{"id":649,"date":"2020-12-02T16:55:27","date_gmt":"2020-12-02T07:55:27","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=649"},"modified":"2021-01-27T15:30:12","modified_gmt":"2021-01-27T06:30:12","slug":"q441","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/genetic-reporter\/firefly-luc\/dual-glo-and-dual-luc\/q441","title":{"rendered":"\u30ec\u30dd\u30fc\u30bf\u30fc\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u7d50\u679c\u3092\u6a19\u6e96\u5316\u3059\u308b\u3053\u3068\u306f\u3001\u306a\u305c\u5fc5\u8981\u306a\u306e\u3067\u3059\u304b\uff1f\u307e\u305f\u3001\u3069\u306e\u3088\u3046\u306a\u5185\u90e8\u6a19\u6e96\u30d9\u30af\u30bf\u30fc\u3092\u4f7f\u3046\u306e\u304c\u6700\u3082\u826f\u3044\u3067\u3059\u304b\uff1f"},"content":{"rendered":"\n

\u771f\u6838\u7d30\u80de\u306b\u304a\u3051\u308b\u4e00\u904e\u7684\u306a\u30ec\u30dd\u30fc\u30bf\u30fc\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u306f\u3001\u907a\u4f1d\u5b50\u767a\u73fe\u306e\u69d8\u3005\u306a\u5074\u9762\u3092\u5206\u6790\u3059\u308b\u5f37\u529b\u306a\u30c4\u30fc\u30eb\u3067\u3059\u3002\u591a\u304f\u306e\u5834\u5408\u3001\u907a\u4f1d\u5b50\u767a\u73fe\u306e\u5236\u5fa1\u306b\u95a2\u308f\u308b\u8abf\u7bc0\u56e0\u5b50\u306f\u3001\u30ec\u30dd\u30fc\u30bf\u30fc\u30d9\u30af\u30bf\u30fc\u306b\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3055\u308c\u3001\u8abf\u7bc0\u56e0\u5b50\u304c\u907a\u4f1d\u5b50\u767a\u73fe\u306b\u53ca\u307c\u3059\u5f71\u97ff\u3092\u8abf\u3079\u308b\u305f\u3081\u306b\u3053\u308c\u3089\u306e\u30ad\u30e1\u30e9\u30d9\u30af\u30bf\u30fc\u304c\u7d30\u80de\u306b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30c8\u3055\u308c\u307e\u3059\u3002\u4e00\u822c\u7684\u306a\u30ec\u30dd\u30fc\u30bf\u30fc\u907a\u4f1d\u5b50\u306b\u306f\u3001\u30db\u30bf\u30eb\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u3084\u3001\u30af\u30ed\u30e9\u30e0\u30d5\u30a7\u30cb\u30b3\u30fc\u30eb\u30fb\u30a2\u30bb\u30c1\u30eb\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30e9\u30fc\u30bc\uff08CAT\uff09\u3001\u30b0\u30ea\u30fc\u30f3\u30fb\u30d5\u30eb\u30aa\u30ec\u30bb\u30f3\u30c8\u30fb\u30d7\u30ed\u30c6\u30a4\u30f3\uff08GFP\uff09\u3001\u03b2\u30fc\u30ac\u30e9\u30af\u30c8\u30b7\u30c0\u30fc\u30bc\u304c\u542b\u307e\u308c\u307e\u3059\u3002\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30c8\u3055\u308c\u305f\u7d30\u80de\u306b\u3055\u307e\u3056\u307e\u306a\u51e6\u7406\u3092\u4e0e\u3048\u308b\u3053\u3068\u3067\u3001\u3053\u308c\u3089\u306e\u51e6\u7406\u304c\u30ec\u30dd\u30fc\u30bf\u30fc\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u306b\u53ca\u307c\u3059\u5f71\u97ff\u3092\u6e2c\u5b9a\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n

