{"id":468,"date":"2020-12-02T15:36:13","date_gmt":"2020-12-02T06:36:13","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=468"},"modified":"2020-12-21T12:09:09","modified_gmt":"2020-12-21T03:09:09","slug":"q166","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/cloning\/cloning-2\/system\/flexi-vector-system\/q166","title":{"rendered":"Flexi\u00ae Vector System\u306e\u4f7f\u7528\u65b9\u6cd5\u3092\u7c21\u5358\u306b\u8aac\u660e\u3057\u3066\u304f\u3060\u3055\u3044\u3002"},"content":{"rendered":"\n<p>\u76ee\u7684\u907a\u4f1d\u5b50\u3092\u521d\u3081\u3066Flexi<sup>\u00ae<\/sup>&nbsp;Vector\u306b\u5c0e\u5165\u3059\u308b\u5834\u5408\uff08\u30a8\u30f3\u30c8\u30ea\u30fc\uff09\u3068\u76ee\u7684\u907a\u4f1d\u5b50\u3092\u4ed6\u306eFlexi<sup>\u00ae<\/sup>&nbsp;Vector\u306b\u7d44\u307f\u63db\u3048\u308b\u5834\u5408\uff08\u30c8\u30e9\u30f3\u30b9\u30d5\u30a1\u30fc\uff09\u306b\u3064\u3044\u3066\u8aac\u660e\u3057\u307e\u3059\u3002\u8a73\u7d30\u306f<a href=\"http:\/\/www.promega.com\/tbs\/tm254\/tm254.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">\u88fd\u54c1\u30d7\u30ed\u30c8\u30b3\u30fc\u30ebTM254<\/a>\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<p><strong>1) \u76ee\u7684\u907a\u4f1d\u5b50\u3092\u521d\u3081\u3066Flexi<sup>\u00ae<\/sup>&nbsp;Vector<\/strong><strong>\u306b\u5c0e\u5165\u3059\u308b\uff08\u30a8\u30f3\u30c8\u30ea\u30fc\uff09<\/strong><\/p>\n\n\n\n<p><em>Sgf&nbsp;<\/em>I\u3068&nbsp;<em>Pme<\/em>&nbsp;I\u3092\u542b\u3080\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u7528\u3044\u3066\u76ee\u7684\u907a\u4f1d\u5b50\u3092PCR\u3067\u5897\u5e45\u3057\u3001\u5b9f\u9a13\u306b\u9069\u3057\u305fFlexi<sup>\u00ae<\/sup>&nbsp;Vector\u306b\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<p>&lt;\u30b9\u30c6\u30c3\u30d71\uff1b\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u8a2d\u8a08\u3068PCR><br><a rel=\"noreferrer noopener\" href=\"http:\/\/www.promega.com\/resources\/tools\/flexi-vector-primer-design-tool\/\" target=\"_blank\">Flexi<sup>\u00ae<\/sup>\u00a0Vector Primer Design Tool<\/a>\u3092\u7528\u3044\u3066\u3001<em>Sgf<\/em>\u00a0I\u304a\u3088\u3073<em>Pme<\/em>I\u30b5\u30a4\u30c8\u3092\u542b\u3080\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u8a2d\u8a08\u3057\u307e\u3059\u3002\u3053\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u7528\u3044\u3066PCR\u3092\u884c\u3044\u3001\u76ee\u7684\u907a\u4f1d\u5b50\u3092\u5897\u5e45\u3057\u307e\u3059(\u56f33)\u3002<br><\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"580\" height=\"330\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/pic_flexivector_04.gif\" alt=\"\" class=\"wp-image-1790\"\/><figcaption>\u56f33\u3000\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u3068PCR<\/figcaption><\/figure>\n\n\n\n<p>&lt;\u30b9\u30c6\u30c3\u30d72\uff1b\u30af\u30ed\u30fc\u30cb\u30f3\u30b0&gt;<br>PCR\u7523\u7269\u306f\u3001Wizard<sup>\u00ae<\/sup>&nbsp;SV