{"id":375,"date":"2020-12-02T14:34:46","date_gmt":"2020-12-02T05:34:46","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=375"},"modified":"2020-12-28T11:19:11","modified_gmt":"2020-12-28T02:19:11","slug":"q395","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/genetic-reporter\/reporter-vector-and-cellline\/pgl4-vector\/q395","title":{"rendered":"pGL4.7 Vectors \u306fpRL Vectors\u3001phRL Vectors\u3068\u6bd4\u8f03\u3057\u3066\u3001\u3069\u306e\u3088\u3046\u306a\u7279\u5fb4\u304c\u3042\u308b\u306e\u3067\u3059\u304b\uff1f"},"content":{"rendered":"\n

pRL Vector\u3084\uff50\uff48RL Vector\u306b\u5bfe\u3057\u3066\u3001pGL4.7 Vector\u3067\u306f\u3001\u4ee5\u4e0b\u306b\u793a\u3059\u3088\u3046\u306a\u6539\u826f\u304c\u306a\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n

  1. pGL4\u3067\u7528\u3044\u3089\u308c\u3066\u3044\u308bhRluc\u907a\u4f1d\u5b50\u306f\u3001pRL\u306eRluc\u907a\u4f1d\u5b50\u306b\u6bd4\u3079\u306690%\u4ee5\u4e0a\u306e\u8ee2\u5199\u56e0\u5b50\u7d50\u5408\u90e8\u4f4d\u304c\u9664\u53bb\u3055\u308c\u3066\u3044\u307e\u3059 (\u56f35)\u3002
    \u203b \u30a2\u30df\u30ce\u9178\u914d\u5217\u306b\u306f\u5909\u5316\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/li>
  2. pGL4\u306e\u30d9\u30af\u30bf\u30fc\u30d0\u30c3\u30af\u30dc\u30fc\u30f3\u306f\u3001pGL3\u306e\u30d9\u30af\u30bf\u30fc\u30d0\u30c3\u30af\u30dc\u30fc\u30f3\u306b\u6bd4\u3079\u306678%\u306e\u8ee2\u5199\u56e0\u5b50\u7d50\u5408\u90e8\u4f4d\u304c\u9664\u53bb\u3055\u308c\u3066\u3044\u307e\u3059 (\u56f32)
    \u203b 1\u304a\u3088\u30732\u306e\u6539\u826f\u306b\u3088\u308a\u3001\u975e\u7279\u7570\u7684\u306a\u8ee2\u5199\u304c\u6291\u5236\u3055\u308c\u3001\u512a\u308c\u305f\u30b7\u30b0\u30ca\u30eb\u30fb\u30ce\u30a4\u30ba\u6bd4\u3092\u5b9f\u73fe\u3057\u307e\u3059\u3002<\/li>
  3. \u54fa\u4e73\u52d5\u7269\u3067\u306e\u767a\u73fe\u306b\u6700\u9069\u5316\u3055\u308c\u305f\u30b3\u30c9\u30f3\u306b\u3088\u308a\u9ad8\u3044\u767a\u73fe\u52b9\u7387\u3092\u793a\u3057\u307e\u3059 (\u56f36)\u3002<\/li>
  4. Hygromycin\u8010\u6027\u907a\u4f1d\u5b50\u3092\u542b\u3093\u3060\u5b89\u5b9a\u767a\u73fe\u682a\u4f5c\u6210\u7528\u30d9\u30af\u30bf\u30fc\u3092\u767a\u58f2\u3057\u307e\u3057\u305f\u3002<\/li><\/ol>\n\n\n\n

    pRL Vector\u3001phRL Vector\u3001pGL4.70[hRluc] Vector\u306e\u76f8\u9055\u70b9\u3092\u307e\u3068\u3081\u305f\u88685\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

    \u88685. \u5404\u30a6\u30df\u30b7\u30a4\u30bf\u30b1\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306e\u6bd4\u8f03<\/strong><\/p>\n\n\n\n

    \u3000<\/th>pRL<\/th>phRL<\/th>pGL4.70[hRluc]<\/th><\/tr>
    \u30a6\u30df\u30b7\u30a4\u30bf\u30b1\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u907a\u4f1d\u5b50\u306e\u540d\u79f0<\/td>Rluc(native)<\/td>hRluc<\/td>hRluc<\/td><\/tr>
    \u54fa\u4e73\u52d5\u7269\u3067\u306e\u767a\u73fe\u306b\u6700\u9069\u5316\u3057\u305f\u30b3\u30c9\u30f3\u3078\u306e\u6539\u5909<\/td>\u00d7<\/td>\u25cb<\/td>\u25cb<\/td><\/tr>
    \u4e88\u6e2c\u3055\u308c\u308b\u8ee2\u5199\u8abf\u7bc0\u56e0\u5b50\u7d50\u5408\u90e8\u4f4d\u306e\u9664\u53bb<\/td>\u306a\u3057<\/td>\u30a6\u30df\u30b7\u30a4\u30bf\u30b1\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u907a\u4f1d\u5b50\u306e\u307f<\/td>\u30a6\u30df\u30b7\u30a4\u30bf\u30b1\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u907a\u4f1d\u5b50 \uff0b \u30d9\u30af\u30bf\u30fc\u30d0\u30c3\u30af\u30dc\u30fc\u30f3<\/td><\/tr>
    \u5b89\u5b9a\u767a\u73fe\u682a\u4f5c\u6210\u7528\u30d9\u30af\u30bf\u30fc<\/td>\u306a\u3057<\/td>\u306a\u3057<\/td>pGL4.76
    [hRluc\/Hygro]<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
    \"\"
    \u56f35. hRluc(pGL4\u3068phRL)\u3068Rluc(pRL)\u306e\u8ee2\u5199\u56e0\u5b50\u7d50\u5408\u90e8\u4f4d<\/strong><\/figcaption><\/figure>\n\n\n\n
    \"\"
    \u56f36. hRluc(pGL4\u3068phRL)\u3068Rluc(pRL)\u306e\u767a\u73fe\u52b9\u7387\u306e\u6bd4\u8f03<\/strong><\/figcaption><\/figure>\n","protected":false},"excerpt":{"rendered":"

    pRL Vector\u3084\uff50\uff48RL Vector\u306b\u5bfe\u3057\u3066\u3001pGL4.7 Vector\u3067\u306f\u3001\u4ee5\u4e0b\u306b\u793a\u3059\u3088\u3046\u306a\u6539\u826f\u304c\u306a\u3055\u308c\u3066\u3044\u307e\u3059\u3002 pGL4\u3067\u7528\u3044\u3089\u308c\u3066\u3044\u308bhRluc\u907a\u4f1d\u5b50\u306f\u3001pRL\u306eRluc\u907a\u4f1d\u5b50\u306b\u6bd4\u3079\u306690%\u4ee5\u4e0a\u306e\u8ee2\u5199\u56e0\u5b50 … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[174],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/375"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=375"}],"version-history":[{"count":4,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/375\/revisions"}],"predecessor-version":[{"id":2087,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/375\/revisions\/2087"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=375"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=375"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=375"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}