{"id":3306,"date":"2022-03-22T15:19:14","date_gmt":"2022-03-22T06:19:14","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=3306"},"modified":"2022-03-22T16:31:15","modified_gmt":"2022-03-22T07:31:15","slug":"q911","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/cloning\/clone\/kazusa-clone\/q911","title":{"rendered":"ORF\u3092\u7d44\u307f\u8fbc\u3093\u3060\u3001\u5404\u30af\u30ed\u30fc\u30f3\u306e\u3082\u3068\u306e\u30d9\u30af\u30bf\u30fc\u306f\u3069\u308c\u3067\u3059\u304b\uff1f"},"content":{"rendered":"\u5404\u30af\u30ed\u30fc\u30f3\u306f\u4e0b\u8a18\u306e\u5f0a\u793e\u30d9\u30af\u30bf\u30fc\u3092\u3082\u3068\u306b\u3057\u3066\u3044\u307e\u3059\u3002\n<table style=\"border-collapse: collapse;width: 974px;height: 151px\" border=\"0\" width=\"838\" cellspacing=\"0\" cellpadding=\"0\"><!--StartFragment--> \n<colgroup>\n<col style=\"width: 188pt\" width=\"250\">\n<col style=\"width: 75pt\" width=\"100\">\n<col style=\"width: 73pt\" width=\"98\">\n<col style=\"width: 95pt\" width=\"127\">\n<col style=\"width: 197pt\" width=\"263\"> <\/colgroup>\n<tbody>\n<tr style=\"height: 18.0pt\">\n<td class=\"xl67\" style=\"height: 18.0pt;width: 188pt\" width=\"250\" height=\"24\">\u30bf\u30a4\u30d7\u5225<\/td>\n<td class=\"xl67\" style=\"border-left: none;width: 75pt\" width=\"100\">\u88fd\u54c1\u8a18\u53f7<\/td>\n<td class=\"xl67\" style=\"border-left: none;width: 73pt\" width=\"98\">Tag<\/td>\n<td class=\"xl67\" style=\"border-left: none;width: 95pt\" width=\"127\">Vector Backbone<\/td>\n<td class=\"xl67\" style=\"border-left: none;width: 197pt\" width=\"263\">\u85ac\u5264\u8010\u6027\u30ab\u30bb\u30c3\u30c8\uff08\u5927\u8178\u83cc\/\u52d5\u7269\u7d30\u80de\uff09\u4ed6<\/td>\n<\/tr>\n<tr style=\"height: 18.0pt\">\n<td class=\"xl65\" style=\"height: 18.0pt;border-top: none\" height=\"24\">Flexi\u00ae Cloning Type<\/td>\n<td class=\"xl65\" style=\"border-top: none;border-left: none\">FXC######<\/td>\n<td class=\"xl65\" style=\"border-top: none;border-left: none\">\u30bf\u30b0\u306a\u3057<\/td>\n<td class=\"xl66\" style=\"border-top: none;border-left: none\"><!-- \/wp:paragraph --><!-- wp:paragraph --><a href=\"https:\/\/www.promega.jp\/products\/cloning-and-dna-markers\/cloning-vectors-and-kits\/pf1-t7-flexi-vectors\/?catNum=C8451\">pF1K #C8451<\/a><\/td>\n<td class=\"xl65\" style=\"border-top: none;border-left: none;text-align: left\">KanR\/-&nbsp; &nbsp;\u203b1<\/td>\n<\/tr>\n<tr style=\"height: 18.0pt\">\n<td class=\"xl65\" style=\"height: 18.0pt;border-top: none\" height=\"24\">Flexi\u00ae HaloTag\u00ae Type<\/td>\n<td class=\"xl65\" style=\"border-top: none;border-left: none\">FHC######<\/td>\n<td class=\"xl65\" style=\"border-top: none;border-left: none\">N-HaloTag\u00ae&nbsp;<\/td>\n<td class=\"xl66\" style=\"border-top: none;border-left: none\"><!-- \/wp:paragraph --><!-- wp:paragraph --><a href=\"https:\/\/www.promega.jp\/products\/protein-detection\/protein-labeling\/pfn21a-and-pfn21k-halotag-cmv-flexi-vectors\/?catNum=G2821\">pFN21A #G2821<\/a><\/td>\n<td class=\"xl65\" style=\"border-top: none;border-left: none\">AmpR\/-\u3000\u203b\uff12<\/td>\n<\/tr>\n<tr style=\"height: 18.0pt\">\n<td class=\"xl65\" style=\"height: 18.0pt;border-top: none\" height=\"24\">Flexi\u00ae NanoLuc\u00ae Clone \uff08N\u672b\u7aef\u878d\u5408\uff09<\/td>\n<td class=\"xl65\" style=\"border-top: none;border-left: none\">FNC######N<\/td>\n<td class=\"xl65\" style=\"border-top: none;border-left: none\">N-NanoLuc\u00ae<\/td>\n<td class=\"xl66\" style=\"border-top: none;border-left: none\"><!-- \/wp:paragraph --><!