\u307b\u304b\u306e\u8981\u56e0\u3082\u4e00\u6642\u7684\u306a\u767a\u73fe\u306e\u7814\u7a76\u304b\u3089\u5f97\u3089\u308c\u308b\u7d50\u679c\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u8981\u56e0\u306b\u306f\u3001\u30ec\u30dd\u30fc\u30bf\u30fc\u30fb\u30a2\u30c3\u30bb\u30a4\u8a66\u85ac\u306e\u591a\u69d8\u6027\u3068\u5171\u306b\u3001\uff081\uff09\u57f9\u990a\u7d30\u80de\u306e\u6570\u3084\u5065\u5eb7\u5ea6\u306e\u9055\u3044\u3001\uff082\uff09\u7d30\u80de\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u3084\uff083\uff09\u7d30\u80de\u6eb6\u89e3\u306e\u52b9\u7387\u306e\u9055\u3044\u304c\u542b\u307e\u308c\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u8981\u56e0\u3092\u88dc\u3046\u305f\u3081\u306b\u30012\u756a\u76ee\u306e\u30ec\u30dd\u30fc\u30bf\u30fc\u30d9\u30af\u30bf\u30fc\u304c\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u5b9f\u9a13\u306b\u53d6\u308a\u5165\u308c\u3089\u308c\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u5b9f\u9a13\u306e\u305f\u3081\u306e\u30ec\u30dd\u30fc\u30bf\u30fc\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u304c\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30fb\u30ec\u30dd\u30fc\u30bf\u30fc\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u3067\u6a19\u6e96\u5316\u3055\u308c\u307e\u3059\u3002\u6a19\u6e96\u5316\u306e\u305f\u3081\u306e\u30d9\u30af\u30bf\u30fc\u306f\u3001\u5b9f\u9a13\u306e\u305f\u3081\u306e\u30ec\u30dd\u30fc\u30bf\u30fc\u30d9\u30af\u30bf\u30fc\u306b\u5bfe\u3057\u3066\u884c\u308f\u308c\u308b\u51e6\u7406\u306b\u53cd\u5fdc\u3057\u306a\u3044\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306e\u8ee2\u5199\u8abf\u7bc0\u4e0b\u306b\u3001\u30ec\u30dd\u30fc\u30bf\u30fc\u907a\u4f1d\u5b50\u3092\u542b\u307f\u307e\u3059\u3002\u5b9f\u9a13\u306e\u305f\u3081\u306e\u30ec\u30dd\u30fc\u30bf\u30fc\u306e\u6d3b\u6027\u3092\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30fb\u30ec\u30dd\u30fc\u30bf\u30fc\u306e\u6d3b\u6027\u3067\u6a19\u6e96\u5316\u3059\u308b\u3053\u3068\u3067\u3001\u30ec\u30dd\u30fc\u30bf\u30fc\u30a2\u30c3\u30bb\u30a4\u3067\u554f\u984c\u3068\u3055\u308c\u308b\u30a2\u30c3\u30bb\u30a4\u6bce\u306e\u3070\u3089\u3064\u304d\u3092\u53d6\u308a\u9664\u304d\u307e\u3059\u3002
\u4ee5\u4e0b\u306e\u8868\u306f\u3001Dual-Luciferase\u00ae<\/sup> Reporter Assay System\u3092\u4f7f\u3063\u3066\u3044\u308b\u5178\u578b\u7684\u306a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u5b9f\u9a13\u3067\u5f97\u3089\u308c\u308b\u30c7\u30fc\u30bf\u306e\u62bd\u51fa\u6a19\u672c\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002
<\/p>\n\n\n\n

\u8868\u3000Dual-LuciferaseR Assay System\u3092\u7528\u3044\u305f\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u5b9f\u9a13\u306b\u304a\u3051\u308b\u6d3b\u6027\u5b9a\u91cf<\/strong><\/p>\n\n\n\n