Gel and PCR Clean-Up System \u3067\u7cbe\u88fd\u3057\u307e\u3059\u3002\u6b21\u306b\u3001Flexi<sup>\u00ae<\/sup>&nbsp;Enzyme Blend (<em>Sgf<\/em>&nbsp;I and&nbsp;<em>Pme<\/em>&nbsp;I)\u3092\u7528\u3044\u3066\u3001PCR\u7523\u7269\u3068Flexi<sup>\u00ae<\/sup>&nbsp;Vector\u3092\u305d\u308c\u305e\u308c\u5207\u65ad\u3057\u3001PCR\u7523\u7269\u3092Wizard<sup>\u00ae<\/sup>&nbsp;SV Gel and PCR Clean-Up System \u3067\u7cbe\u88fd\u3057\u307e\u3059*\uff09\u3002\u305d\u306e\u5f8c\u3001\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a9\u30fc\u30e1\u30fc\u30b7\u30e7\u30f3\u3092\u884c\u3044\u307e\u3059\u3002\u30d9\u30af\u30bf\u30fc\u306b\u542b\u307e\u308c\u308b\u81f4\u6b7b\u907a\u4f1d\u5b50(lethal gene\uff1bBarnase)\u3092\u3082\u3064\u30af\u30ed\u30fc\u30f3\u306f\u751f\u80b2\u3067\u304d\u306a\u3044\u305f\u3081\u3001\u76ee\u7684\u907a\u4f1d\u5b50\u3092\u542b\u3080\u30af\u30ed\u30fc\u30f3\u306e\u307f\u304c\u5f97\u3089\u308c\u307e\u3059\u3002\u307e\u305f\u3001\u57f9\u5730\u306b\u306f\u3001\u5fc5\u305a\u30a2\u30f3\u30d4\u30b7\u30ea\u30f3\u3082\u3057\u304f\u306f\u30ab\u30ca\u30de\u30a4\u30b7\u30f3\u3092\u6dfb\u52a0\u3057\u3001\u85ac\u5264\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3082\u5b9f\u65bd\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<p>*\uff09\u4e00\u822c\u7684\u306a\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3067\u306f\u3001\u5236\u9650\u9175\u7d20\u51e6\u7406\u3057\u305f\u30d9\u30af\u30bf\u30fc\u304b\u3089\u5207\u308a\u51fa\u3055\u308c\u305fDNA\u65ad\u7247\u3092\u9664\u304f\u305f\u3081\u306b\u30d9\u30af\u30bf\u30fc\u306e\u7cbe\u88fd\u3092\u884c\u3046\u5834\u5408\u3082\u3042\u308a\u307e\u3059\u304c\u3001Flexi<sup>\u00ae<\/sup>&nbsp;Vector\u3067\u306f\u3001<em>Sgf<\/em>&nbsp;I \u3068<em>Pme<\/em>&nbsp;I\u306e\u51e6\u7406\u3067\u81f4\u6b7b\u907a\u4f1d\u5b50\u304c\u5207\u308a\u51fa\u3055\u308c\u3001\u305d\u308c\u3092\u542b\u3080\u30af\u30ed\u30fc\u30f3\u306f\u751f\u80b2\u3067\u304d\u306a\u3044\u305f\u3081\u3001\u30d9\u30af\u30bf\u30fc\u306e\u7cbe\u88fd\u306f\u4e0d\u8981\u3067\u3059 \u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"420\" height=\"610\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/pic_flexivector_05.gif\" alt=\"\" class=\"wp-image-1792\"\/><figcaption>\u56f34\u3000Flexi<sup>\u00ae<\/sup>\u00a0Vector