{"id":646,"date":"2020-12-02T16:53:34","date_gmt":"2020-12-02T07:53:34","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=646"},"modified":"2021-01-27T15:27:34","modified_gmt":"2021-01-27T06:27:34","slug":"q440","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/genetic-reporter\/firefly-luc\/dual-glo-and-dual-luc\/q440","title":{"rendered":"\u30a6\u30df\u30b7\u30a4\u30bf\u30b1\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u306e\u6d3b\u6027\u306f\u3001\u30db\u30bf\u30eb\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u306e\u6d3b\u6027\u3068\u3069\u306e\u3088\u3046\u306a\u9055\u3044\u304c\u3042\u308a\u307e\u3059\u304b? \u307e\u305f\u3001\uff12\u3064\u306e\u30ec\u30dd\u30fc\u30bf\u30fc\u30d9\u30af\u30bf\u30fc\u306e\u5171\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u306f\u3069\u306e\u3088\u3046\u306a\u6761\u4ef6\u304c\u63a8\u5968\u3055\u308c\u307e\u3059\u304b\u3002"},"content":{"rendered":"\n<p>\u30db\u30bf\u30eb\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u306fATP\u3001\u30de\u30b0\u30cd\u30b7\u30a6\u30e0\u3001\u304a\u3088\u3073\u9178\u7d20\u306e\u5b58\u5728\u4e0b\u3067\u30eb\u30b7\u30d5\u30a7\u30ea\u30f3\u306b\u4f5c\u7528\u3057\u307e\u3059\u3002\u30a6\u30df\u30b7\u30a4\u30bf\u30b1Renilla 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Assay System\u3092\u7528\u3044\u3066\u7d4c\u9a13\u7684\u306b\u308f\u304b\u3063\u3066\u3044\u307e\u3059\u3002\u8a73\u3057\u304f\u306f\u53c2\u8003\u8cc7\u6599\uff081\uff09\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<p>\u5171\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3055\u308c\u305f\u30d7\u30e9\u30b9\u30df\u30c9\u4e0a\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u9593\u306b\u8d77\u3053\u308b\u30c8\u30e9\u30f3\u30b9\u52b9\u679c\u306f\u30ec\u30dd\u30fc\u30bf\u30fc\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u306b\u5f37\u304f\u5f71\u97ff\u3057\u307e\u3059\uff082\uff09\u3002\u3053\u308c\u306f\u3001\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u307e\u305f\u306f\u5b9f\u9a13\u7528\u30ec\u30dd\u30fc\u30bf\u30fc\u30d9\u30af\u30bf\u30fc\u304c\u975e\u5e38\u306b\u5f37\u3044\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\uff0f\u30a8\u30f3\u30cf\u30f3\u30b5\u30fc\u30a8\u30ec\u30e1\u30f3\u30c8\u3092\u542b\u3080\u3068\u304d\u306b\u3001\u7279\u306b\u6ce8\u610f\u3057\u306a\u3051\u308c\u3070\u306a\u308a\u307e\u305b\u3093\u3002phRL 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early\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\uff0f\u30a8\u30f3\u30cf\u30f3\u30b5\u30fc\u9818\u57df\u306f\u3001\u9ad8\u30ec\u30d9\u30eb\u306e\u8ee2\u5199\u6d3b\u6027\u304c\u3042\u308a\u3001\u5f37\u3044\u8abf\u7bc0\u56e0\u5b50\u3092\u6301\u3064\u5b9f\u9a13\u30d9\u30af\u30bf\u30fc\u3092\u542b\u3080\u91cd\u30ec\u30dd\u30fc\u30bf\u30fc\u306e\u5b9f\u9a13\u306b\u306f\u5411\u304b\u306a\u3044\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u3055\u3089\u306b\u3001\uff33\uff3640\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3068\uff23\uff2d\uff36\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306b\u306f\u3001\uff33\uff301\u3001\uff21\uff301\u304a\u3088\u3073\uff21\uff302\u306e\u8ee2\u5199\u56e0\u5b50\u8a8d\u8b58\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u304c\u542b\u307e\u308c\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u8ee2\u5199\u56e0\u5b