\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n
Day1<\/strong><\/td>\n <\/tr>\n
Treatment
Replicate<\/th>\n 		Luciferase Activity (RLU)<\/td>\n Ratio
(F:R)<\/td>\n 		Normalized Fold
Change in Activity<\/td>\n <\/tr>\n
Firefly (F)<\/td>\n Renilla (R)<\/td>\n <\/tr>\n
Control<\/th>\n \u3000<\/td>\n \u3000<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
1<\/th>\n 5,128<\/td>\n 2,511<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
2<\/th>\n 7,553<\/td>\n 3,815<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
3<\/th>\n 10,555<\/td>\n 5,413<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
\u3000<\/th>\n 7,745<\/strong><\/td>\n 3,913<\/strong><\/td>\n 1.98<\/strong><\/td>\n 1.00<\/strong><\/td>\n <\/tr>\n
Treatment A<\/th>\n \u3000<\/td>\n \u3000<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
1<\/th>\n 51,376<\/td>\n 4,467<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
2<\/th>\n 40,712<\/td>\n 3,574<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
3<\/th>\n 88,787<\/td>\n 7,654<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
\u3000<\/th>\n 60,292<\/strong><\/td>\n 5,232<\/strong><\/td>\n 11.52<\/strong><\/td>\n 5.82<\/strong><\/td>\n <\/tr>\n
Treatment B<\/th>\n \u3000<\/td>\n \u3000<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
1<\/th>\n 587,635<\/td>\n 5,144<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
2<\/th>\n 988,347<\/td>\n 8,832<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
3<\/th>\n 409,881<\/td>\n 3,564<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
\u3000<\/th>\n 661,954<\/strong><\/td>\n 5,847<\/strong><\/td>\n 113.21<\/strong><\/td>\n 57.18<\/strong><\/td>\n <\/tr>\n
Control treatment = no treatment; RLU = relative light units, Except for the final column, values in boldface type are averages.<\/td>\n <\/tr>\n <\/tbody>\n <\/table>\n<\/figure>\n\n\n\n
\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n
Day2<\/strong><\/td>\n <\/tr>\n
Treatment
Replicate<\/th>\n 		Luciferase Activity (RLU)<\/td>\n Ratio
(F:R)<\/td>\n 		Normalized Fold
Change in Activity<\/td>\n <\/tr>\n
Firefly (F)<\/td>\n Renilla (R)<\/td>\n <\/tr>\n
Control<\/th>\n \u3000<\/td>\n \u3000<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
1<\/th>\n 15,529<\/td>\n 20,433<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
2<\/th>\n 21,897<\/td>\n 30,841<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
3<\/th>\n 10,760<\/td>\n 13,620<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
\u3000<\/th>\n 16,062<\/strong><\/td>\n 21,631<\/strong><\/td>\n 0.74<\/strong><\/td>\n 1.00<\/strong><\/td>\n <\/tr>\n
Treatment C<\/th>\n \u3000<\/td>\n \u3000<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
1<\/th>\n 850,239<\/td>\n 20,843<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
2<\/th>\n 578,951<\/td>\n 14,830<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
3<\/th>\n 943,873<\/td>\n 21,788<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
\u3000<\/th>\n 791,021<\/strong><\/td>\n 19,154<\/strong><\/td>\n 41.30<\/strong><\/td>\n 55.81<\/strong><\/td>\n <\/tr>\n
Treatment D<\/th>\n \u3000<\/td>\n \u3000<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
1<\/th>\n 14,865<\/td>\n 19,305<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
2<\/th>\n 8,967<\/td>\n 12,284<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
3<\/th>\n 13,767<\/td>\n 20,246<\/td>\n \u3000<\/td>\n \u3000<\/td>\n <\/tr>\n
\u3000<\/th>\n 12,533<\/strong><\/td>\n 17,278<\/strong><\/td>\n 0.73<\/strong><\/td>\n 0.99<\/strong><\/td>\n <\/tr>\n
Control treatment = no treatment; RLU = relative light units, Except for the final column, values in boldface type are averages.<\/td>\n <\/tr><\/tbody><\/table><\/figure>\n\n\n\n


