{"id":532,"date":"2020-12-02T16:14:49","date_gmt":"2020-12-02T07:14:49","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=532"},"modified":"2020-12-16T17:15:47","modified_gmt":"2020-12-16T08:15:47","slug":"q191","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/cloning\/enzyme\/dnase\/rnase-free-dnase\/q191","title":{"rendered":"RQ1 RNase-Free DNase\u3092\u3069\u306e\u3088\u3046\u306b\u4f7f\u3063\u305f\u3089\u3001RT-PCR\u306b\u4f7f\u3046\u305f\u3081\u306eRNA\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u6df7\u5165\u3057\u305f\u30b2\u30ce\u30e0DNA\u3092\u53d6\u308a\u9664\u304f\u3053\u3068\u304c\u3067\u304d\u308b\u306e\u3067\u3059\u304b\u3002"},"content":{"rendered":"\n<p>40 mM Tris-HCl (pH 7.9)\u300110 mM NaCl\u30016 mM MgCl2\u300110 mM CaCl2\u3092\u542b\u3080\u30d0\u30c3\u30d5\u30a1\u30fc\u4e2d\u3067\u6700\u3082\u9ad8\u3044\u6d3b\u6027\u304c\u5f97\u3089\u308c\u307e\u3059\u3002RQ1 RNase-Free DNase\u3092\u4f7f\u3046\u3068\u3001RT-PCR<sup>(a)<\/sup>\u3092\u5b9f\u65bd\u3059\u308b\u524d\u306b\u3001\u30c8\u30fc\u30bf\u30ebRNA\u3001poly(A)+ RNA\u307e\u305f\u306f<em>in vitro<\/em>\u8ee2\u5199\u7523\u7269\u304b\u3089\u6df7\u5165\u3057\u305f\u30b2\u30ce\u30e0(\u307e\u305f\u306f\u30d7\u30e9\u30b9\u30df\u30c9)DNA\u3092\u9664\u304f\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u9175\u7d20\u306f\u3001\u6700\u5927\u306e\u6d3b\u6027\u3092\u5f97\u308b\u305f\u3081\u306b\u30ab\u30eb\u30b7\u30a6\u30e0\u3084\u30de\u30b0\u30cd\u30b7\u30a6\u30e0\uff0f\u30de\u30f3\u30ac\u30f3\u3092\u5fc5\u8981\u3068\u3057(1)\u3001\u4e00\u822c\u7684\u306b1\u03bcg\u306eDNA(\u307e\u305f\u306fRNA)\u306b\u5bfe\u3057\u3066\u30011\u30e6\u30cb\u30c3\u30c8\u306eRQ1 RNase-Free DNase\u304c\u4f7f\u308f\u308c\u307e\u3059\u3002\u69d8\u3005\u306a\u30d0\u30c3\u30d5\u30a1\u30fc(\u5236\u9650\u9175\u7d20\u3001\u30ea\u30ac\u30fc\u30bc\u3001RT(reverse transcriptase)\u3001CIAP(calf intestinal alkaline phosphatase)\u4e2d\u3067\u5207\u65ad\u3092\u884c\u3048\u307e\u3059\u3002\u3057\u304b\u3057\u300140mM Tris-HCl(pH 7.9)\u300110mM NaCl\u30016mM MgCl<sub>2<\/sub>\u300110mM CaCl<sub>2<\/sub>\u3092\u542b\u3080\u30d0\u30c3\u30d5\u30a1\u30fc\u4e2d\u3067\u6700\u3082\u9ad8\u3044\u6d3b\u6027\u304c\u5f97\u3089\u308c\u307e\u3059\u3002\u4e07\u304c\u4e00\u306eRNA\u306e\u5206\u89e3\u3092\u9632\u3050\u305f\u3081\u306bRNasin<sup>\u00ae<\/sup>&nbsp;Ribonuclease Inhibitor\u3092\u5165\u308c\u308b\u3053\u3068\u3082\u3067\u304d\u307e\u3059\u300237\u2103\u300115~30\u5206\u9593\u306e\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u306e\u5f8c\u3001\u30d5\u30a7\u30ce\u30fc\u30eb\uff0f\u30af\u30ed\u30ed\u30d5\u30a9\u30eb\u30e0\uff0f\u30a4\u30bd\u30a2\u30df\u30eb\u30a2\u30eb\u30b3\u30fc\u30eb\u62bd\u51fa\u3068\u30a8\u30bf\u30ce\u30fc\u30eb\u6c88\u6bbf\u3084\u3001ReliaPrep\u2122 RNA Clean-Up and Concentration System (\u30ab\u30bf\u30ed\u30b0\u756a\u53f7 Z1071\u306a\u3069)\u3067RQ1 RNase-Free DNase\u3092\u9664\u53bb\u3057\u307e\u3059\u3002\u3055\u3089\u306b\u3001RT-PCR\u3067\u4f7f\u7528\u3059\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u306f\u3001\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8RNA\u3068\u30b2\u30ce\u30e0DNA\u304b\u3089\u306e\u751f\u7523\u7269\u3092\u533a\u5225\u3067\u304d\u308b\u3088\u3046\u306b\u30a4\u30f3\u30c8\u30ed\u30f3\u3092\u306f\u3055\u3080\u3088\u3046\u306b\u30c7\u30b6\u30a4\u30f3\u3057\u307e\u3059\u3002\uff08PN68-FAQ)<\/p>\n\n\n\n<p><strong>\u53c2\u8003\u6587\u732e\uff1a<\/strong><\/p>\n\n\n\n<p>1)Ausubel, F.M. et al. (1994) In:&nbsp;<em>Current Protocols in Molecular Biology<\/em>, John Wiley and Sons, New York, 3.12.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>40 mM Tris-HCl (pH 7.9)\u300110 mM NaCl\u30016 mM MgCl2\u300110 mM CaCl2\u3092\u542b\u3080\u30d0\u30c3\u30d5\u30a1\u30fc\u4e2d\u3067\u6700\u3082\u9ad8\u3044\u6d3b\u6027\u304c\u5f97\u3089\u308c\u307e\u3059\u3002RQ1 RNase-Free DNase\u3092\u4f7f\u3046\u3068\u3001RT-PC &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[127],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/532"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=532"}],"version-history":[{"count":1,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/532\/revisions"}],"predecessor-version":[{"id":533,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/532\/revisions\/533"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=532"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=532"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=532"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}