{"id":443,"date":"2020-12-02T15:21:58","date_gmt":"2020-12-02T06:21:58","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=443"},"modified":"2020-12-21T11:29:11","modified_gmt":"2020-12-21T02:29:11","slug":"q155","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/cloning\/cloning-2\/vector\/pgem-t-vector-system\/q155","title":{"rendered":"T-Vector\u306e\u7dcf\u5408\u7684\u306a\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306e\u52b9\u7387\u306b\u5f71\u97ff\u3059\u308b\u305d\u306e\u4ed6\u306e\u8981\u56e0\u306f\u4f55\u3067\u3059\u304b?"},"content":{"rendered":"\n

\u3042\u308b\u30a4\u30f3\u30b5\u30fc\u30c8\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306e\u52b9\u7387\u3092\u4e88\u6e2c\u3059\u308b\u3053\u3068\u306f\u4e0d\u53ef\u80fd\u3067\u3059\u3002\u305d\u306e\u52b9\u7387\u306f\u30a4\u30f3\u30b5\u30fc\u30c8\u306e\u30b5\u30a4\u30ba\u3001\u914d\u5217\u3001PCR\u7523\u7269\u306e\u7cbe\u88fd\u5ea6\u306a\u3069\u306e\u591a\u304f\u306e\u8981\u56e0\u306b\u4f9d\u5b58\u3059\u308b\u305f\u3081\u3067\u3059\u3002\u3053\u308c\u3089\u306e\u8981\u56e0\u306f\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u306e\u52b9\u7387\u3068\u4e26\u3073\u3001T-Vector\u3078\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3092\u6210\u529f\u3055\u305b\u308b\u305f\u3081\u306b\u91cd\u8981\u3067\u3059\u3002\u4f8b\u3048\u3070\u3001\u5927\u304d\u306a\u30b5\u30a4\u30ba\u306e\u30a4\u30f3\u30b5\u30fc\u30c8\u3067\u306f\u6b20\u5931\u3084\u518d\u69cb\u6210\u304c\u8d77\u3053\u308a\u3084\u3059\u3044\u305f\u3081\u3001\u76ee\u7684\u306e\u30a4\u30f3\u30b5\u30fc\u30c8\u304c\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3055\u308c\u308b\u52b9\u7387\u306f\u6e1b\u5c11\u3057\u307e\u3059(\u6ce8\u610f\uff1a\u30d7\u30ed\u30e1\u30ac\u306epGEM\u00ae<\/sup>-T\u304a\u3088\u3073pGEM\u00ae<\/sup>-T Easy Vector System\u3067\u306f7.5kb\u307e\u3067\u306e\u30a4\u30f3\u30b5\u30fc\u30c8\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306b\u6210\u529f\u3057\u3066\u3044\u307e\u3059)\u3002\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3055\u308c\u306b\u304f\u3044\u30a4\u30f3\u30b5\u30fc\u30c8\u3067\u306f\u3001\u4e00\u822c\u7684\u306bDNA\u306e\u518d\u69cb\u6210\u3092\u6700\u4f4e\u9650\u306b\u6291\u3048\u308b\u305f\u3081\u306b\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u305f\u682a\u3092\u4f7f\u3046\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002\u3055\u3089\u306b\u3001\u5c0f\u3055\u306a\u30a4\u30f3\u30b5\u30fc\u30c8\u3067\u306flacZ gene\u306e\u30ea\u30fc\u30c7\u30a3\u30f3\u30b0\u30d5\u30ec\u30fc\u30e0\u3092\u7834\u58ca\u3067\u304d\u306a\u3044\u305f\u3081\u306b\u3001\u30b3\u30ed\u30cb\u30fc\u304c\u767d\u8272\u3092\u5448\u3057\u306a\u3044\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u5834\u5408\u3001\u51fa\u73fe\u3057\u305f\u9752\u3044\u30b3\u30ed\u30cb\u30fc\u306e\u591a\u304f\u306f\u76ee\u7684\u306e\u30a4\u30f3\u30b5\u30fc\u30c8\u3092\u542b\u3093\u3060\u30ea\u30b3\u30f3\u30d3\u30ca\u30f3\u30c8\u306e\u30d9\u30af\u30bf\u30fc\u3067\u3042\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u306e\u9752\u3044\u30b3\u30ed\u30cb\u30fc\u3088\u308a\u3082\u30b5\u30f3\u30d7\u30eb\u306e\u65b9\u3067\u3088\u308a\u591a\u304f\u306e\u9752\u3044\u30b3\u30ed\u30cb\u30fc\u304c\u73fe\u308c\u3066\u3044\u308b\u5834\u5408\u3001\u3053\u306e\u3088\u3046\u306a\u4e8b\u8c61\u304c\u8d77\u3053\u3063\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/p>\n\n\n\n

