{"id":327,"date":"2020-12-02T12:51:21","date_gmt":"2020-12-02T03:51:21","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=327"},"modified":"2021-01-22T11:55:55","modified_gmt":"2021-01-22T02:55:55","slug":"q109","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/amplification-quantification\/pcr-and-rt\/qpcr\/gotaq-qpcr-mastermix\/q109","title":{"rendered":"Fast Cycling Program\u306b\u5bfe\u5fdc\u53ef\u80fd\u3067\u3059\u304b\uff1f"},"content":{"rendered":"\n<p>\u5bfe\u5fdc\u53ef\u80fd\u3067\u3059\u3002\u6a19\u6e96\u30d7\u30ed\u30b0\u30e9\u30e0\u3068Fast Cycling\u30d7\u30ed\u30b0\u30e9\u30e0 \u306e\u4f8b\u3067\u3059\u3002<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td><\/td><td>#Cycles<\/td><td>Standard Cycling Program<\/td><td>Fast Cycling Program<\/td><\/tr><tr><td>Hot-Start Activation<\/td><td>1<\/td><td>95\u2103 for 2 minutes<\/td><td>95\u2103 for 2 minutes<\/td><\/tr><tr><td style=\"background:#ddd;\">Denaturation<\/td><td style=\"background:#ddd;\">40<\/td><td style=\"background:#ddd;\">95\u2103 for 15 seconds<\/td><td style=\"background:#ddd;\">95\u2103 for 3 seconds<\/td><\/tr><tr><td style=\"background:#ddd;\">Annealing\/Extension<\/td><td style=\"background:#ddd;\">60\u2103 for 60 seconds<\/td><td style=\"background:#ddd;\">60\u2103 for 30 seconds<\/td><td style=\"background:#ddd;\">60\u2103 for 30 seconds<\/td><\/tr><tr><td>Dissociation<\/td><td>1<\/td><td>60-95\u2103<\/td><td>60-95\u2103<\/td><\/tr><\/tbody><\/table><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>\u5bfe\u5fdc\u53ef\u80fd\u3067\u3059\u3002\u6a19\u6e96\u30d7\u30ed\u30b0\u30e9\u30e0\u3068Fast Cycling\u30d7\u30ed\u30b0\u30e9\u30e0 \u306e\u4f8b\u3067\u3059\u3002 #Cycles Standard Cycling Program Fast Cycling Program Hot-Start Activat &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[96],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/327"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=327"}],"version-history":[{"count":7,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/327\/revisions"}],"predecessor-version":[{"id":1855,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/327\/revisions\/1855"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=327"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=327"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=327"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}