{"id":358,"date":"2020-12-02T14:31:22","date_gmt":"2020-12-02T05:31:22","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=358"},"modified":"2020-12-28T11:12:37","modified_gmt":"2020-12-28T02:12:37","slug":"q394","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/genetic-reporter\/reporter-vector-and-cellline\/pgl4-vector\/q394","title":{"rendered":"\u4ed6\u306e\u30ec\u30dd\u30fc\u30bf\u30fc\u306b\u6bd4\u3079\u3066\u3001Rapid Response Reporter Vectors\u306e\u767a\u73fe\u91cf\u3068\u534a\u6e1b\u671f\u306f\u3069\u306e\u3088\u3046\u306b\u306a\u3063\u3066\u3044\u307e\u3059\u304b\uff1f"},"content":{"rendered":"\n<p>\u6bd4\u8f03\u8868\u3092\u4ee5\u4e0b\u306b\u793a\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<figure class=\"wp-block-table\">\n    <table>\n        <tbody>\n            <tr>\n                <th>\u3000<\/th>\n                <th colspan=\"2\">NanoLuc<\/th>\n                <th colspan=\"3\">\u30db\u30bf\u30eb \u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc<\/th>\n                <th colspan=\"3\">\u30a6\u30df\u30b7\u30a4\u30bf\u30b1 \u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc<\/th>\n            <\/tr>\n            <tr>\n                <td>\u30d9\u30af\u30bf\u30fc<\/td>\n                <td>pNL1.1<br>[Nluc]<\/td>\n                <td>pNL1.2<br>[NlucP]<\/td>\n                <td>pGL4.10<br>[luc2]<\/td>\n                <td>pGL4.11<br>[luc2P]<\/td>\n                <td>pGL4.12<br>[luc2CP]<\/td>\n                <td>pGL4.70<br>[hRluc]<\/td>\n                <td>pGL4.71<br>[hRlucP]<\/td>\n                <td>pGL4.72<br>[hRlucCP]<\/td>\n            <\/tr>\n            <tr>\n                <td>\u8a98\u5c0e\u500d\u7387\u304c\u6700\u5927\u306b<br>\u9054\u3059\u308b\u305f\u3081\u306b<br>\u5fc5\u8981\u306a\u6642\u9593<sup>\u203b1<\/sup>&nbsp;(hour)<\/td>\n                <td>2.5<\/td>\n                <td>2<\/td>\n                <td>4.5<\/td>\n                <td>4.5<\/td>\n                <td>3.0<\/td>\n                <td>6.0<\/td>\n                <td>4.0<\/td>\n                <td>2.0<\/td>\n            <\/tr>\n            <tr>\n                <td>\u523a\u6fc0\u304b\u30892\u6642\u9593\u5f8c\u306e<br>\u8a98\u5c0e\u500d\u7387<\/td>\n                <td>10\u500d<\/td>\n                <td>400\u500d<\/td>\n                <td>6.6\u500d<\/td>\n                <td>13.8\u500d<\/td>\n                <td>21.6\u500d<\/td>\n                <td>2.7\u500d<\/td>\n                <td>6.3\u500d<\/td>\n                <td>12.2\u500d<\/td>\n            <\/tr>\n            <tr>\n                <td>\u76f8\u5bfe\u7684\u306a\u767a\u5149\u30b7\u30b0\u30ca\u30eb\u306e<br>\u5f37\u5ea6<sup>\u203b2<\/sup><\/td>\n                <td>100%<\/td>\n                <td>1.2%<\/td>\n                <td>100%<\/td>\n                <td>18.7%<\/td>\n                <td>3.9%<\/td>\n                <td>100%<\/td>\n                <td>40%<\/td>\n                <td>5.8%<\/td>\n            <\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>\u203b1 : \u30db\u30bf\u30eb\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u306f\u56f31\u53c2\u7167\u3001\u30a6\u30df\u30b7\u30a4\u30bf\u30b1\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u306f\u56f32\u53c2\u7167<br>\u203b2 : \u56f33\u53c2\u7167<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"753\" height=\"355\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/reporter2_glaf_3.jpg\" alt=\"\" class=\"wp-image-2077\"\/><figcaption><strong>\u56f3. NanoLuc Rapid Response \u30bf\u30a4\u30d7\uff08NlucP\uff09\u306e\u5fdc\u7b54\u6027\u3002\uff08\u7e26\u8ef8\u306f\u5bfe\u6570\u30b9\u30b1\u30fc\u30eb\u3067\u3042\u308b\u3053\u3068\u306b\u6ce8\u610f\uff09<\/strong><\/figcaption><\/figure>\n\n\n\n<p>NF-\u03baB \u5fdc\u7b54\u30a8\u30ec\u30e1\u30f3\u30c8\u3092\u542b\u3080\u5404\u30d9\u30af\u30bf\u30fc\u3092HEK293\u306b\u5c0e\u5165\u3057\u3001TNF\u03b1\u3067\u51e6\u7406\u3057\u305f\u3002\u523a\u6fc0\u5f8c\u306e\u767a\u5149\u6bd4\u306e\u6642\u9593\u4f9d\u5b58\u6027\uff08\u5de6\u56f3\uff09\u3001\u307e\u305f\uff15\u6642\u9593\u5f8c\u306e\u5404\u6fc3\u5ea6\u306e\u523a\u6fc0\u306b\u5bfe\u3059\u308b\u5fdc\u7b54\u3092\u6e2c\u5b9a\u3057\u305f\u3002<br>\u523a\u6fc0\u524d\u5f8c\u3067\u767a\u5149\u5024\u306eS\/B\u6bd4\u306f\u3001NlucP \u304c\u3082\u3063\u3068\u3082\u3059\u3050\u308c\u3066\u3044\u305f\u3002Nluc\u306f\u305d\u308c\u81ea\u4f53\u304c\u5b89\u5b9a\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3042\u308b\u305f\u3081\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u304c\u4e0a\u6607\u3057\u305f\u304c\uff08\u53f3\u56f3Nluc\u8d64\uff09\u3001Rapid Response\u578b\u3067\u3053\u308c\u3092\u9632\u3052\u305f\uff08\u53f3\u56f3NlucP\u9752\uff09\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"400\" height=\"280\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/img_pgl4_10.gif\" alt=\"\" class=\"wp-image-2079\"\/><figcaption><strong>\u56f31. \u30db\u30bf\u30eb\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u907a\u4f1d\u5b50\u3092\u542b\u3080pGL4.1 Vector\u30b7\u30ea\u30fc\u30ba\u306b\u304a\u3051\u308b\u8a98\u5c0e\u500d\u7387<\/strong><\/figcaption><\/figure>\n\n\n\n<p>CRE (cAMP response element)\u3068HSV-TK\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3092\u633f\u5165\u3057\u305fpGL4 Vector\u3092HEK 293 cells\u306b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3057\u300124\u6642\u9593\u5f8c\u306b100\u03bcM RO (RO-20-1724)\u30681\u03bcM ISO (isoproterenol hydrochloride)\u3092\u6dfb\u52a0\u3057\u3001\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u306e\u767a\u73fe\u3092\u8a98\u5c0e\u3057\u305f\u3002\u307e\u305f\u3001100\u03bcM RO\u306e\u307f\u3092\u6dfb\u52a0\u3057\u305f\u3082\u306e\u3092\u975e\u8a98\u5c0e\u306e\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3068\u3057\u305f\u3002\u8a98\u5c0e\u5f8c1\u6642\u9593\u3054\u3068\u306b\u7d30\u80de\u3092\u56de\u53ce\u3057\u3001\u6eb6\u89e3\u3057\u305f\u30e9\u30a4\u30bb\u30fc\u30c8\u3092Luciferase Assay System (\u30ab\u30bf\u30ed\u30b0\u756a\u53f7 E1500)\u3067\u30a2\u30c3\u30bb\u30a4\u3057\u305f\u3002\u7e26\u8ef8\u306e\u5024\u306f\u3001\u2019\u8a98\u5c0e\u51e6\u7406\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u306e\u767a\u5149\u91cf\u2019\u306b\u5bfe\u3059\u308b\u2019\u672a\u51e6\u7406\u306e\u30b5\u30f3\u30d7\u30eb\u3067\u5f97\u3089\u308c\u305f\u767a\u5149\u91cf\u2019\u306e\u201d\u8a98\u5c0e\u500d\u7387\u201d\u3092\u8868\u3059(\u56f34\u53c2\u7167)\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"400\" height=\"300\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/img_pgl4_11.gif\" alt=\"\" class=\"wp-image-2080\"\/><figcaption><strong>\u56f32. \u30a6\u30df\u30b7\u30a4\u30bf\u30b1\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u907a\u4f1d\u5b50\u3092\u542b\u3080pGL4.7 Vector\u30b7\u30ea\u30fc\u30ba\u306b\u304a\u3051\u308b\u8a98\u5c0e\u500d\u7387<\/strong><\/figcaption><\/figure>\n\n\n\n<p>CRE (cAMP response element)\u3068HSV-TK\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3092\u633f\u5165\u3057\u305fpGL4 Vector\u3092HEK 293 cells\u306b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3057\u300124\u6642\u9593\u5f8c\u306b100\u03bcM RO (RO-20-1724)\u30681\u03bcM ISO (isoproterenol hydrochloride)\u3092\u6dfb\u52a0\u3057\u3001\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u306e\u767a\u73fe\u3092\u8a98\u5c0e\u3057\u305f\u3002\u307e\u305f\u3001100\u03bcM