{"id":524,"date":"2020-12-02T16:12:09","date_gmt":"2020-12-02T07:12:09","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=524"},"modified":"2021-02-15T17:58:19","modified_gmt":"2021-02-15T08:58:19","slug":"q188","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/cloning\/enzyme\/restriction-enzyme\/re-enzyme-info\/q188","title":{"rendered":"2\u7a2e\u985e\u4ee5\u4e0a\u306e\u5236\u9650\u9175\u7d20\u51e6\u7406\u3092\u540c\u6642\u306b\u884c\u3046\u306b\u306f\u3069\u306e\u3088\u3046\u306b\u3059\u308c\u3070\u826f\u3044\u3067\u3057\u3087\u3046\u304b\u3002"},"content":{"rendered":"\n

\u3059\u3079\u3066\u306e\u9175\u7d20\u306e\u6700\u9069\u306a\u53cd\u5fdc\u30d0\u30c3\u30d5\u30a1\u30fc\u304c\u540c\u4e00\u3067\u3042\u308b\u5834\u5408\u3001\u305d\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u3067\u884c\u3048\u307e\u3059\u3002
\u9175\u7d20\u91cf\u306e\u5408\u8a08\u304c\u53cd\u5fdc\u6db2\u91cf\u306e10%\u3092\u8d85\u3048\u306a\u3044\u3088\u3046\u306b\u52a0\u3048\u307e\u3059\u3002<\/p>\n\n\n\n

\u4f8b\uff1a<\/p>\n\n\n\n

\u6ec5\u83cc\u6e08\u307fNuclease-Free Water<\/td>13\u03bcl<\/td><\/tr>
\u5236\u9650\u9175\u7d20\u53cd\u5fdc\u30d0\u30c3\u30d5\u30a1\u30fcE<\/td>2\u03bcl<\/td><\/tr>
\u30a2\u30bb\u30c1\u30eb\u5316BSA(1mg\/ml) *<\/td>2\u03bcl<\/td><\/tr>
DNA sample (0.2~1\u03bcg) in D.W.\u307e\u305f\u306fTE\u30d0\u30c3\u30d5\u30a1\u30fc<\/td>1\u03bcl<\/td><\/tr>
BamHI (10u\/\u03bcl)<\/td>1\u03bcl<\/td><\/tr>
HindIII (10u\/\u03bcl)<\/td>1\u03bcl<\/td><\/tr>
\u6700\u7d42\u53cd\u5fdc\u91cf<\/td>20\u03bcl<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

*\u30d7\u30ed\u30e1\u30ac\u306e\u5236\u9650\u9175\u7d20\u306b\u306f10mg\/ml\u306e\u30a2\u30bb\u30c1\u30eb\u5316BSA\u304c\u6dfb\u4ed8\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u3092\u4f7f\u7528\u524d\u306bNuclease-Free Water\u306710\u500d\u5e0c\u91c8\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u3082\u3057\u304f\u306f\u300110mg\/ml\u306e\u30a2\u30bb\u30c1\u30eb\u5316BSA(\u539f\u6db2)\u3092100\u500d\u5e0c\u91c8\u306b\u306a\u308b\u3088\u3046\u306b\u5236\u9650\u9175\u7d20\u53cd\u5fdc\u6db2\u306b\u76f4\u63a5\u52a0\u3048\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\u540c\u6642\u306b\u51e6\u7406\u3057\u305f\u3044\u9175\u7d20\u304c\u540c\u4e00\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u3067\u6700\u9069\u306a\u6d3b\u6027\u3092\u793a\u3055\u306a\u3044\u5834\u5408\u306b\u306f\u3001\u4ee5\u4e0b\u306e\u3088\u3046\u306a\u65b9\u6cd5\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n

