-history":[{"count":1,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/315\/revisions"}],"predecessor-version":[{"id":316,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/315\/revisions\/316"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=315"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=315"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=315"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}