{"id":462,"date":"2020-12-02T15:34:32","date_gmt":"2020-12-02T06:34:32","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=462"},"modified":"2020-12-22T13:26:33","modified_gmt":"2020-12-22T04:26:33","slug":"q163","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/cloning\/cloning-2\/system\/flexi-vector-system\/q163","title":{"rendered":"Flexi\u00ae Vector System\u306b\u304a\u3044\u3066\u3001\u30d9\u30af\u30bf\u30fc\u4ee5\u5916\u306b\u5fc5\u8981\u306a\u8a66\u85ac\u3092\u6559\u3048\u3066\u304f\u3060\u3055\u3044\u3002"},"content":{"rendered":"\n

\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u8a66\u85ac\u304c\u5fc5\u8981\u3067\u3059\u3002\u30d7\u30ed\u30e1\u30ac\u3067\u306f\u3001\u521d\u3081\u3066Flexi\u00ae<\/sup> Vector\u306b\u76ee\u7684\u907a\u4f1d\u5b50\u3092\u5c0e\u5165\u3059\u308b\u5834\u5408\uff08\u30a8\u30f3\u30c8\u30ea\u30fc\uff1aEntry\uff09\u3068\u76ee\u7684\u907a\u4f1d\u5b50\u3092\u4ed6\u306eFlexi\u00ae<\/sup> Vector\u306b\u7d44\u307f\u63db\u3048\u308b\u5834\u5408\uff08\u30c8\u30e9\u30f3\u30b9\u30d5\u30a1\u30fc\uff1aTransfer\uff09\u306b\u5fc5\u8981\u306a\u8a66\u85ac\u3092\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002Flexi\u00ae<\/sup> System, Entry\/Transfer(C8640)\u306b\u306f\u3001\u30a4\u30f3\u30b5\u30fc\u30c8\u306e\u7cbe\u88fd\u306b\u5fc5\u8981\u306aWizard\u00ae<\/sup> SV Gel and PCR Clean-Up System(A9280)\u3082\u542b\u307e\u308c\u307e\u3059\u3002\u8a66\u85ac\u306e\u69cb\u6210\u306b\u95a2\u3057\u307e\u3057\u3066\u306f\u3001\u88683\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\u88683\u3000\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u8a66\u85ac\u306e\u69cb\u6210\u54c1<\/p>\n\n\n\n

\u8a66\u85ac<\/th>\u30b5\u30a4\u30ba<\/th>\u30ab\u30bf\u30ed\u30b0\u756a\u53f7<\/th>\u4fa1\u683c(\u00a5)<\/th><\/tr>
Flexi\u00ae<\/sup> System, Entry\/Transfer
\uff08\u30d9\u30af\u30bf\u30fc\u3078\u306e\u5c0e\u5165\u53cd\u5fdc5\u56de\u5206
\u3000\u304a\u3088\u3073\u79fb\u52d5\u53cd\u5fdc20\u56de\u5206\uff09<\/td>		5\/20\u56de\u5206<\/td>C8640<\/td>30,000<\/td><\/tr>
2 \u00d7 50\u00b5l<\/td>Flexi\u00ae<\/sup> Enzyme Blend
(Sgf<\/em> I and Pme<\/em> I)<\/td><\/tr>
500u<\/td>T4 DNA Ligase (HC)<\/td><\/tr>
1ml<\/td>5X Flexi\u00ae<\/sup> Digest Buffer<\/td><\/tr>
500\u00b5l<\/td>2X Flexi\u00ae<\/sup> Ligase Buffer<\/td><\/tr>
1.25ml<\/td>Nuclease-Free Water<\/td><\/tr>
Cat.# A9280 WizardR SV Gel and PCR Clean-Up System<\/td><\/tr>
\u8a66\u85ac<\/th>\u30b5\u30a4\u30ba<\/th>\u30ab\u30bf\u30ed\u30b0\u756a\u53f7<\/th>\u4fa1\u683c(\u00a5)<\/th><\/tr>
Flexi System, Transfer
\uff08\u30d9\u30af\u30bf\u30fc\u9593\u306e\u79fb\u52d5\u53cd\u5fdc100\u56de\u5206\uff09<\/td>		100\u56de\u5206<\/td>C8820<\/td>98,000<\/td><\/tr>
4 \u00d7 50\u00b5l<\/td>Flexi\u00ae<\/sup> Enzyme Blend
(Sgf<\/em> I and Pme<\/em> I)<\/td><\/tr>
4 \u00d7 500u<\/td>T4 DNA Ligase (HC)<\/td><\/tr>
1ml<\/td>5X Flexi\u00ae<\/sup> Digest Buffer<\/td><\/tr>
2 \u00d7 500\u00b5l<\/td>2X Flexi\u00ae<\/sup> Ligase Buffer<\/td><\/tr>
2 \u00d7 1.25ml<\/td>Nuclease-Free Water<\/td><\/tr>
\u8a66\u85ac<\/th>\u30b5\u30a4\u30ba<\/th>\u30ab\u30bf\u30ed\u30b0\u756a\u53f7<\/th>\u4fa1\u683c(\u00a5)<\/th><\/tr>
Carboxy Flexi\u00ae<\/sup> System, Transfer
\uff08C\u672b\u7aef\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u7528
\u3000\u30d9\u30af\u30bf\u30fc\u9593\u306e\u79fb\u52d5\u53cd\u5fdc100\u56de\u5206\uff09<\/td>		50\u56de\u5206<\/td>C9320<\/td>55,000<\/td><\/tr>
50\u00b5l<\/td>Carboxy Flexi\u00ae<\/sup> Enzyme Blend
(Sgf<\/em> I and Eco<\/em>ICR I)<\/td><\/tr>
50\u00b5l<\/td>Flexi\u00ae<\/sup> Enzyme Blend
(Sgf<\/em> I and Pme<\/em> I)<\/td><\/tr>
2 \u00d7 500u<\/td>T4 DNA Ligase (HC)<\/td><\/tr>
1ml<\/td>5X Flexi\u00ae<\/sup> Digest Buffer<\/td><\/tr>
500\u00b5l<\/td>2X Flexi\u00ae<\/sup> Ligase Buffer<\/td><\/tr>
1.25ml<\/td>Nuclease-Free Water<\/td><\/tr><\/tbody><\/table><\/figure>\n","protected":false},"excerpt":{"rendered":"

\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u8a66\u85ac\u304c\u5fc5\u8981\u3067\u3059\u3002\u30d7\u30ed\u30e1\u30ac\u3067\u306f\u3001\u521d\u3081\u3066Flexi\u00ae Vector\u306b\u76ee\u7684\u907a\u4f1d\u5b50\u3092\u5c0e\u5165\u3059\u308b\u5834\u5408\uff08\u30a8\u30f3\u30c8\u30ea\u30fc\uff1aEntry\uff09\u3068\u76ee\u7684\u907a\u4f1d\u5b50\u3092\u4ed6\u306eFlexi\u00ae Vector\u306b\u7d44\u307f\u63db\u3048\u308b\u5834\u5408\uff08\u30c8\u30e9\u30f3\u30b9\u30d5\u30a1 … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[124],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/462"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=462"}],"version-history":[{"count":11,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/462\/revisions"}],"predecessor-version":[{"id":1871,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/462\/revisions\/1871"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=462"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=462"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=462"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}