{"id":460,"date":"2020-12-02T15:33:25","date_gmt":"2020-12-02T06:33:25","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=460"},"modified":"2020-12-21T12:06:28","modified_gmt":"2020-12-21T03:06:28","slug":"q162","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/cloning\/cloning-2\/system\/flexi-vector-system\/q162","title":{"rendered":"Flexi\u00ae Vector System\u306f\u57fa\u672c\u7684\u306bSgf I\u304a\u3088\u3073Pme I\u3092\u5229\u7528\u3057\u305fdirectional cloning system\u306e\u3088\u3046\u3067\u3059\u304c\u3001Sgf I\u304a\u3088\u3073EcoICRI\u30b5\u30a4\u30c8\u3092\u5229\u7528\u3059\u308bC-terminal Flexi\u00ae Vector\u3067Entry clone\u3092\u4f5c\u88fd\u3057\u3066\u3082\u3044\u3044\u3067\u3059\u304b\uff1f"},"content":{"rendered":"\n<p>C\u672b\u7aef\u30bf\u30b0\u30b3\u30f3\u30b9\u30c8\u30e9\u30af\u30c8\u306e\u307f\u5fc5\u8981\u3067\u3001\u5c06\u6765\u7684\u306b\u3082N\u672b\u7aef\u30bf\u30b0\u3001\u3042\u308b\u3044\u306f\u30bf\u30b0\u306a\u3057\u306eFlexi<sup>\u00ae<\/sup>\u00a0Vector\u306b\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3059\u308b\u3053\u3068\u306f\u306a\u3044\u3068\u3044\u3046\u3053\u3068\u3067\u3057\u305f\u3089\u3001C-terminal Flexi<sup>\u00ae<\/sup>\u00a0Vector\u3067Entry clone\u3092\u4f5c\u88fd\u3057\u3066\u3082\u3044\u3044\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u305f\u3060\u3057\u3001<em>Pme<\/em>\u00a0I\u304a\u3088\u3073EcoICRI\u30b5\u30a4\u30c8\u3092\u7528\u3044\u305fC-terminal Flexi<sup>\u00ae<\/sup>\u00a0Vector\u306f\u3001<em>Pme<\/em>\u00a0I\u30b5\u30a4\u30c8\u306e\u3088\u3046\u306bstop codon\u3092\u5c0e\u5165\u3057\u306a\u3044\u76ee\u7684\u3067EcoICRI\u30b5\u30a4\u30c8\u3092\u63a1\u7528\u3057\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001<em>Pme<\/em>\u00a0I\uff0dEcoICRI\u65ad\u7247\u3092N\u672b\u7aef\u30bf\u30b0\u3001\u3042\u308b\u3044\u306f\u30bf\u30b0\u306a\u3057\u306eFlexi<sup>\u00ae<\/sup>\u00a0Vector\u306b\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3059\u308b\u3068stop codon\u304c\u5c0e\u5165\u3055\u308c\u307e\u305b\u3093\u306e\u3067\u3001\u672c\u6765\u306eORF\u3067\u7ffb\u8a33\u3092stop\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u305b\u3093\u3002\u5f93\u3044\u307e\u3057\u3066\u30d7\u30ed\u30e1\u30ac\u3067\u306f\u3001\u30de\u30cb\u30e5\u30a2\u30eb\u3001\u304a\u3088\u3073Flexi<sup>\u00ae<\/sup>\u00a0Vector Primer Design Tool\u3067\u306f\u3001\u300cEntry\u300d\u306e\u65b9\u6cd5\u3068\u3057\u3066<em>Sgf<\/em>\u00a0I\uff0d<em>Pme<\/em>\u00a0I\u3092\u5229\u7528\u3057\u305fFlexi<sup>\u00ae<\/sup>\u00a0Vector\u3078\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u65b9\u6cd5\u306e\u307f\u3054\u7d39\u4ecb\u3057\u3066\u304a\u308a\u307e\u3059\u3002\u300c<a href=\"https:\/\/www.promega.co.jp\/resources\/faq\/cloning\/cloning-2\/system\/flexi-vector-system\/q157\" target=\"_blank\" rel=\"noreferrer noopener\">\u3053\u3061\u3089<\/a>\u300d\u306b\u7c21\u5358\u306a\u8aac\u660e\u56f3\u304c\u3054\u3056\u3044\u307e\u3059\u306e\u3067\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>C\u672b\u7aef\u30bf\u30b0\u30b3\u30f3\u30b9\u30c8\u30e9\u30af\u30c8\u306e\u307f\u5fc5\u8981\u3067\u3001\u5c06\u6765\u7684\u306b\u3082N\u672b\u7aef\u30bf\u30b0\u3001\u3042\u308b\u3044\u306f\u30bf\u30b0\u306a\u3057\u306eFlexi\u00ae\u00a0Vector\u306b\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3059\u308b\u3053\u3068\u306f\u306a\u3044\u3068\u3044\u3046\u3053\u3068\u3067\u3057\u305f\u3089\u3001C-terminal Flexi\u00ae\u00a0Vector\u3067Entry clon &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[124],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/460"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=460"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/460\/revisions"}],"predecessor-version":[{"id":1789,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/460\/revisions\/1789"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=460"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=460"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=460"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}