RO\u306e\u307f\u3092\u6dfb\u52a0\u3057\u305f\u3082\u306e\u3092\u975e\u8a98\u5c0e\u306e\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3068\u3057\u305f\u3002\u8a98\u5c0e\u5f8c1\u6642\u9593\u3054\u3068\u306b\u7d30\u80de\u3092\u56de\u53ce\u3057\u3001\u6eb6\u89e3\u3057\u305f\u30e9\u30a4\u30bb\u30fc\u30c8\u3092Renilla Luciferase Assay System (\u30ab\u30bf\u30ed\u30b0\u756a\u53f7 E2810)\u3067\u30a2\u30c3\u30bb\u30a4\u3057\u305f\u3002\u7e26\u8ef8\u306e\u5024\u306f\u3001\u2019\u8a98\u5c0e\u51e6\u7406\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u306e\u767a\u5149\u91cf\u2019\u306b\u5bfe\u3059\u308b\u2019\u672a\u51e6\u7406\u306e\u30b5\u30f3\u30d7\u30eb\u3067\u5f97\u3089\u308c\u305f\u767a\u5149\u91cf\u2019\u306e\u201d\u8a98\u5c0e\u500d\u7387\u201d\u3092\u8868\u3059\u3002\u7e26\u8ef8\u306e\u201d\u8a98\u5c0e\u500d\u7387\u201d\u306f\u3001\u2019\u8a98\u5c0e\u51e6\u7406\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u306e\u767a\u5149\u91cf\u2019\u306b\u5bfe\u3059\u308b\u2019\u672a\u51e6\u7406\u306e\u30b5\u30f3\u30d7\u30eb\u3067\u5f97\u3089\u308c\u305f\u767a\u5149\u91cf\u2019\u306e\u5024\u3092\u8868\u3059(\u56f34\u53c2\u7167)\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"420\" height=\"270\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/img_pgl4_12.gif\" alt=\"\" class=\"wp-image-2081\"\/><figcaption><strong>\u56f33. pGL4.1 Vectors\u304a\u3088\u3073pGL4.7 Vectors\u306e\u9593\u306b\u304a\u3051\u308b\u767a\u73fe\u91cf\u306e\u6bd4\u8f03<\/strong><\/figcaption><\/figure>\n\n\n\n<p>CRE (cAMP response element)\u3068HSV-TK\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3092\u633f\u5165\u3057\u305fpGL4 Reporter Vector (\u30db\u30bf\u30eb\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc : pGL4.10[ luc2], pGL4.11[ luc2P], pGL4.12[ luc2CP] \u304a\u3088\u3073 \u30a6\u30df\u30b7\u30a4\u30bf\u30b1\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc : pGL4.70[ hRluc], pGL4.71[ hRlucP], pGL4.72[ hRlucCP])\u3092\u5185\u90e8\u6a19\u6e96\u7528\u30d9\u30af\u30bf\u30fc\u3068\u5171\u306bHEK 293 cells\u306b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3057\u305f\u3002\u30c8\u30e9\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u304b\u308924\u6642\u9593\u5f8c\u306b\u7d30\u80de\u3092\u56de\u53ce\u3057\u3001Passive Lysis Buffer\u3067\u6eb6\u89e3\u3057\u3001Dual-Luciferase<sup>\u00ae<\/sup>&nbsp;Reporter Assay System\u3067\u767a\u5149\u91cf\u3092\u6e2c\u5b9a\u3057\u305f\u3002\u5185\u90e8\u6a19\u6e96\u5024\u3067\u88dc\u6b63\u3057\u305f\u5024\u3092\u7e26\u8ef8\u306b\u30d7\u30ed\u30c3\u30c8\u3057\u305f\u3002<br><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u6bd4\u8f03\u8868\u3092\u4ee5\u4e0b\u306b\u793a\u3057\u307e\u3059\u3002 \u3000 NanoLuc \u30db\u30bf\u30eb \u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc \u30a6\u30df\u30b7\u30a4\u30bf\u30b1 \u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc \u30d9\u30af\u30bf\u30fc pNL1.1[Nluc] pNL1.2[NlucP] pGL4.10[luc2] pGL4.11[luc2P] &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[174],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/358"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=358"}],"version-history":[{"count":4,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/358\/revisions"}],"predecessor-version":[{"id":2082,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/358\/revisions\/2082"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=358"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=358"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=358"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}