\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u304c\u6df7\u5165\u3057\u3066\u3044\u308b\u3068\u3001PCR\u7523\u7269\u306e\u7cbe\u88fd\u5ea6\u306f\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306e\u52b9\u7387\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u307e\u3059\u3002\u30d9\u30af\u30bf\u30fc\u306e\u201dT\u201d\u306e\u7a81\u51fa\u3068PCR\u7523\u7269\u306e\u201dA\u201d\u306e\u7a81\u51fa\u304c\u6b20\u5931\u3057\u305f\u5834\u5408\u3001\u5e73\u6ed1\u672b\u7aef\u306e\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u306b\u306a\u308b\u305f\u3081\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306e\u52b9\u7387\u306f\u8457\u3057\u304f\u4f4e\u4e0b\u3057\u307e\u3059\u3002\u8010\u71b1\u6027DNA polymerase\u304c3\u2019\u21925\u2019 \u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027(\u30d7\u30eb\u30fc\u30d5\u30ea\u30fc\u30c7\u30a3\u30f3\u30b0\u6d3b\u6027\uff1b\u88681)\u3092\u793a\u3059\u5834\u5408\u3001\u307b\u3068\u3093\u3069\u306ePCR\u7523\u7269\u304c\u5e73\u6ed1\u672b\u7aef\u306b\u306a\u308a\u307e\u3059\u3002\u4e0b\u8a18\u306e\u6587\u732e2\u306b\u304a\u3044\u3066\u201dA\u201d\u306e\u7a81\u51fa\u3092\u4ed8\u52a0\u3059\u308b\u305f\u3081\u306e\u7c21\u5358\u306aA-tailing\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u3092\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u3002pGEMR<\/sup>-T\u307e\u305f\u306fpGEMR<\/sup>-T Easy Vector System\u306b\u6dfb\u4ed8\u3055\u308c\u308b\u53d6\u308a\u6271\u3044\u8aac\u660e\u66f8Technical Manual TM042\u306b\u306f\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306e\u52b9\u7387\u306b\u3064\u3044\u3066\u306e\u91cd\u8981\u306a\u77e5\u898b\u304c\u8a73\u7d30\u306b\u8a18\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\u3002\uff08PN68-Q&A)<\/p>\n\n\n\n

\u88681.\u8010\u71b1\u6027\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u6bd4\u8f03\uff081\uff09<\/p>\n\n\n\n

\u7279\u9577<\/td>Taq \/ AmpliTaq\u00ae<\/sup><\/td>Tfl<\/td>Stoffel Fragment<\/td>Tth<\/td>Vent\u00ae<\/sup> (exo-)<\/td>Vent\u00ae<\/sup> \/ (Tli)<\/td>Deep Vent\u2122<\/td>Pfu<\/td>Pwo<\/td>UlTma\u00ae<\/sup><\/td><\/tr>
5’\u21923′ Exo<\/td>\u3007<\/td>\u3007<\/td>X<\/td>\u3007<\/td>X<\/td>X<\/td>X<\/td>X<\/td>X<\/td>X<\/td><\/tr>
3’\u21925′ Exo<\/td>X<\/td>X<\/td>X<\/td>X<\/td>X<\/td>\u3007<\/td>\u3007<\/td>\u3007<\/td>\u3007<\/td>\u3007<\/td><\/tr>
RT<\/td>\u5f31<\/td>\u3007<\/td>\u5f31<\/td>\u3007<\/td>\u5f31<\/td>X<\/td>NA<\/td>NA<\/td>NA<\/td>\u5f31<\/td><\/tr>
\u5897\u5e45DNA \u672b\u7aef<\/td>3’A<\/td>3’A<\/td>3’A<\/td>3’A<\/td>70\uff05 \u5e73\u6ed1\u3000\u300030\uff05 1\u5869\u57fa\u7a81\u51fa<\/td>>95% \u5e73\u6ed1<\/td>\u5e73\u6ed1<\/td>\u5e73\u6ed1<\/td>\u5e73\u6ed1<\/td>>95% \u5e73\u6ed1<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

\u53c2\u8003\u6587\u732e\uff1a<\/strong><\/p>\n\n\n\n

  1. Newton, C.R. and Graham, A. (1994) PCR, BIOS Scientific Publishers Ltd., Oxford, UK.<\/li>
  2. Promega Connections https:\/\/www.promegaconnections.com\/a-quick-method-for-a-tailing-pcr-products\/<\/a><\/li><\/ol>\n","protected":false},"excerpt":{"rendered":"

    \u3042\u308b\u30a4\u30f3\u30b5\u30fc\u30c8\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306e\u52b9\u7387\u3092\u4e88\u6e2c\u3059\u308b\u3053\u3068\u306f\u4e0d\u53ef\u80fd\u3067\u3059\u3002\u305d\u306e\u52b9\u7387\u306f\u30a4\u30f3\u30b5\u30fc\u30c8\u306e\u30b5\u30a4\u30ba\u3001\u914d\u5217\u3001PCR\u7523\u7269\u306e\u7cbe\u88fd\u5ea6\u306a\u3069\u306e\u591a\u304f\u306e\u8981\u56e0\u306b\u4f9d\u5b58\u3059\u308b\u305f\u3081\u3067\u3059\u3002\u3053\u308c\u3089\u306e\u8981\u56e0\u306f\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u306e\u52b9\u7387\u3068\u4e26\u3073\u3001T-Vector\u3078\u306e\u30af\u30ed … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[123],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/443"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=443"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/443\/revisions"}],"predecessor-version":[{"id":1778,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/443\/revisions\/1778"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=443"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=443"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=443"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}