  1. \u30d0\u30c3\u30d5\u30a1\u30fc\u4e92\u63db\u6027\u306e\u8868\u3092\u53c2\u8003\u306b\u3057\u307e\u3059\u3002\u305d\u308c\u305e\u308c\u306e\u9175\u7d20\u306e\u6700\u9069\u306a\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u304a\u3051\u308b\u3082\u3046\u4e00\u65b9\u306e\u9175\u7d20\u306e\u6d3b\u6027\u3092\u6bd4\u8f03\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u307b\u3068\u3093\u3069\u306e\u5834\u5408\u3001\u30d7\u30ed\u30e1\u30ac\u306e4-CORER 10\u00d7 Buffer\u306e\u3044\u305a\u308c\u304b\u3001\u307e\u305f\u306fMULTI-CORE\u2122 Buffer\u3067\u826f\u597d\u306a\u6d3b\u6027\u304c\u793a\u3055\u308c\u308b\u3067\u3057\u3087\u3046\u3002\u3054\u5229\u7528\u306b\u306a\u308b\u30d0\u30c3\u30d5\u30a1\u30fc\u3067\u6d3b\u6027\u304c75%\u4ee5\u4e0b\u306e\u5834\u5408\u306f\u3001\u53cd\u5fdc\u6642\u9593\u3092\u5ef6\u9577\u3059\u308b\u304b\u3001\u52a0\u3048\u308b\u9175\u7d20\u306e\u30e6\u30cb\u30c3\u30c8\u6570\u3092\u5897\u3084\u3057\u305f\u307b\u3046\u304c\u826f\u3044\u3067\u3057\u3087\u3046\u3002\u3053\u306e\u5834\u5408\u306b\u306fStar\u6d3b\u6027\u306b\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044\u3002<\/li>
  2. \u3088\u308a\u4f7f\u3044\u3084\u3059\u3044\u30d0\u30c3\u30d5\u30a1\u30fc\u4e92\u63db\u6027\u3092\u793a\u3059\u30a4\u30bd\u30b7\u30be\u30de\u30fc\u3084\u30cd\u30aa\u30b7\u30be\u30de\u30fc\u3092\u9078\u629e\u3057\u307e\u3059\u3002\u5236\u9650\u9175\u7d20\u306e\u7279\u6027\u306b\u8a18\u8f09\u3055\u308c\u3066\u3044\u308b\u5236\u9650\u9175\u7d20\u95a2\u9023\u60c5\u5831 (https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/cat18_2012.pdf<\/a> page 18.20-21 )\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/li>
  3. \u4f4e\u5869\u6fc3\u5ea6\u30d0\u30c3\u30d5\u30a1\u30fc\u3067\u6700\u9069\u306a\u6d3b\u6027\u3092\u793a\u3059\u9175\u7d20\u3092\u7528\u3044\u3066\u6700\u521d\u306e\u6d88\u5316\u3092\u884c\u3044\u307e\u3059\u3002\u3053\u306e\u53cd\u5fdc\u306b\u7d9a\u3051\u306665\u2103\u300110\u5206\u9593\u306e\u52a0\u71b1\u306b\u3088\u308b\u9175\u7d20\u306e\u4e0d\u6d3b\u6027\u5316\u3092\u884c\u3044\u307e\u3059\u3002\u4e00\u90e8\u306e\u9175\u7d20\u3067\u306f\u71b1\u306b\u5bfe\u3057\u3066\u9ad8\u3044\u5b89\u5b9a\u6027\u3092\u793a\u3059\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u306e\u3067\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044\u3002\u3053\u306e\u53cd\u5fdc\u6db2\u306b\u30012\u7a2e\u985e\u76ee\u306e\u9175\u7d20\u306e10\u00d7\u53cd\u5fdc\u30d0\u30c3\u30d5\u30a1\u30fc\u3001BSA(1mg\/ml)\u30012\u7a2e\u985e\u76ee\u306e\u9175\u7d20\u3001\u9069\u5f53\u91cf\u306eD.W.\u3092\u52a0\u3048\u3001\u7dcf\u91cf\u3067\u6700\u521d\u306e\u53cd\u5fdc\u6db2\u306e5\u500d\u91cf\u306b\u306a\u308b\u3088\u3046\u306b\u3057\u3001\u9069\u6e29\u3067\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3057\u307e\u3059\u3002<\/li>
  4. \u305d\u308c\u305e\u308c\u306e\u6d88\u5316\u3092\u6700\u9069\u306a\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u4f7f\u3063\u3066\u9023\u7d9a\u3057\u3066\u884c\u3044\u307e\u3059\u3002\u6700\u521d\u306e\u6d88\u5316\u306e\u7d42\u4e86\u5f8c\u3001Wizard\u00ae<\/sup> DNA Clean-Up System(\u30ab\u30bf\u30ed\u30b0\u756a\u53f7 A7280)\u3092\u7528\u3044\u305f\u7cbe\u88fd\u307e\u305f\u306f\u52a0\u71b1\u306b\u3088\u308b\u9175\u7d20\u306e\u4e0d\u6d3b\u6027\u5316\u3092\u884c\u3044\u3001\u30a8\u30bf\u30ce\u30fc\u30eb\u6c88\u6bbf\u3092\u884c\u3044\u307e\u3059\u3002\u305d\u3057\u3066\u30012\u7a2e\u985e\u76ee\u306e\u9175\u7d20\u306b\u3088\u308b\u6d88\u5316\u3092\u884c\u3044\u307e\u3059\u3002<\/li><\/ol>\n\n\n\n

    (eNotes-FAQ002)<\/p>\n","protected":false},"excerpt":{"rendered":"

    \u3059\u3079\u3066\u306e\u9175\u7d20\u306e\u6700\u9069\u306a\u53cd\u5fdc\u30d0\u30c3\u30d5\u30a1\u30fc\u304c\u540c\u4e00\u3067\u3042\u308b\u5834\u5408\u3001\u305d\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u3067\u884c\u3048\u307e\u3059\u3002\u9175\u7d20\u91cf\u306e\u5408\u8a08\u304c\u53cd\u5fdc\u6db2\u91cf\u306e10%\u3092\u8d85\u3048\u306a\u3044\u3088\u3046\u306b\u52a0\u3048\u307e\u3059\u3002 \u4f8b\uff1a \u6ec5\u83cc\u6e08\u307fNuclease-Free Water 13\u03bcl \u5236\u9650\u9175\u7d20\u53cd\u5fdc\u30d0\u30c3\u30d5\u30a1 … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[126],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/524"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=524"}],"version-history":[{"count":4,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/524\/revisions"}],"predecessor-version":[{"id":2466,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/524\/revisions\/2466"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=524"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=524"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=524"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}