{"id":420,"date":"2020-12-02T15:10:14","date_gmt":"2020-12-02T06:10:14","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=420"},"modified":"2020-12-28T11:31:43","modified_gmt":"2020-12-28T02:31:43","slug":"q400","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/genetic-reporter\/reporter-vector-and-cellline\/pgl4-vector\/q400","title":{"rendered":"minP\u5c0e\u5165\u6e08\u307f\u30d9\u30af\u30bf\u30fcpGL4\u30b7\u30ea\u30fc\u30ba\u306f\u3001\u3069\u306e\u3088\u3046\u306a\u88fd\u54c1\u304c\u3042\u308a\u307e\u3059\u304b\uff1f"},"content":{"rendered":"\n

\u8868\uff11\u306b\u30d9\u30af\u30bf\u30fc\u306e\u7279\u9577\u3068\u7528\u9014\u3092\u793a\u3057\u307e\u3059\u3002\u30d9\u30af\u30bf\u30fc\u9078\u629e\u306e\u53c2\u8003\u306b\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\u88681. minP\u5c0e\u5165\u6e08\u307fpGL4 \u30d9\u30af\u30bf\u30fc\u30b7\u30ea\u30fc\u30ba\u306e\u7279\u9577\u3068\u7528\u9014<\/strong><\/p>\n\n\n\n

\n \n \n \n \n \n
\u88fd\u54c1\u540d
(\u30ab\u30bf\u30ed\u30b0\u756a\u53f7)<\/th>\n 		pGL4.23
[luc2\/minP]
(E8411)<\/th>\n 		pGL4.24
[luc2P\/minP]
(E8421)<\/th>\n 		pGL4.25
[luc2CP\/minP]
(E8431)<\/th>\n 		pGL4.26
[luc2\/minP\/
Hygro]
(E8441)<\/th>\n 		pGL4.27
[luc2P\/minP\/
Hygro]
(E8451)<\/th>\n 		pGL4.28
[luc2CP\/minP\/
Hygro]
(E8561)<\/th>\n <\/tr>\n
\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\/
\u5fdc\u7b54\u914d\u5217<\/td>\n 		minP<\/td>\n minP<\/td>\n minP<\/td>\n minP<\/td>\n minP<\/td>\n minP<\/td>\n <\/tr>\n
\u30bf\u30f3\u30d1\u30af\u8cea
\u5206\u89e3\u914d\u5217<\/td>\n 		\u306a\u3057<\/td>\n hPEST<\/td>\n hCL1 + hPEST<\/td>\n \u306a\u3057<\/td>\n hPEST<\/td>\n hCL1 + hPEST<\/td>\n <\/tr>\n
\u85ac\u5264\u9078\u629e
\u30de\u30fc\u30ab\u30fc<\/td>\n 		\u306a\u3057<\/td>\n \u306a\u3057<\/td>\n \u306a\u3057<\/td>\n \u3042\u308a
(Hygromycin)<\/td>\n 		\u3042\u308a
(Hygromycin)<\/td>\n 		\u3042\u308a
(Hygromycin)<\/td>\n <\/tr>\n
\u7528\u9014<\/td>\n \u4e00\u822c\u7684\u306a
\u30ec\u30dd\u30fc\u30bf\u30fc\u30a2\u30c3\u30bb\u30a4<\/td>\n 		\u77ed\u6642\u9593\u306b\u6700\u5927\u306e\u8a98\u5c0e\u500d\u7387\u3092\u5f97\u308b\u5fc5\u8981\u304c\u3042\u308b
\u30ec\u30dd\u30fc\u30bf\u30fc\u30a2\u30c3\u30bb\u30a4*<\/td>\n 		\u5b89\u5b9a\u767a\u73fe\u682a\u306e\u4f5c\u6210<\/td>\n <\/tr>\n <\/tbody>\n <\/table>\n<\/figure>\n\n\n\n

* luc2CP\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u914d\u5217\u3067\u3042\u308bCL1\u3068PEST\u3092\u542b\u3080\u305f\u3081\u3001luc2\u3088\u308a\u3082\u4f4e\u3044\u767a\u5149\u5024\u3092\u793a\u3057\u307e\u3059\u3002<\/p>\n\n\n\n

