{"id":682,"date":"2020-12-02T17:04:17","date_gmt":"2020-12-02T08:04:17","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=682"},"modified":"2021-01-14T14:45:37","modified_gmt":"2021-01-14T05:45:37","slug":"q447","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/genetic-reporter\/firefly-luc\/dual-glo-and-dual-luc\/q447","title":{"rendered":"\u30db\u30bf\u30eb\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u3068\u30a6\u30df\u30b7\u30a4\u30bf\u30b1\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u306e\u767a\u5149\u5024\u306b\u3070\u3089\u3064\u304d\u304c\u898b\u3089\u308c\u307e\u3059\u3002\u539f\u56e0\u3068\u3057\u3066\u4f55\u304c\u8003\u3048\u3089\u308c\u307e\u3059\u304b\uff1f"},"content":{"rendered":"\n

\u6b21\u306e\u9805\u76ee\u306b\u3064\u3044\u3066\u518d\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\u3008\u7d30\u80de\u3001\u8a66\u85ac\u3009<\/strong><\/p>\n\n\n\n

  1. \u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u4f7f\u7528\u3059\u308b\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u7d14\u5ea6\u306f\uff1f(A260\/A280 1.8-1.9\u7a0b\u5ea6\u3067\u3059\u304b\uff1f)<\/li>
  2. \u7d30\u80de\u306ePBS(-)\u6d17\u6d44\u64cd\u4f5c\u3092\u5341\u5206\u306b\u884c\u3063\u3066\u304f\u3060\u3055\u3044\u3002(\u57f9\u5730\u6210\u5206\u306eMg2+\u3084Ca2+\u306f\u3001\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u53cd\u5fdc\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u307e\u3059\u3002)<\/li>
  3. \u7d30\u80de\u306e\u6eb6\u89e3\u306f\u5341\u5206\u3067\u3059\u304b\uff1f\uff08\u6eb6\u89e3\u3057\u96e3\u3044\u5834\u5408\u306f\u3001\u51cd\u7d50\u878d\u89e3\u3092\u884c\u3063\u3066\u304f\u3060\u3055\u3044\u3002\uff09<\/li>
  4. \u8a66\u85ac\u306e\u8abf\u88fd\u306f\u6b63\u3057\u304f\u884c\u3063\u3066\u3044\u307e\u3059\u304b\uff1f\uff08Stop & Glo\u00ae<\/sup> Reagent\u306e\u8abf\u88fd\u306f\u3001\u57fa\u8cea\u304c\u30c1\u30e5\u30fc\u30d6\u5185\u58c1\u306b\u5438\u7740\u3059\u308b\u6050\u308c\u304c\u3042\u308a\u307e\u3059\u306e\u3067\u3001\u30ac\u30e9\u30b9\u3042\u308b\u3044\u306f\u30b7\u30ea\u30b3\u30f3\u30b3\u30fc\u30c8\u3057\u305f\u30c1\u30e5\u30fc\u30d6\u3067\u884c\u3063\u3066\u304f\u3060\u3055\u3044\uff09<\/li>
  5. \u4f7f\u7528\u524d\u306b\u8a66\u85ac\u306f\u5747\u4e00\u306b\u306a\u3063\u3066\u3044\u307e\u3059\u304b\uff1f\uff08\u51cd\u7d50\u4fdd\u5b58\u3057\u305f\u8a66\u85ac\u306f\u3001\u6fc3\u5ea6\u52fe\u914d\u304c\u751f\u3058\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u5341\u5206\u306a\u6df7\u5408\u304c\u5fc5\u8981\u3067\u3059\u3002\uff09<\/li>
  6. \u30a2\u30c3\u30bb\u30a4\u306b\u4f7f\u7528\u3059\u308b\u8a66\u85ac\u3001\u7d30\u80de\u30e9\u30a4\u30bb\u30fc\u30c8\u306f\u3001\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u53cd\u5fdc\u306b\u9069\u3057\u305f\u5ba4\u6e29\u306b\u306a\u3063\u3066\u3044\u307e\u3059\u304b\uff1f<\/li>
  7. pGL3 Control Vector(\u30ab\u30bf\u30ed\u30b0\u756a\u53f7\uff1aE1741)\u3092\u7528\u3044\u3066\u3001\u304a\u4f7f\u3044\u306b\u306a\u308b\u7d30\u80de\u3084\u6e2c\u5b9a\u6a5f\u5668\u3067\u3001\u6e2c\u5b9a\u53ef\u80fd\u7bc4\u56f2\u5185\u306e\u767a\u5149\u91cf\u304c\u5f97\u3089\u308c\u308b\u6761\u4ef6\u3092\u3054\u691c\u8a0e\u304f\u3060\u3055\u3044\u3002<\/li><\/ol>\n\n\n\n

    \u3008\u6a5f\u5668\u3009<\/strong><\/p>\n\n\n\n

    1. QuantiLum\u00ae<\/sup> Recombinant Luciferase(\u30ab\u30bf\u30ed\u30b0\u756a\u53f7\uff1aE1701)\u3092\u7528\u3044\u3066\u3001\u304a\u4f7f\u3044\u306e\u30eb\u30df\u30ce\u30e1\u30fc\u30bf\u30fc\u306e\u6e2c\u5b9a\u53ef\u80fd\u7bc4\u56f2\uff08\u30ea\u30cb\u30a2\u30ec\u30f3\u30b8\uff09\u3092\u6c7a\u5b9a\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u30b5\u30f3\u30d7\u30eb\u306e\u767a\u5149\u5024\u306f\u3001\u30ea\u30cb\u30a2\u30ec\u30f3\u30b8\u5185\u3067\u3042\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>
    2. \u86cd\u5149\/\u767a\u5149\u30de\u30eb\u30c1\u30bf\u30a4\u30d7\u306e\u6e2c\u5b9a\u6a5f\u5668\u306e\u5834\u5408\u306f\u3001\u6b63\u3057\u3044\u8a2d\u5b9a\u3067\u3042\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li><\/ol>\n","protected":false},"excerpt":{"rendered":"

      \u6b21\u306e\u9805\u76ee\u306b\u3064\u3044\u3066\u518d\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002 \u3008\u7d30\u80de\u3001\u8a66\u85ac\u3009 \u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u4f7f\u7528\u3059\u308b\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u7d14\u5ea6\u306f\uff1f(A260\/A280 1.8-1.9\u7a0b\u5ea6\u3067\u3059\u304b\uff1f) \u7d30\u80de\u306ePBS(-)\u6d17\u6d44\u64cd\u4f5c\u3092\u5341\u5206\u306b\u884c\u3063\u3066\u304f\u3060\u3055\u3044\u3002(\u57f9\u5730\u6210\u5206\u306eM … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[183],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/682"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=682"}],"version-history":[{"count":1,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/682\/revisions"}],"predecessor-version":[{"id":685,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/682\/revisions\/685"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=682"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=682"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=682"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}