{"id":458,"date":"2020-12-02T15:32:21","date_gmt":"2020-12-02T06:32:21","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=458"},"modified":"2020-12-21T11:39:20","modified_gmt":"2020-12-21T02:39:20","slug":"q161","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/cloning\/cloning-2\/system\/flexi-vector-system\/q161","title":{"rendered":"Flexi\u00ae Vectors\u306e\u5236\u9650\u9175\u7d20\u30b5\u30a4\u30c8\u4ed8\u8fd1\u306e\u914d\u5217\u3068Flexi\u00ae Vectors\u9593\u306e\u7d44\u307f\u63db\u3048\u3067\u306e\u6ce8\u610f\u70b9\u3092\u6559\u3048\u3066\u304f\u3060\u3055\u3044\u3002"},"content":{"rendered":"\n

FlexiR Vectors\u306f\u30011) \u30bf\u30b0\u306e\u878d\u5408\u306a\u3057 (pF1, pF3, pF4, pF5, pF9, pF12)\u30012) N\u672b\u7aef\u306b\u30bf\u30b0\u306e\u878d\u5408 (pFN2, pFN6, pFN10, pFN11)\u30013) C\u672b\u7aef\u306b\u30bf\u30b0\u306e\u878d\u5408 (pFC7, pFC8)\u3001\u306e3\u7a2e\u985e\u306b\u5206\u3051\u3089\u308c\u307e\u3059\u3002\u305d\u308c\u305e\u308c\u306e\u5236\u9650\u9175\u7d20\u30b5\u30a4\u30c8\u306e\u8a73\u7d30\u306a\u5869\u57fa\u914d\u5217\u60c5\u5831\u3092\u4ee5\u4e0b\u306b\u793a\u3057\u307e\u3059\u3002Sgf<\/em> I\u304a\u3088\u3073Pme<\/em> I\u3067\u5207\u308a\u51fa\u3059\u3053\u3068\u306b\u3088\u308a\u65b9\u5411\u6027\u3001\u8aad\u307f\u67a0\u3092\u7dad\u6301\u3057\u305f\u307e\u307e\u79fb\u3059\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002Flexi\u00ae<\/sup> Vector\u306bORF\u3092\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3059\u308b\u5834\u5408\u3001N\u672b\u7aef\u5074\u306fSgf I\u30b5\u30a4\u30c8\uff0bC\uff0binitiation codon ATG\u3068\u306a\u308b\u3088\u3046\u306b\u30c7\u30b6\u30a4\u30f3\u3057\u3001C\u672b\u7aef\u5074\u306fORF\uff0bPme<\/em> I\u3068\u3057\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306b\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001N\u672b\u7aef\u5074\u306b\u306fKozak\u30b3\u30f3\u30bb\u30f3\u30b5\u30b9\u914d\u5217\u304c\u5c0e\u5165\u3055\u308c\u3001C\u672b\u7aef\u5074\u306fPme<\/em> I\u30b5\u30a4\u30c8\u4e2d\u306eTAA\u304c\u76ee\u7684ORF\u306b\u5bfe\u3059\u308bstop codon\u3068\u3057\u3066\u5c0e\u5165\u3055\u308c\u307e\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u306b\u95a2\u3057\u307e\u3057\u3066\u306f\u3001\u300cQ.\u3000Flexi\u00ae<\/sup> Vector System\u306e\u4f7f\u7528\u65b9\u6cd5\u3092\u7c21\u5358\u306b\u8aac\u660e\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u300d\u3082\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002
<\/p>\n\n\n\n

\"\"
\u56f32\u3000C-terminal Flexi\u00ae<\/sup> Vector\u3078\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0<\/figcaption><\/figure>\n\n\n\n
  1. \u30bf\u30b0\u306e\u878d\u5408\u306a\u3057<\/strong>:RBS\u3001Kozak\u3001\u7d42\u6b62\u30b3\u30c9\u30f3\u304c\u542b\u307e\u308c\u307e\u3059\u3002
    <\/li>
  2. N\u672b\u306b\u30bf\u30b0\u306e\u878d\u5408<\/strong>:\u3000\u7d42\u6b62\u30b3\u30c9\u30f3\u304c\u542b\u307e\u308c\u307e\u3059\u3002
    <\/li>
  3. C\u672b\u306b\u30bf\u30b0\u306e\u878d\u5408:<\/strong> RBS\u3001Kozak\u3092\u542b\u307f\u307e\u3059\u3002
    \u76ee\u7684\u907a\u4f1d\u5b50\u5c0e\u5165\u5f8c\u306f\u3001Pme<\/em> I\u3068Eco<\/em>ICRI\u306e\u4e21\u65b9\u306e\u30b5\u30a4\u30c8\u304c\u5931\u308f\u308c\u307e\u3059\u3002
    <\/li><\/ol>\n\n\n\n

    \u30d9\u30af\u30bf\u30fc\u540d\u306b\u300cC\u300d\u3092\u542b\u3080C-terminal Flexi\u00ae<\/sup> Vector(pFC7, pFC8)\u306f\u3001\u7d42\u6b62\u30b3\u30c9\u30f3\u304c\u5c0e\u5165\u3055\u308c\u308bPme<\/em> I\u306e\u4ee3\u308f\u308a\u306b\u7d42\u6b62\u30b3\u30c9\u30f3\u3092\u542b\u307e\u306a\u3044Eco<\/em>ICR I\u3092\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u3002C-terminal Flexi\u00ae<\/sup> Vector\u306b\u76ee\u7684\u907a\u4f1d\u5b50\u3092\u5c0e\u5165\u3059\u308b\u3068\u3001Pme<\/em> I\u3068Eco<\/em>ICRI\u306e\u4e21\u65b9\u306e\u30b5\u30a4\u30c8\u304c\u5931\u308f\u308c\u307e\u3059\u306e\u3067Pme I<\/em>\u30b5\u30a4\u30c8\u4e2d\u306eStop codon\u306f\u306a\u304f\u306a\u308a\u307e\u3059\u304c\u3001\u5236\u9650\u9175\u7d20\u3067\u518d\u5207\u65ad\u3067\u304d\u307e\u305b\u3093\u306e\u3067\u3001\u4ed6\u306eFlexi\u00ae<\/sup> Vectors\u306b\u7d44\u307f\u63db\u3048\u308b\u3053\u3068\u304c\u51fa\u6765\u306a\u304f\u306a\u308a\u307e\u3059\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

    FlexiR Vectors\u306f\u30011) \u30bf\u30b0\u306e\u878d\u5408\u306a\u3057 (pF1, pF3, pF4, pF5, pF9, pF12)\u30012) N\u672b\u7aef\u306b\u30bf\u30b0\u306e\u878d\u5408 (pFN2, pFN6, pFN10, pFN11)\u30013) C\u672b\u7aef\u306b\u30bf\u30b0\u306e\u878d … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[124],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/458"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=458"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/458\/revisions"}],"predecessor-version":[{"id":1788,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/458\/revisions\/1788"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=458"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=458"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=458"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}