\u88682. \u8ee2\u5199\u56e0\u5b50\u5fdc\u7b54\u914d\u5217\u304a\u3088\u3073minP\u5c0e\u5165\u6e08\u307fpGL4\u30d9\u30af\u30bf\u30fc\u30b7\u30ea\u30fc\u30ba\u306e\u7279\u9577\u3068\u7528\u9014<\/strong><\/p>\n\n\n\n

\n \n \n \n \n \n \n \n
\u88fd\u54c1\u540d
(\u30ab\u30bf\u30ed\u30b0\u756a\u53f7)<\/th>\n 		pGL4.29
[luc2P\/CRE\/Hygro]
(E8471)<\/th>\n 		pGL4.30
[luc2P\/NFAT-RE\/Hygro]
(E8481)<\/th>\n 		pGL4.32
[luc2P\/NF-kB-RE\/Hygro]
(E8491)<\/th>\n <\/tr>\n
\u5fdc\u7b54\u914d\u5217
\u914d\u5217
\uff08\u7e70\u308a\u8fd4\u3057\u56de\u6570\uff09<\/td>\n 		CRE
5′-\uff34\uff27\uff21\uff23\uff27\uff34\uff23\uff21-3′
( 3\u56de )<\/td>\n 		NFAT-RE
5′-GAAAA-3′
( 3\u56de )<\/td>\n 		NF-kB-RE
5′-GG A( A\/C) TTTCC-3′
( 5\u56de )<\/td>\n <\/tr>\n
\u30bf\u30f3\u30d1\u30af\u8cea
\u5206\u89e3\u914d\u5217<\/td>\n 		hPEST<\/td>\n hPEST<\/td>\n hPEST<\/td>\n <\/tr>\n
\u85ac\u5264\u9078\u629e
\u30de\u30fc\u30ab\u30fc<\/td>\n 		\u3042\u308a
(Hygromycin)<\/td>\n 		\u3042\u308a
(Hygromycin)<\/td>\n 		\u3042\u308a
(Hygromycin)<\/td>\n <\/tr>\n
\u7528\u9014<\/td>\n GPCR\u3092\u4ecb\u3057\u305f\u30b7\u30b0\u30ca\u30eb\u7d4c\u8def\u306e\u7814\u7a76<\/td>\n \u30b9\u30c8\u30ec\u30b9\u3001\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9
\u30b7\u30b0\u30ca\u30eb\u7d4c\u8def\u306e\u7814\u7a76<\/td>\n <\/tr>\n <\/tbody>\n <\/table>\n<\/figure>\n\n\n\n

\u3059\u3079\u3066\u306e\u30d9\u30af\u30bf\u30fc\u306f\u3001\u52d5\u7269\u7d30\u80de\u7528\u85ac\u5264\u9078\u629e\u30de\u30fc\u30ab\u30fc\u304c\u542b\u307e\u308c\u3066\u3044\u308b\u305f\u3081\u5b89\u5b9a\u767a\u73fe\u682a\u4f5c\u6210\u306b\u3082\u5229\u7528\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u8868\uff11\u306b\u30d9\u30af\u30bf\u30fc\u306e\u7279\u9577\u3068\u7528\u9014\u3092\u793a\u3057\u307e\u3059\u3002\u30d9\u30af\u30bf\u30fc\u9078\u629e\u306e\u53c2\u8003\u306b\u3057\u3066\u304f\u3060\u3055\u3044\u3002 \u88681. minP\u5c0e\u5165\u6e08\u307fpGL4 \u30d9\u30af\u30bf\u30fc\u30b7\u30ea\u30fc\u30ba\u306e\u7279\u9577\u3068\u7528\u9014 \u88fd\u54c1\u540d(\u30ab\u30bf\u30ed\u30b0\u756a\u53f7) pGL4.23[luc2\/minP](E8411) pGL … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[174],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/420"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=420"}],"version-history":[{"count":3,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/420\/revisions"}],"predecessor-version":[{"id":2094,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/420\/revisions\/2094"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=420"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=420"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=420"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}