50\u306e\u5b9f\u9a13\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306b\u5bfe\u3059\u308b\u5f71\u97ff\u3092\u8abf\u3079\u3066\u3044\u308b\u306e\u3067\u3042\u308c\u3070\u3001\uff33\uff3640\u304a\u3088\u3073\uff23\uff2d\uff36\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30ec\u30dd\u30fc\u30bf\u30fc\u306e\u4f7f\u7528\u306f\u63a8\u5968\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n\n\n\n<p>\u5b9f\u9a13\u7528\u3068\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306e\u30ec\u30dd\u30fc\u30bf\u30fc\u907a\u4f1d\u5b50\u9593\u3067\u72ec\u7acb\u3057\u305f\u907a\u4f1d\u5b50\u767a\u73fe\u3092\u78ba\u5b9f\u306b\u3059\u308b\u305f\u3081\u3001\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u30a8\u30ec\u30e1\u30f3\u30c8\u9593\u306e\u30c8\u30e9\u30f3\u30b9\u52b9\u679c\u3092\u6e1b\u5c11\u3055\u305b\u308b\uff08\u307e\u305f\u306f\u6291\u5236\u3059\u308b\uff09\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u307e\u305f\u5171\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3055\u308c\u305f\u30d9\u30af\u30bf\u30fc\u306e\u767a\u73fe\u30ec\u30d9\u30eb\u3092\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3059\u308b\u305f\u3081\u306b\u3001\u5b9f\u9a13\u7528\u306e\u30d9\u30af\u30bf\u30fc\u306e\u30a6\u30df\u30b7\u30a4\u30bf\u30b1\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u30d9\u30af\u30bf\u30fc\u306b\u5bfe\u3059\u308b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6bd4\u7387\u306f10\uff1a1\u300150\uff1a1\u3001200\uff1a1\u307e\u305f\u306f\u305d\u308c\u4ee5\u4e0a\u304c\u63a8\u5968\u3055\u308c\u307e\u3059\u3002\u8a73\u3057\u304f\u306fDual-Luciferase<sup>\u00ae<\/sup>\u00a0Reporter Assay System\u00a0<a href=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/dual-luciferase-reporter-assay-system-protocol.pdf\" data-type=\"URL\" target=\"_blank\" rel=\"noreferrer noopener\">Technical Manual #TM040<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<p>\u53c2\u8003\u6587\u732e\uff1a<\/p>\n\n\n\n<p>1.Sherf,B.A. et al. (1996) Promega Notes 57,2<\/p>\n\n\n\n<p>2.Farr,A. and Roman,A. (1991) Nucl. Acids Res. 20,920<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30db\u30bf\u30eb\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u306fATP\u3001\u30de\u30b0\u30cd\u30b7\u30a6\u30e0\u3001\u304a\u3088\u3073\u9178\u7d20\u306e\u5b58\u5728\u4e0b\u3067\u30eb\u30b7\u30d5\u30a7\u30ea\u30f3\u306b\u4f5c\u7528\u3057\u307e\u3059\u3002\u30a6\u30df\u30b7\u30a4\u30bf\u30b1Renilla reniformis\u304b\u3089\u5f97\u3089\u308c\u305f\u30a6\u30df\u30b7\u30a4\u30bf\u30b1\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u306f\u3001\u9178\u7d20\u306e\u5b58\u5728\u4e0b\u3067\u30bb\u30ec\u30f3\u30c6\u30e9\u30b8\u30f3\u306b\u4f5c\u7528\u3057\u307e\u3059 &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[183],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/646"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=646"}],"version-history":[{"count":3,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/646\/revisions"}],"predecessor-version":[{"id":2442,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/646\/revisions\/2442"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=646"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=646"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=646"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}