{"id":446,"date":"2020-12-02T15:28:25","date_gmt":"2020-12-02T06:28:25","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=446"},"modified":"2020-12-16T16:50:10","modified_gmt":"2020-12-16T07:50:10","slug":"q156","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/cloning\/cloning-2\/vector\/pgem-t-vector-system\/q156","title":{"rendered":"pGEM\u00ae-T Vector\u3001pGEM\u00ae-T Easy Vector\u3001pTARGET\u2122 Mammalian Expression Vector\u306e\u3088\u3046\u306aT-Vector\u3078\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306b\u9069\u3057\u305f\u8010\u71b1\u6027\u9175\u7d20\u306f\u3069\u308c\u3067\u3059\u304b\uff1f"},"content":{"rendered":"\n<p>3\u2019\u21925\u2019\u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027\u3092\u6b20\u3044\u305f\u8010\u71b1\u6027DNA Polymerase(\u4f8b\u3048\u3070<em>Taq<\/em>,&nbsp;<em>Tfl<\/em>,&nbsp;<em>Tth<\/em>&nbsp;DNA Polymerase)\u306fpGEM<sup>\u00ae<\/sup>-T Vector\u3084pGEM<sup>\u00ae<\/sup>-T Easy Vector\u306e\u3088\u3046\u306aT-vector\u306b\u9069\u7528\u3067\u304d\u307e\u3059\u3002<em>Tli<\/em>,&nbsp;<em>Pwo<\/em>,&nbsp;<em>Pfu<\/em>\u306e\u3088\u3046\u306a\u30d7\u30eb\u30fc\u30d5\u30ea\u30fc\u30c7\u30a3\u30f3\u30b0\u6a5f\u80fd\u3092\u3082\u3063\u305f\u8010\u71b1\u6027DNA Polymerase\u3067\u306f\u30013\u2019\u21925\u2019\u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027\u3092\u6709\u3057\u3066\u3044\u308b\u306e\u3067T-vector\u306b\u9069\u7528\u3067\u304d\u307e\u305b\u3093\u304c\u3001\u5897\u5e45\u5f8c\u306b<em>Taq<\/em>&nbsp;DNA Polymerase(\u307e\u305f\u306f\u985e\u4f3c\u306e\u9175\u7d20)\u3068dATP\u3092\u4f7f\u3063\u3066\u672b\u7aef\u306bA\u3092\u4ed8\u52a0\u3059\u308b\u3053\u3068\u3067\u9069\u7528\u3067\u304d\u307e\u3059(1)\u3002\u3053\u306e\u3088\u3046\u306b\u3001\u3059\u3079\u3066\u306ePCR\u7523\u7269\u306fT-vector\u306b\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u305f\u3060\u3057\u3001\u30d7\u30eb\u30fc\u30d5\u30ea\u30fc\u30c7\u30a3\u30f3\u30b0\u6a5f\u80fd\u3092\u6709\u3059\u308b\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3067\u306f\u4e0a\u8a18\u306e\u3088\u3046\u306a\u672b\u7aef\u4ed8\u52a0\u53cd\u5fdc\u304c\u5fc5\u8981\u3067\u3059\u3002\uff08PN67-FAQ)<\/p>\n\n\n\n<p><strong>\u53c2\u8003\u6587\u732e<\/strong>&nbsp;:<\/p>\n\n\n\n<p>1. pGEM<sup>\u00ae<\/sup>-T and pGEM<sup>\u00ae<\/sup>-T Easy Vector Systems Technical Manual #TM042, Promega Corporation.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>3\u2019\u21925\u2019\u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027\u3092\u6b20\u3044\u305f\u8010\u71b1\u6027DNA Polymerase(\u4f8b\u3048\u3070Taq,&nbsp;Tfl,&nbsp;Tth&nbsp;DNA Polymerase)\u306fpGEM\u00ae-T Vector\u3084pGEM\u00ae-T E &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[123],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/446"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=446"}],"version-history":[{"count":1,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/446\/revisions"}],"predecessor-version":[{"id":447,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/446\/revisions\/447"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=446"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=446"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=446"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}