-- wp:paragraph --><a href=\"https:\/\/www.promega.jp\/products\/luciferase-assays\/genetic-reporter-vectors-and-cell-lines\/pfn31-and-pfc32-nanoluc-protein-fusion-flexi-vectors\/?catNum=N1321\">pFN31K #N1321<\/a><\/td>\n<td class=\"xl65\" style=\"border-top: none;border-left: none\">KanR\/NeoR<\/td>\n<\/tr>\n<tr style=\"height: 18.0pt\">\n<td class=\"xl65\" style=\"height: 18.0pt;border-top: none\" height=\"24\">\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\uff08C\u672b\u7aef\u878d\u5408\uff09<\/td>\n<td class=\"xl65\" style=\"border-top: none;border-left: none\">FNC######C<\/td>\n<td class=\"xl65\" style=\"border-top: none;border-left: none\">C-NanoLuc\u00ae<\/td>\n<td class=\"xl66\" style=\"border-top: none;border-left: none\"><!-- \/wp:paragraph --><!-- wp:paragraph --><a href=\"https:\/\/www.promega.jp\/products\/luciferase-assays\/genetic-reporter-vectors-and-cell-lines\/pfn31-and-pfc32-nanoluc-protein-fusion-flexi-vectors\/?catNum=N1341\">pFC32K #N1341<\/a><!-- \/wp:paragraph --><\/td>\n<td class=\"xl65\" style=\"border-top: none;border-left: none\">KanR\/NeoR<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<span style=\"font-size: inherit\">\u203b\uff11\uff0e\u5927\u8178\u83cc\u767a\u73fe\u7528\u3001\u54fa\u4e73\u52d5\u7269\u767a\u73fe\u975e\u5bfe\u5fdc\u3002\n<\/span><span style=\"font-size: inherit\">\u30bf\u30b0\u306a\u3057\u306e\u54fa\u4e73\u52d5\u7269\u767a\u73fe\u7528\u30d0\u30c3\u30af\u30dc\u30fc\u30f3\u3068\u3057\u3066\u306f\u3001<\/span><a style=\"font-size: inherit\" href=\"https:\/\/www.promega.jp\/products\/cloning-and-dna-markers\/cloning-vectors-and-kits\/pf4-cmv-flexi-vectors\/?catNum=C8481\">pF4A (#C8481)<\/a><span style=\"font-size: inherit\">\u3084\u3001<\/span><a style=\"font-size: inherit\" href=\"https:\/\/www.promega.jp\/products\/cloning-and-dna-markers\/cloning-vectors-and-kits\/pf5-untagged-flexi-mammalian-expression-vectors\/?catNum=C9411\">pF5K CMV-neo(#C9411)<\/a><span style=\"font-size: inherit\"> \u7b49\u3092\u3054\u5229\u7528\u304f\u3060\u3055\u3044\u3002\n<\/span><span style=\"font-size: inherit\">\u203b\uff12\uff0e\uff23\u672b\u7aefHaloTag \u878d\u5408\u767a\u73fe\u306b\u306f\u3001<\/span><a style=\"font-size: inherit\" href=\"https:\/\/www.promega.jp\/products\/protein-detection\/protein-labeling\/pfc14a-and-pfc14k-halotag-cmv-flexi-vectors\/?catNum=G9661\">pFC14K #G9661<\/a><span style=\"font-size: inherit\"> \u4ed6\u3092\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002<\/span>\n\nFlexi(R) \u30b7\u30b9\u30c6\u30e0\u3092\u7528\u3044\u305fORF\u306e\u79fb\u3057\u66ff\u3048\u306b\u3064\u3044\u3066\u306f\u3001<a href=\"https:\/\/www.promega.co.jp\/resources\/faq\/cloning\/cloning-2\/system\/flexi-vector-system\/q812\">\u3053\u3061\u3089\u306eQ&amp;A<\/a>\u3092\u53c2\u7167\u304f\u3060\u3055\u3044\u3002\n\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n\n<!-- \/wp:paragraph -->","protected":false},"excerpt":{"rendered":"<p>\u5404\u30af\u30ed\u30fc\u30f3\u306f\u4e0b\u8a18\u306e\u5f0a\u793e\u30d9\u30af\u30bf\u30fc\u3092\u3082\u3068\u306b\u3057\u3066\u3044\u307e\u3059\u3002 \u30bf\u30a4\u30d7\u5225 \u88fd\u54c1\u8a18\u53f7 Tag Vector Backbone \u85ac\u5264\u8010\u6027\u30ab\u30bb\u30c3\u30c8\uff08\u5927\u8178\u83cc\/\u52d5\u7269\u7d30\u80de\uff09\u4ed6 Flexi\u00ae Cloning Type FXC###### \u30bf\u30b0\u306a\u3057 &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[113,125],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/3306"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=3306"}],"version-history":[{"count":11,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/3306\/revisions"}],"predecessor-version":[{"id":3320,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/3306\/revisions\/3320"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=3306"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=3306"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=3306"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}