System\u3000\u30a8\u30f3\u30c8\u30ea\u30fc\u306e\u6982\u7565<\/figcaption><\/figure>\n\n\n\n<p><strong>2) \u76ee\u7684\u907a\u4f1d\u5b50\u3092\u4ed6\u306e<\/strong><strong>Flexi<sup>\u00ae<\/sup>&nbsp;Vector<\/strong><strong>\u306b\u7d44\u307f\u63db\u3048\u308b\uff08\u30c8\u30e9\u30f3\u30b9\u30d5\u30a1\u30fc\uff09<\/strong><\/p>\n\n\n\n<p>\u76ee\u7684\u907a\u4f1d\u5b50\u3092\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3057\u305f\u30d9\u30af\u30bf\u30fc\uff08Donor\uff09\u304b\u3089\u4ed6\u306e\u6a5f\u80fd\u3092\u6709\u3059\u308bFlexi<sup>\u00ae<\/sup>&nbsp;Vector\uff08Acceptor\uff09\u306b\u7d44\u307f\u63db\u3048\u308b\u5834\u5408*\uff09\u3001\u3053\u308c\u30892\u7a2e\u985e\u306e\u30d9\u30af\u30bf\u30fc\u3092\u540c\u6642\u306bFlexi<sup>\u00ae<\/sup>&nbsp;Enzyme Blend (<em>Sgf<\/em>&nbsp;I and&nbsp;<em>Pme<\/em>&nbsp;I)\u3067\u5207\u65ad\u3057\u307e\u3059\u3002\u5f15\u304d\u7d9a\u304d\u3001\u7cbe\u88fd\u64cd\u4f5c\u3092\u884c\u3046\u3053\u3068\u306a\u304f\u3001\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a9\u30fc\u30e1\u30fc\u30b7\u30e7\u30f3\u3092\u884c\u3044\u307e\u3059\u3002Acceptor\u3068\u306a\u308b\u30d9\u30af\u30bf\u30fc\u304c\u3082\u3064\u85ac\u5264\u8010\u6027(Amp<sup>r<\/sup>\u3042\u308b\u3044\u306fKan<sup>r<\/sup>)\u3068\u81f4\u6b7b\u907a\u4f1d\u5b50(lethal gene)\u306b\u3088\u308b\u9078\u629e\u306b\u3088\u308a\u3001\u76ee\u7684\u30af\u30ed\u30fc\u30f3\u3092\u9ad8\u52b9\u7387\u306b\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059(\u56f35)\u3002<\/p>\n\n\n\n<p>* \uff09Acceptor \u306b\u306fDonor\u3068\u7570\u306a\u308b\u85ac\u5264\u8010\u6027\u30de\u30fc\u30ab\u30fc\u3092\u3082\u3064\u30d9\u30af\u30bf\u30fc\u3092\u9078\u629e\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"580\" height=\"310\" src=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/pic_flexivector_06.gif\" alt=\"\" class=\"wp-image-1793\"\/><figcaption>\u56f35\u3000Flexi<sup>\u00ae<\/sup>\u00a0Vector System\u3000\u30c8\u30e9\u30f3\u30b9\u30d5\u30a1\u30fc\u306e\u6982\u7565<\/figcaption><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>\u76ee\u7684\u907a\u4f1d\u5b50\u3092\u521d\u3081\u3066Flexi\u00ae&nbsp;Vector\u306b\u5c0e\u5165\u3059\u308b\u5834\u5408\uff08\u30a8\u30f3\u30c8\u30ea\u30fc\uff09\u3068\u76ee\u7684\u907a\u4f1d\u5b50\u3092\u4ed6\u306eFlexi\u00ae&nbsp;Vector\u306b\u7d44\u307f\u63db\u3048\u308b\u5834\u5408\uff08\u30c8\u30e9\u30f3\u30b9\u30d5\u30a1\u30fc\uff09\u306b\u3064\u3044\u3066\u8aac\u660e\u3057\u307e\u3059\u3002\u8a73\u7d30\u306f\u88fd\u54c1\u30d7\u30ed\u30c8\u30b3\u30fc\u30ebTM25 &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[124],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/468"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=468"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/468\/revisions"}],"predecessor-version":[{"id":1794,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/468\/revisions\/1794"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=468"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=468"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=468"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}