\u30c8\u30ea\u30d7\u30ea\u30b1\u30fc\u30c8\u3067\u540c\u3058\u7a2e\u985e\u306e\u7d30\u80de\u306b\u3001\u540c\u3058\u30db\u30bf\u30eb\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u907a\u4f1d\u5b50\u3092\u542b\u3080\u3001\u5b9f\u9a13\u306e\u305f\u3081\u306e\u30ec\u30dd\u30fc\u30bf\u30fc\u30d9\u30af\u30bf\u30fc\u3068\u3001\u5185\u90e8\u6a19\u6e96\u3068\u3057\u3066TK(tymidine kinase)\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306e\u652f\u914d\u4e0b\u306b\u3042\u308b\u30a6\u30df\u30b7\u30a4\u30bf\u30b1\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u907a\u4f1d\u5b50\u3092\u542b\u3080\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30fb\u30ec\u30dd\u30fc\u30bf\u30fc\u30fb\u30d9\u30af\u30bf\u30fc\u3092\u5171\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3057\u307e\u3057\u305f\u3002\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u306e\u30ec\u30dd\u30fc\u30bf\u30fc\u6d3b\u6027\u306f\u3001\u540c\u3058\u7d44\u306e\u4e2d\u3067\u304b\u306a\u308a\u5927\u304d\u304f\u5909\u308f\u308a\u307e\u3059\uff08\u4f8b\u3048\u3070Day2\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306e\u30db\u30bf\u30eb\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u306e\u6d3b\u6027\u306f10,000\uff5e21,000RLU\u306e\u7bc4\u56f2\u306b\u3042\u308a\u307e\u3059\uff09\u3002\u3057\u304b\u3057\u3001\u30db\u30bf\u30eb\u3068\u30a6\u30df\u30b7\u30a4\u30bf\u30b1\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u306e\u6d3b\u6027\u306e\u6bd4\u7387\u3092\u6c42\u3081\u308b\u3068\u3001\u69d8\u3005\u306a\u30ec\u30d7\u30ea\u30b1\u30fc\u30c8\u306e\u5024\u304c\u6a19\u6e96\u5316\u3055\u308c\u3001\u304a\u4e92\u3044\u306b\u8fd1\u4f3c\u306e\u6bd4\u7387\u3068\u306a\u308a\u307e\u3059\uff08\u4f8b\u3048\u3070\u30010.76\u30010.71\u30010.79\uff09\u3002<\/p>\n\n\n\n

\u4e0b\u8a18\u306e\u7b49\u5f0f\u306f\u3001\u6d3b\u6027\u306e\u6a19\u6e96\u5316\u3057\u305f\u500d\u7387\u306e\u5909\u5316\u3092\u6c7a\u3081\u308b\u305f\u3081\u306b\u4f7f\u308f\u308c\u307e\u3059\u3002<\/strong><\/p>\n\n\n\n

\n \n \n \n \n \n \n \n
<\/td>\n (F\/R)sample<\/td>\n <\/tr>\n
\u0394Fold Activity =<\/td>\n \u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015<\/td>\n <\/tr>\n
<\/td>\n (F\/R)control<\/td>\n <\/tr>\n
<\/td>\n \u3000<\/td>\n <\/tr>\n
F\u306f\u30db\u30bf\u30eb\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u306e\u6d3b\u6027\u3067\u3001R\u306f\u30a6\u30df\u30b7\u30a4\u30bf\u30b1\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u306e\u6d3b\u6027<\/td>\n <\/tr><\/tbody><\/table><\/figure>\n\n\n\n

\u4f8b\u3048\u3070\u3001Day2\u306e\u51e6\u7406C\u306f\u3001\u6b21\u306e\u3088\u3046\u306b\u306a\u308a\u307e\u3059\u3002<\/strong><\/p>\n\n\n\n

\n \n \n \n \n \n \n \n \n \n \n \n
<\/td>\n (791,021\/19,154)<\/td>\n <\/tr>\n
\u0394Fold Activity =<\/td>\n \u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015<\/td>\n <\/tr>\n
<\/td>\n (16,062\/21,631)<\/td>\n <\/tr>\n
<\/td>\n <\/td>\n <\/tr>\n
<\/td>\n (41.30)<\/td>\n <\/tr>\n
=<\/td>\n \u2015\u2015\u2015\u2015\u2015<\/td>\n <\/tr>\n
<\/td>\n (0.74)<\/td>\n <\/tr>\n
<\/td>\n <\/td>\n <\/tr>\n
=<\/td>\n 55.81<\/td>\n <\/tr><\/tbody><\/table><\/figure>\n\n\n\n

\u30db\u30bf\u30eb\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u3068\u30a6\u30df\u30b7\u30a4\u30bf\u30b1\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u306e\u6d3b\u6027\u306e\u6bd4\u7387\u304c\u5b9f\u9a13\u9593\u3067\u7570\u306a\u308b\u305f\u3081\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u5b9f\u9a13\u9593\u306e\u30c7\u30fc\u30bf\u3092\u6bd4\u8f03\u3059\u308b\u306b\u306f\u3001\u5171\u901a\u306e\u30b5\u30f3\u30d7\u30eb\uff08\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u7e70\u308a\u8fd4\u3057\uff09\u304c\u542b\u307e\u308c\u308b\u3088\u3046\u306b\u3057\u307e\u3059\u3002<\/p>\n\n\n\n

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<\/p>\n","protected":false},"excerpt":{"rendered":"

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