{"id":441,"date":"2020-12-02T15:19:10","date_gmt":"2020-12-02T06:19:10","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=441"},"modified":"2020-12-16T16:49:37","modified_gmt":"2020-12-16T07:49:37","slug":"q154","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/cloning\/cloning-2\/vector\/pgem-t-vector-system\/q154","title":{"rendered":"\u3069\u306e\u3088\u3046\u306a\u8981\u56e0\u304c\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u52b9\u7387\u306b\u5f71\u97ff\u3057\u307e\u3059\u304b?"},"content":{"rendered":"\n<p>\u5869\u985e\u3001dNTPs\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u3001\u8010\u71b1\u6027\u9175\u7d20\u306e\u3088\u3046\u306a\u4e00\u822c\u7684\u306aPCR\u53cd\u5fdc\u6db2\u306b\u542b\u307e\u308c\u308b\u6210\u5206\u306f\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u52b9\u7387\u306b\u30de\u30a4\u30ca\u30b9\u8981\u56e0\u3068\u306a\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u524d\u306b\u3001\u3053\u308c\u3089\u306e\u6210\u5206\u3092\u30d7\u30ed\u30e1\u30ac\u306eWizard<sup>\u00ae<\/sup>&nbsp;PCR Preps DNA Purification System<sup>(e)<\/sup>\u3001\u30b5\u30a4\u30ba\u9664\u53bb\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u3001\u305d\u306e\u4ed6\u306e\u65b9\u6cd5(1)\u3092\u7528\u3044\u3066\u9664\u304f\u3053\u3068\u306b\u3088\u308a\u52b9\u7387\u306f\u4e0a\u304c\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u76ee\u7684\u306ePCR\u7523\u7269\u3068\u540c\u69d8\u306b\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u3084\u975e\u7279\u7570\u7684\u306b\u5897\u5e45\u3055\u308c\u305f\u7523\u7269\u30823\u2019\u672b\u7aef\u306bA\u306e\u7a81\u51fa\u3092\u542b\u3093\u3067\u3044\u308b\u306e\u3067\u3001T-Vector\u306b\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u3055\u308c\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u4f59\u5206\u306a\u7523\u7269\u3092\u9664\u304f\u3053\u3068\u3067\u76ee\u7684\u306e\u30a4\u30f3\u30b5\u30fc\u30c8\u3092\u542b\u3093\u3060\u767d\u3044\u30b3\u30ed\u30cb\u30fc\u306e\u51fa\u73fe\u78ba\u7387\u3092\u4e0a\u3052\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u3069\u306e\u3088\u3046\u306a\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u53cd\u5fdc\u3067\u3082\u6700\u7d42\u7684\u306b\u306f\u30d9\u30af\u30bf\u30fc\u3068\u30a4\u30f3\u30b5\u30fc\u30c8DNA\u306e\u30e2\u30eb\u6bd4\u3092\u5909\u3048\u3066\u6700\u9069\u5316\u3092\u884c\u3044\u307e\u3059\u3002\u901a\u5e38\u3067\u306f\u30a4\u30f3\u30b5\u30fc\u30c8\u3068\u30d9\u30af\u30bf\u30fc\u306e\u30e2\u30eb\u6bd4\u30921:3\u30011:1\u30013:1\u306e3\u7a2e\u985e\u3067\u884c\u3044\u3001\u6700\u9069\u5316\u3055\u308c\u305f\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u52b9\u7387\u3092\u30c6\u30b9\u30c8\u3057\u307e\u3059\u3002\u307e\u308c\u306binsert:vector\u306e\u30e2\u30eb\u6bd410:1\u307e\u3067\u306e\u30c6\u30b9\u30c8\u304c\u5fc5\u8981\u306a\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\uff08PN68-Q&amp;A)<\/p>\n\n\n\n<p><strong>\u53c2\u8003\u6587\u732e\uff1a<\/strong><br>1.Frohman, M.A. (1994) In: The Polymerase Chain Reaction, Mullis, K.B., Ferr\u30fb F. and Gibbs, R.A., eds. Birh\u822bser Boston, Cambridge, MA<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5869\u985e\u3001dNTPs\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u3001\u8010\u71b1\u6027\u9175\u7d20\u306e\u3088\u3046\u306a\u4e00\u822c\u7684\u306aPCR\u53cd\u5fdc\u6db2\u306b\u542b\u307e\u308c\u308b\u6210\u5206\u306f\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u52b9\u7387\u306b\u30de\u30a4\u30ca\u30b9\u8981\u56e0\u3068\u306a\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u524d\u306b\u3001\u3053\u308c\u3089\u306e\u6210\u5206\u3092\u30d7\u30ed\u30e1\u30ac\u306eWizard\u00ae&nbsp;PCR Pr &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[123],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/441"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=441"}],"version-history":[{"count":1,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/441\/revisions"}],"predecessor-version":[{"id":442,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/441\/revisions\/442"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=441"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=441"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=441"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}